Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Июнь 4, 2025
Although
there
is
great
promise
in
cascaded
DNA
nanoamplifiers
for
fluorescence
imaging,
several
key
hurdles
(e.g.,
insufficient
sensitivity,
suboptimal
reaction
kinetics,
and
unsatisfactory
detection
precision)
should
be
further
addressed.
Herein,
we
construct
a
concentration-tunable
catalytic
strand-driven
fast
self-reinforcing
circular
nanoamplifier
guided
by
the
precise
activation
of
near-infrared
(NIR)
light.
An
exogenous
strand
first
introduced
into
three-round
amplified
route,
transitioning
from
hybridization
chain
(HCR)
to
hairpin
assembly
(CHA)
then
back
HCR-CHA,
thereby
fastly
driving
more
robust
exponential
signal
enhancement.
Next,
unit
inserted
with
facile
photocleavable
coupler,
which
biosensing
course
can
precisely
activated
365
nm
ultraviolet
upconverting
luminescence
converted
NIR
light
guidance.
Upon
selecting
cancer-related
universal
marker
(microRNA-21)
as
model
target,
this
new
showcases
remarkable
competence.
Moreover,
imaging
reliably
conducted
live
cellular
environments
even
extended
animal
models,
boosting
advancement
biomedical
realm.
Abstract
DNA
circuits
show
great
potential
in
monitoring
intracellular
biomarkers
based
on
their
high
programmability,
predictability,
and
unique
signal
amplification
capabilities,
yet
face
challenges
from
uncontrollable
leakage
caused
by
the
complex
environment.
Herein,
a
demethylase‐activated
DNA‐assembly
(DAD)
circuit
is
designed
for
reliable
robust
imaging
of
cellular
microRNA,
incorporating
sequential
activation
hybridization
chain
reaction
(HCR)
amplifier
system.
The
DAD
consists
DNAzyme
module
microRNA‐recognizing
HCR
signal‐amplifying
module.
m
6
A‐modified
sequence
module,
initially
possessing
temporally
caged
substrate‐cleavage
activity,
integrated
into
probe
effectively
blocking
its
miRNA‐sensing
capacity.
In
presence
ALKBH5
demethylase,
methyl‐modifying
unit
removed,
thus
restoring
catalytic
substrate‐cleaving
activity.
This
process
exposed
previously
toehold
region
probe,
thereby
activating
sensing
miRNA.
By
leveraging
activation,
this
can
substantially
enhance
signal‐to‐background
ratio,
enabling
highly
sensitive
miRNA
detection
efficient
differentiation
cancerous
normal
cells.
Furthermore,
established
relationship
between
demethylase
enzyme
miRNA,
paving
way
investigating
more
complicate
biological
processes
intricate
signaling
pathways
within
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 25, 2025
Proteases
secreted
by
tumor
cells
have
been
employed
as
potential
biomarkers
for
the
early
assessment
of
metastasis
risk.
However,
in
situ
monitoring
their
secretion
at
single-cell
level
remains
a
challenge
due
to
low
abundance
and
diffusion
into
complex
environments.
Despite
significant
advancements
utilizing
DNA
versatile
scaffold
constructing
dynamic
sensing
systems
its
programmability
design
flexibility,
spatial
control
molecular
imaging
functions
within
living
is
particularly
challenging.
Herein,
we
developed
network
(termed
cAME)
introducing
acrylamide
copolymers
combined
with
aptamer
technology
enable
confined
detection
matrix
metalloproteinase-9
(MMP-9)
cell
invasiveness
drug
efficacy
evaluation.
The
cAME
was
established
from
combination
detecting
copolymer
(MDDC)
containing
an
MMP-9
responsive
sensor,
encapsulating
(EDC),
membrane
anchoring
module
(chol-A).
By
changes
fluorescence
signals,
real-time
activity
individual
could
be
achieved.
Using
various
cancer
lines
models,
feasibility
proposed
strategy
assessing
heterogeneity
among
different
subtypes
breast
validated.
Furthermore,
employing
two
drugs
application
value
rapid
sensitive
evaluation
demonstrated.
This
contributed
risk
while
also
providing
new
insights
evaluating
antitumor
drugs.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Июнь 4, 2025
Although
there
is
great
promise
in
cascaded
DNA
nanoamplifiers
for
fluorescence
imaging,
several
key
hurdles
(e.g.,
insufficient
sensitivity,
suboptimal
reaction
kinetics,
and
unsatisfactory
detection
precision)
should
be
further
addressed.
Herein,
we
construct
a
concentration-tunable
catalytic
strand-driven
fast
self-reinforcing
circular
nanoamplifier
guided
by
the
precise
activation
of
near-infrared
(NIR)
light.
An
exogenous
strand
first
introduced
into
three-round
amplified
route,
transitioning
from
hybridization
chain
(HCR)
to
hairpin
assembly
(CHA)
then
back
HCR-CHA,
thereby
fastly
driving
more
robust
exponential
signal
enhancement.
Next,
unit
inserted
with
facile
photocleavable
coupler,
which
biosensing
course
can
precisely
activated
365
nm
ultraviolet
upconverting
luminescence
converted
NIR
light
guidance.
Upon
selecting
cancer-related
universal
marker
(microRNA-21)
as
model
target,
this
new
showcases
remarkable
competence.
Moreover,
imaging
reliably
conducted
live
cellular
environments
even
extended
animal
models,
boosting
advancement
biomedical
realm.
