PLANT PHYSIOLOGY,
Год журнала:
2022,
Номер
189(1), С. 178 - 187
Опубликована: Фев. 17, 2022
Abstract
Efficient
plastid
transformation
in
Arabidopsis
(Arabidopsis
thaliana)
requires
genetic
lines
that
are
hypersensitive
to
spectinomycin
due
the
absence
of
a
chloroplast
acetyl-coenzyme
A
carboxylase
(ACCase)
encoded
2
(ACC2)
nuclear
gene.
To
obtain
transformation-competent
oilseed
rape
(Brassica
napus),
we
inactivated
all
encoded,
targeted
ACCase
copies
using
CRISPR–Cas9.
Brassica
napus
(2n
=
38,
AACC)
is
recent
interspecific
hybrid
rapa
20,
AA)
and
B.
oleracea
18,
CC)
expected
have
at
least
two
ACC2
copies,
one
from
each
parent.
The
sequenced
genome
has
rapa-like
oleracea-like.
We
designed
single-guide
RNAs
(sgRNAs)
could
simultaneously
inactivate
both
copies.
expressed
Cas9
chimeric
egg
cell
promoter
1.2
(EC1.2p)
known
yield
homozygous
or
biallelic
mutants
T1
generation.
maximize
probability
functionally
inactivating
orthologs
single
step,
vectors
carried
four
sgRNAs.
Four
T0
transgenic
were
obtained
by
Agrobacterium
tumefaciens-mediated
hypocotyl
transformation.
Amplicon
sequencing
confirmed
mutations
genes
10
progeny,
seven
which
no
wild-type
(WT)
copy
remained.
T2
seedlings
lacking
WT
gene
exhibited
phenotype,
suggesting
they
will
be
useful
resource
for
Annual Review of Entomology,
Год журнала:
2021,
Номер
67(1), С. 239 - 259
Опубликована: Окт. 4, 2021
Bottom-up
effects
are
major
ecological
forces
in
crop-arthropod
pest-natural
enemy
multitrophic
interactions.
Over
the
past
two
decades,
bottom-up
have
been
considered
key
levers
for
optimizing
integrated
pest
management
(IPM).
Irrigation,
fertilization,
crop
resistance,
habitat
manipulation,
organic
practices,
and
landscape
characteristics
all
shown
to
trigger
marked
thus
impact
management.
In
this
review,
we
summarize
current
knowledge
on
role
of
associated
mechanisms,
discuss
several
study
cases
showing
how
practically
promote
natural
control.
IPM
also
contribute
sustainable
intensification
agriculture
context
agricultural
transition
climate
change.
Finally,
highlight
new
research
priorities
important
area.
Together
with
top-down
(biological
control),
future
advances
understanding
mechanisms
underlying
could
pave
way
developing
novel
strategies
optimized
programs.
Frontiers in Plant Science,
Год журнала:
2022,
Номер
13
Опубликована: Май 18, 2022
The
development
of
plant
varieties
with
desired
traits
is
imperative
to
ensure
future
food
security.
revolution
genome
editing
technologies
based
on
the
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/CRISPR-associated
nuclease
9
(Cas9)
system
has
ushered
in
a
new
era
breeding.
Cas9
and
single-guide
RNA
(sgRNA)
form
an
effective
targeting
complex
locus
or
loci
interest,
enabling
all
plants
high
accuracy
efficiency.
Therefore,
CRISPR/Cas9
can
save
both
time
labor
relative
what
typically
associated
traditional
breeding
methods.
However,
despite
improvements
gene
editing,
several
challenges
remain
that
limit
application
CRISPR/Cas9-based
plants.
Here,
we
focus
four
issues
relevant
editing:
(1)
organelle
editing;
(2)
transgene-free
(3)
virus-induced
(4)
recalcitrant
elite
crop
inbred
lines.
This
review
provides
up-to-date
summary
state
CRISPR/Cas9-mediated
will
push
this
technique
forward.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(3), С. 1326 - 1326
Опубликована: Янв. 25, 2022
Plants
offer
several
unique
advantages
in
the
production
of
recombinant
pharmaceuticals
for
humans
and
animals.
Although
numerous
proteins
have
been
expressed
plants,
only
a
small
fraction
successfully
put
into
use.
The
hugely
distinct
expression
systems
between
plant
animal
cells
frequently
cause
insufficient
yield
with
poor
or
undesired
activity.
To
overcome
issues
that
greatly
constrain
development
plant-produced
pharmaceuticals,
great
efforts
made
to
improve
develop
alternative
strategies
increase
both
quantity
quality
proteins.
Recent
technological
revolutions,
such
as
targeted
genome
editing,
deconstructed
vectors,
virus-like
particles,
humanized
glycosylation,
led
advances
molecular
farming
meet
industrial
manufacturing
clinical
application
standards.
In
this
review,
we
discuss
various
platforms,
special
focus
on
upstream
designs
milestone
achievements
improving
glycosylation
pharmaceutical
Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Фев. 7, 2023
The
acceptance
of
new
crop
varieties
by
consumers
is
contingent
on
the
presence
consumer-preferred
traits,
which
include
sensory
attributes,
nutritional
value,
industrial
products
and
bioactive
compounds
production.
Recent
developments
in
genome
editing
technologies
provide
novel
insight
to
identify
gene
functions
improve
various
qualitative
quantitative
traits
commercial
importance
plants.
Various
conventional
as
well
advanced
gene-mutagenesis
techniques
such
physical
chemical
mutagenesis,
CRISPR-Cas9,
Cas12
base
editors
are
used
for
trait
improvement
crops.
To
meet
consumer
demand,
breakthrough
biotechnologies,
especially
CRISPR-Cas
have
received
a
fair
share
scientific
interest,
particularly
plant
editing.
versatile
tool
that
can
be
knock
out,
replace
knock-in
desired
fragments
at
targeted
locations
genome,
resulting
heritable
mutations
interest.
This
review
highlights
existing
literature
recent
(base
editing,
prime
multiplex
epigenome
delivery
methods)
reliable
precise
also
discusses
potential
exhibited
crops
consumer-demanded
higher
colour,
texture,
aroma/flavour,
production
biofuel,
fibre,
rubber
pharmaceuticals.
In
addition,
bottlenecks
challenges
associated
with
system,
off
targeting,
ploidy
level
ability
edit
organelle
been
discussed.
Annual Review of Plant Biology,
Год журнала:
2022,
Номер
73(1), С. 173 - 200
Опубликована: Фев. 7, 2022
The
advent
of
high-throughput
sequencing-based
methods
for
chromatin
conformation,
accessibility,
and
immunoprecipitation
assays
has
been
a
turning
point
in
3D
genomics.
Altogether,
these
new
tools
have
pushing
upward
the
interpretation
pioneer
cytogenetic
evidence
higher
order
packing.
Here,
we
review
latest
development
our
understanding
plant
spatial
genome
structures
different
levels
organization
discuss
their
functional
implications.
Then,
spotlight
complexity
organellar
(i.e.,
mitochondria
plastids)
genomes
packing
into
nucleoids.
Finally,
propose
unaddressed
research
axes
to
investigate
links
between
chromatin-like
dynamics
transcriptional
regulation
within
GM crops & food,
Год журнала:
2023,
Номер
14(1), С. 1 - 27
Опубликована: Июнь 8, 2023
The
daunting
task
of
feeding
an
ever-growing
population
is
immense
challenge
for
the
contemporary
scientific
community,
especially
in
view
rapidly
changing
climate
throughout
world.
Amidst
these
threatening
crises,
we
witness
rapid
development
genome
editing
(GE)
technologies,
revolutionizing
field
applied
genomics
and
molecular
breeding.
Various
GE
tools
have
been
developed
during
last
two
decades,
but
CRISPR/Cas
system
has
most
recently
made
a
significant
impact
on
crop
improvement.
major
breakthroughs
this
versatile
toolbox
are
genomic
modifications
like
single
base-substitutions,
multiplex
GE,
gene
regulation,
screening
mutagenesis,
enhancing
breeding
wild
plants.
Previously,
was
used
to
modify
genes
related
traits
such
as
biotic/abiotic
resistance/tolerance,
post-harvest
traits,
nutritional
address
self-incompatibility
analysis-related
challenges.
In
present
review,
demonstrated
functional
dynamics
CRISPR-based
its
applicability
targeting
accomplish
novel
crops.
compiled
knowledge
will
provide
solid
foundation
highlighting
primary
source
applying
crops,
achieve
food
security.
