Interplay of Endoplasmic Reticulum Stress, Inflammation, Apoptosis, and Oxidative Stress in Corticosteroid-Induced Anxiety and Depression: Exploring Therapeutic Potential of Hydrogen Sulfide and Sertraline

ACS Chemical Neuroscience, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Prolonged exposure to corticosteroids (CORTs) triggers depression and anxiety symptoms either endogenously or exogenously via stimulating endoplasmic reticulum stress (ERS). The study assessed the therapeutic implications of hydrogen sulfide (H2S) versus sertraline (SERT) in alleviating induced by CORTs through modulation ERS its inflammatory, oxidative, apoptotic consequences. Rats were subdivided into four groups: control, CORT (20 mg/kg), NaHS (100 μmol/kg), SERT (10 mg/kg) for 21 days. Behavioral histological examinations cerebral cortex performed. levels CHOP, GADD34, EIF2AK3, GRP78, caspase 3, miR-146a analyzed using qRT-PCR. CORTs, serotonin, BDNF, TNF-α, BCL2, NRF2, ATF4 measured ELISA, whereas those IL-1β BAX immunohistochemical techniques. Total phosphorylated PERK western blotting, GSH MDA a colorimetric assay. In present study, upregulated gene expression Caspase it downregulated that miR-146a. GSH, NRF2 decreased, ATF4, IL-1β, BAX, elevated. On contrary, reversed all above-mentioned changes. H2S shows promise counteracting targeting cascades, mitigating inflammation, oxidative insults, apoptosis cortex. elicits neuroprotective effects miR-146a-3p/GRP78/CHOP/PERK/ATF4/GADD34 signaling pathway regulating markers BAX/BCL2 inflammatory TNF-α and/IL-1β. Compared with SERT, exhibited superior anxiolytic antidepressive effects.

Язык: Английский

The interplay of TapSAKI and NEAT-1 as potential modulators in gentamicin-induced acute kidney injury via orchestrating miR-22-3p/TLR4/MyD88/NF-қB/IL-1 β milieu: Novel therapeutic approach of Betanin

International Immunopharmacology, Год журнала: 2024, Номер 143, С. 113577 - 113577

Опубликована: Ноя. 15, 2024

Язык: Английский