Genetic Basis Identification of a NLR Gene, TaRGA5-like, That Confers Partial Powdery Mildew Resistance in Wheat SJ106 DOI Open Access
Xiaoying Liu,

Chenxiao Yang,

Siqi Wu

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(12), С. 6603 - 6603

Опубликована: Июнь 15, 2024

Wheat powdery mildew is an important fungal disease that seriously jeopardizes wheat production, which poses a serious threat to food safety. SJ106 high-quality, disease-resistant spring variety; this resistance derived from Wheat-wheatgrass 33. In study, the genes in were located at end of chromosome 6DS, new locus tentatively named PmSJ106 locus. This interval was composed nucleotide-binding leucine-rich repeat (NLR) gene cluster containing 19 NLR genes. Five NLRs tandem duplicated genes, and one them (a coiled coil domain–nucleotide binding site–leucine-rich (CC-NBS-LRR; CNL) type gene, TaRGA5-like) expressed 69–836-fold compared with susceptible control. The genome DNA cDNA sequences TaRGA5-like amplified SJ106, contain several nucleotide polymorphisms LRR regions individuals Chinese Spring. Overexpression significantly increased receptor Jinqiang5. However, Virus induced silence (VIGS) resulted only small decrease resistance, presumably compensated by other results suggested confers partial SJ106. As member locus, functioned together improve mildew. variety would become novel potentially valuable germplasm for resistance.

Язык: Английский

Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69 DOI Creative Commons
Yinghui Li, Zhen-Zhen Wei, Hanan Sela

и другие.

Plant Communications, Год журнала: 2023, Номер 5(1), С. 100646 - 100646

Опубликована: Июль 6, 2023

Gene cloning in repeat-rich polyploid genomes remains challenging. Here we describe a strategy for overcoming major bottlenecks the of powdery mildew (Pm) resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat (WEW). A conventional positional approach was not effective due to suppressed recombination. Chromosome sorting compromised by insufficient purity. physical map, constructed assembling Oxford Nanopore Technology (ONT) long-read genome sequences, revealed rapidly evolving nucleotide-binding leucine-rich repeat (NLR) R-gene cluster with structural variations. single candidate NLR identified anchoring RNASeq reads susceptible mutants ONT contigs and validated virus-induced silencing. is likely newly evolved NLR, which discovered only one location across WEW distribution range Israel. successfully introgressed into cultivated wheat, diagnostic molecular marker used accelerate its deployment pyramiding other R-genes.

Язык: Английский

Процитировано

58
Wheat adaptation to environmental stresses under climate change: Molecular basis and genetic improvement DOI Creative Commons
Hude Mao, Cong Jiang, Chunlei Tang

и другие.

Molecular Plant, Год журнала: 2023, Номер 16(10), С. 1564 - 1589

Опубликована: Сен. 9, 2023

Язык: Английский

Процитировано

48
A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew DOI Creative Commons
Miaomiao Li, Huaizhi Zhang,

Huixin Xiao

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 10, 2024

Abstract Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation broad-spectrum powdery mildew gene Pm36 , originated from emmer wheat, that encodes a tandem kinase with transmembrane domain (WTK7-TM) through combination map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals two domains WTK7-TM are critical function. Consistently, in vitro phosphorylation shows indispensable activity WTK7-TM. Haplotype analysis uncovers is an orphan only present few indicating its single ancient origin potential contribution to current wheat pool. Overall, our findings not provide great breeding but also sheds light into mechanism underlying resistance.

Язык: Английский

Процитировано

26
Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein DOI Creative Commons
Huanhuan Li,

Wenqiang Men,

Chao Ma

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 19, 2024

Abstract Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives constitute rich sources diversity for resistance. Here, we report map-based cloning resistance gene Pm13 from species Aegilops longissima . encodes a mixed lineage kinase domain-like (MLKL) protein that contains an N-terminal-domain MLKL (MLKL_NTD) domain in its N-terminus and C-terminal serine/threonine (STK) domain. function validated by mutagenesis, silencing, transgenic assay, allelic association analyses. development introgression lines with significantly reduced chromosome segments Ae. encompassing enables widespread deployment this into cultivars. may provide valuable insights molecular mechanisms underlying -mediated highlight important roles fusion proteins (KFPs) immunity.

Язык: Английский

Процитировано

25
Pm57 from Aegilops searsii encodes a tandem kinase protein and confers wheat powdery mildew resistance DOI Creative Commons
Yue Zhao,

Zhenjie Dong,

Jingnan Miao

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июнь 5, 2024

Abstract Powdery mildew is a devastating disease that affects wheat yield and quality. Wheat wild relatives represent valuable sources of resistance genes. Cloning characterization these genes will facilitate their incorporation into breeding programs. Here, we report the cloning Pm57 , powdery gene from Aegilops searsii . It encodes tandem kinase protein with putative kinase-pseudokinase domains followed by von Willebrand factor A domain (WTK-vWA), being ortholog Lr9 mediates leaf rust resistance. The function validated via independent mutants, silencing, transgenic assays. Stable lines introgression exhibit high levels all-stage to diverse isolates Bgt fungus, no negative impacts on agronomic parameters are observed in our experimental set-up. Our findings highlight emerging role fusion proteins plant provide for breeding.

Язык: Английский

Процитировано

19
Fine mapping of Pm71, a novel powdery mildew resistance gene from emmer wheat DOI Creative Commons
Jiadong Zhang, Hong Yang, Guohao Han

и другие.

The Crop Journal, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

4
Fighting wheat powdery mildew: from genes to fields DOI
Bo Wang, Ting Meng,

Bei Xiao

и другие.

Theoretical and Applied Genetics, Год журнала: 2023, Номер 136(9)

Опубликована: Авг. 22, 2023

Язык: Английский

Процитировано

36
Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance DOI Creative Commons

Chuntian Lu,

Jie Du,

Heyu Chen

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Янв. 13, 2024

Abstract Powdery mildew poses a significant threat to wheat crops worldwide, emphasizing the need for durable disease control strategies. The wheat- Dasypyrum villosum T5AL·5 V#4 S and T5DL·5 translocation lines carrying powdery resistant gene Pm55 shows developmental-stage tissue-specific resistance, whereas V#5 line Pm5V confers resistance at all stages. Here, we clone , reveal that they are allelic renamed as Pm55a Pm55b respectively. two alleles encode coiled-coil, nucleotide-binding site-leucine-rich repeat (CNL) proteins, conferring broad-spectrum mildew. However, interact differently with linked inhibitor gene, SuPm55 cause different Notably, unrelated CNL inactivation of significantly reduces plant fitness. Combining / in does not result allele suppression or yield penalty. Our results provide only insights into wheat, but also strategy breeding resistance.

Язык: Английский

Процитировано

17
A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew DOI Creative Commons
Huagang He, Zhaozhao Chen,

Renchun Fan

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 2, 2024

Many disease resistance genes have been introgressed into wheat from its wild relatives. However, reduced recombination within the segments hinders cloning of genes. Here, we cloned powdery mildew gene Pm13, which is Aegilops longissima, using a method that combines physical mapping with radiation-induced chromosomal aberrations and transcriptome sequencing analysis ethyl methanesulfonate (EMS)-induced loss-of-function mutants. Pm13 encodes kinase fusion protein, designated MLKL-K, an N-terminal domain mixed lineage domain-like protein (MLKL_NTD domain) C-terminal serine/threonine bridged by brace. The function validated through transient stable transgenic complementation assays. Transient over-expression analyses in Nicotiana benthamiana leaves protoplasts reveal fragment Brace-Kinase122-476 MLKL-K capable inducing cell death, dependent on functional three α-helices brace region close to N-terminus domain. was longissima. authors report it MLKL_NTD domain-containing kinase.

Язык: Английский

Процитировано

10
Two functional CC‐NBS‐LRR proteins from rye chromosome 6RS confer differential age‐related powdery mildew resistance to wheat DOI Creative Commons
Guohao Han, Liu Hong, Shanying Zhu

и другие.

Plant Biotechnology Journal, Год журнала: 2023, Номер 22(1), С. 66 - 81

Опубликована: Авг. 24, 2023

Summary Rye ( Secale cereale ), a valuable relative of wheat, contains abundant powdery mildew resistance Pm ) genes. Using physical mapping, transcriptome sequencing, barley stripe mosaic virus‐induced gene silencing, ethyl methane sulfonate mutagenesis, and stable transformation, we isolated validated two coiled‐coil, nucleotide‐binding site leucine‐rich repeat (CC‐NBS‐LRR) alleles, PmTR1 PmTR3 , located on rye chromosome 6RS from different triticale lines. confers age‐related starting the three‐leaf stage, whereas its allele, typical all‐stage resistance, which may be associated with their differential expression patterns. Overexpression in Nicotiana benthamiana showed that CC, CC‐NBS, CC‐LRR fragments PMTR1 induce cell death, PMTR3 CC full‐length perform this function. Luciferase complementation imaging pull‐down assays revealed distinct interaction activities between NBS fragments. Our study elucidates novel rye‐derived genes derivative germplasm resources provides insights into mechanism can aid improvement against wheat mildew.

Язык: Английский

Процитировано

18