International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(12), С. 6603 - 6603
Опубликована: Июнь 15, 2024
Wheat
powdery
mildew
is
an
important
fungal
disease
that
seriously
jeopardizes
wheat
production,
which
poses
a
serious
threat
to
food
safety.
SJ106
high-quality,
disease-resistant
spring
variety;
this
resistance
derived
from
Wheat-wheatgrass
33.
In
study,
the
genes
in
were
located
at
end
of
chromosome
6DS,
new
locus
tentatively
named
PmSJ106
locus.
This
interval
was
composed
nucleotide-binding
leucine-rich
repeat
(NLR)
gene
cluster
containing
19
NLR
genes.
Five
NLRs
tandem
duplicated
genes,
and
one
them
(a
coiled
coil
domain–nucleotide
binding
site–leucine-rich
(CC-NBS-LRR;
CNL)
type
gene,
TaRGA5-like)
expressed
69–836-fold
compared
with
susceptible
control.
The
genome
DNA
cDNA
sequences
TaRGA5-like
amplified
SJ106,
contain
several
nucleotide
polymorphisms
LRR
regions
individuals
Chinese
Spring.
Overexpression
significantly
increased
receptor
Jinqiang5.
However,
Virus
induced
silence
(VIGS)
resulted
only
small
decrease
resistance,
presumably
compensated
by
other
results
suggested
confers
partial
SJ106.
As
member
locus,
functioned
together
improve
mildew.
variety
would
become
novel
potentially
valuable
germplasm
for
resistance.
Plant Communications,
Год журнала:
2023,
Номер
5(1), С. 100646 - 100646
Опубликована: Июль 6, 2023
Gene
cloning
in
repeat-rich
polyploid
genomes
remains
challenging.
Here
we
describe
a
strategy
for
overcoming
major
bottlenecks
the
of
powdery
mildew
(Pm)
resistance
gene
(R-gene)
Pm69
derived
from
tetraploid
wild
emmer
wheat
(WEW).
A
conventional
positional
approach
was
not
effective
due
to
suppressed
recombination.
Chromosome
sorting
compromised
by
insufficient
purity.
physical
map,
constructed
assembling
Oxford
Nanopore
Technology
(ONT)
long-read
genome
sequences,
revealed
rapidly
evolving
nucleotide-binding
leucine-rich
repeat
(NLR)
R-gene
cluster
with
structural
variations.
single
candidate
NLR
identified
anchoring
RNASeq
reads
susceptible
mutants
ONT
contigs
and
validated
virus-induced
silencing.
is
likely
newly
evolved
NLR,
which
discovered
only
one
location
across
WEW
distribution
range
Israel.
successfully
introgressed
into
cultivated
wheat,
diagnostic
molecular
marker
used
accelerate
its
deployment
pyramiding
other
R-genes.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Апрель 10, 2024
Abstract
Crop
wild
relatives
offer
natural
variations
of
disease
resistance
for
crop
improvement.
Here,
we
report
the
isolation
broad-spectrum
powdery
mildew
gene
Pm36
,
originated
from
emmer
wheat,
that
encodes
a
tandem
kinase
with
transmembrane
domain
(WTK7-TM)
through
combination
map-based
cloning,
PacBio
SMRT
long-read
genome
sequencing,
mutagenesis,
and
transformation.
Mutagenesis
assay
reveals
two
domains
WTK7-TM
are
critical
function.
Consistently,
in
vitro
phosphorylation
shows
indispensable
activity
WTK7-TM.
Haplotype
analysis
uncovers
is
an
orphan
only
present
few
indicating
its
single
ancient
origin
potential
contribution
to
current
wheat
pool.
Overall,
our
findings
not
provide
great
breeding
but
also
sheds
light
into
mechanism
underlying
resistance.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Март 19, 2024
Abstract
Wheat
powdery
mildew
is
one
of
the
most
destructive
diseases
threatening
global
wheat
production.
The
wild
relatives
constitute
rich
sources
diversity
for
resistance.
Here,
we
report
map-based
cloning
resistance
gene
Pm13
from
species
Aegilops
longissima
.
encodes
a
mixed
lineage
kinase
domain-like
(MLKL)
protein
that
contains
an
N-terminal-domain
MLKL
(MLKL_NTD)
domain
in
its
N-terminus
and
C-terminal
serine/threonine
(STK)
domain.
function
validated
by
mutagenesis,
silencing,
transgenic
assay,
allelic
association
analyses.
development
introgression
lines
with
significantly
reduced
chromosome
segments
Ae.
encompassing
enables
widespread
deployment
this
into
cultivars.
may
provide
valuable
insights
molecular
mechanisms
underlying
-mediated
highlight
important
roles
fusion
proteins
(KFPs)
immunity.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июнь 5, 2024
Abstract
Powdery
mildew
is
a
devastating
disease
that
affects
wheat
yield
and
quality.
Wheat
wild
relatives
represent
valuable
sources
of
resistance
genes.
Cloning
characterization
these
genes
will
facilitate
their
incorporation
into
breeding
programs.
Here,
we
report
the
cloning
Pm57
,
powdery
gene
from
Aegilops
searsii
.
It
encodes
tandem
kinase
protein
with
putative
kinase-pseudokinase
domains
followed
by
von
Willebrand
factor
A
domain
(WTK-vWA),
being
ortholog
Lr9
mediates
leaf
rust
resistance.
The
function
validated
via
independent
mutants,
silencing,
transgenic
assays.
Stable
lines
introgression
exhibit
high
levels
all-stage
to
diverse
isolates
Bgt
fungus,
no
negative
impacts
on
agronomic
parameters
are
observed
in
our
experimental
set-up.
Our
findings
highlight
emerging
role
fusion
proteins
plant
provide
for
breeding.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Янв. 13, 2024
Abstract
Powdery
mildew
poses
a
significant
threat
to
wheat
crops
worldwide,
emphasizing
the
need
for
durable
disease
control
strategies.
The
wheat-
Dasypyrum
villosum
T5AL·5
V#4
S
and
T5DL·5
translocation
lines
carrying
powdery
resistant
gene
Pm55
shows
developmental-stage
tissue-specific
resistance,
whereas
V#5
line
Pm5V
confers
resistance
at
all
stages.
Here,
we
clone
,
reveal
that
they
are
allelic
renamed
as
Pm55a
Pm55b
respectively.
two
alleles
encode
coiled-coil,
nucleotide-binding
site-leucine-rich
repeat
(CNL)
proteins,
conferring
broad-spectrum
mildew.
However,
interact
differently
with
linked
inhibitor
gene,
SuPm55
cause
different
Notably,
unrelated
CNL
inactivation
of
significantly
reduces
plant
fitness.
Combining
/
in
does
not
result
allele
suppression
or
yield
penalty.
Our
results
provide
only
insights
into
wheat,
but
also
strategy
breeding
resistance.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 2, 2024
Many
disease
resistance
genes
have
been
introgressed
into
wheat
from
its
wild
relatives.
However,
reduced
recombination
within
the
segments
hinders
cloning
of
genes.
Here,
we
cloned
powdery
mildew
gene
Pm13,
which
is
Aegilops
longissima,
using
a
method
that
combines
physical
mapping
with
radiation-induced
chromosomal
aberrations
and
transcriptome
sequencing
analysis
ethyl
methanesulfonate
(EMS)-induced
loss-of-function
mutants.
Pm13
encodes
kinase
fusion
protein,
designated
MLKL-K,
an
N-terminal
domain
mixed
lineage
domain-like
protein
(MLKL_NTD
domain)
C-terminal
serine/threonine
bridged
by
brace.
The
function
validated
through
transient
stable
transgenic
complementation
assays.
Transient
over-expression
analyses
in
Nicotiana
benthamiana
leaves
protoplasts
reveal
fragment
Brace-Kinase122-476
MLKL-K
capable
inducing
cell
death,
dependent
on
functional
three
α-helices
brace
region
close
to
N-terminus
domain.
was
longissima.
authors
report
it
MLKL_NTD
domain-containing
kinase.
Plant Biotechnology Journal,
Год журнала:
2023,
Номер
22(1), С. 66 - 81
Опубликована: Авг. 24, 2023
Summary
Rye
(
Secale
cereale
),
a
valuable
relative
of
wheat,
contains
abundant
powdery
mildew
resistance
Pm
)
genes.
Using
physical
mapping,
transcriptome
sequencing,
barley
stripe
mosaic
virus‐induced
gene
silencing,
ethyl
methane
sulfonate
mutagenesis,
and
stable
transformation,
we
isolated
validated
two
coiled‐coil,
nucleotide‐binding
site
leucine‐rich
repeat
(CC‐NBS‐LRR)
alleles,
PmTR1
PmTR3
,
located
on
rye
chromosome
6RS
from
different
triticale
lines.
confers
age‐related
starting
the
three‐leaf
stage,
whereas
its
allele,
typical
all‐stage
resistance,
which
may
be
associated
with
their
differential
expression
patterns.
Overexpression
in
Nicotiana
benthamiana
showed
that
CC,
CC‐NBS,
CC‐LRR
fragments
PMTR1
induce
cell
death,
PMTR3
CC
full‐length
perform
this
function.
Luciferase
complementation
imaging
pull‐down
assays
revealed
distinct
interaction
activities
between
NBS
fragments.
Our
study
elucidates
novel
rye‐derived
genes
derivative
germplasm
resources
provides
insights
into
mechanism
can
aid
improvement
against
wheat
mildew.