Versatile and Programmable Dual-Mode Logic Gold Nanoflares for Intracellular Correlated DNA Repair Enzymes Imaging

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117501 - 117501

Published: April 1, 2025

Language: Английский

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DNA Nanotechnology Targeting Mitochondria: From Subcellular Molecular Imaging to Tailor‐Made Therapeutics

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(36)

Published: June 14, 2024

Abstract Mitochondria, one of the most important organelles, represent a crucial subcellular target for fundamental research and biomedical applications. Despite significant advances in design DNA nanotechnologies variety bio‐applications, dearth strategies that enable mitochondria targeting molecular imaging therapy remains an outstanding challenge this field. In Minireview, we summarize recent progresses on emerging application nanotechnology mitochondria‐targeted tumor treatment. We first highlight engineering mitochondria‐localized nanosensors situ detection diverse key molecules are essential to maintain mitochondrial functions, including microRNA, enzymes, small molecules, metal ions. Then, compile developments anti‐tumor therapy, modularly designed nanodevices delivery therapeutic agents, programmed assembly interference. will place emphasis clarification chemical principles how nanobiotechnology can be various Finally, remaining challenges future directions field discussed, hoping inspire further development advanced toolkits both academic clinical regarding mitochondria.

Language: Английский

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A DNAzyme‐Based Nanohybrid for Ultrasound and Enzyme Dual‐Controlled mRNA Regulation and Combined Tumor Treatment

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Abstract Despite the significant potential of RNA‐cleaving DNAzymes for gene regulation, their application is limited by low therapeutic efficacy and lack cell‐specific control. Here, a DNAzyme‐based nanohybrid designed ultrasound (US)‐controlled, enzyme‐activatable mRNA regulation presented, enabling tumor cell‐selective combination therapy. The constructed coordination‐directed self‐assembly an enzymatically‐triggerable DNAzyme (En‐Dz) natural sonosensitizer hemoglobin (Hb). Controlled US exposure induces reactive oxygen species generation from Hb units, which not only facilitates efficient endosomal escape En‐Dz, but also promotes translocation specific enzyme nucleus to cytoplasm, thereby enhancing efficacy. Notably, enzyme‐triggered, spatiotemporally‐controlled activation En‐Dz's catalytic activity results in enhanced cancer‐cell selectivity treatment. Furthermore, enzyme‐activated sonodynamic therapy significantly enhances anti‐tumor both vitro vivo. This work highlights integrating strategy overcome current limitations regulators, providing approach precise

Language: Английский

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In‐vitro Clinical Diagnostics using RNA‐Cleaving DNAzymes

ChemBioChem, Journal Year: 2024, Volume and Issue: 25(11)

Published: April 4, 2024

Abstract Over the last three decades, significant advancements have been made in development of biosensors and bioassays that use RNA‐cleaving DNAzymes (RCDs) as molecular recognition elements. While early examples RCDs were primarily responsive to metal ions, past decade has seen numerous reported for more clinically relevant targets such bacteria, cancer cells, small metabolites, protein biomarkers. 5 years several RCD‐based also evaluated using either spiked biological matrixes or patient samples, including blood, serum, saliva, nasal mucus, sputum, urine, faeces, which is a critical step toward regulatory approval commercialization sensors. In this review, an overview methods used generate properties key utilized vitro testing first provided. Examples assays sensors test samples are then presented, highlighting assay performance different matrixes. A summary current prospects challenges diagnostic tests incorporating future directions field

Language: Английский

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Engineering two-in-one DNA nanohybrids to evaluate anti-cancer effects through activatable RNA imaging

Nano Today, Journal Year: 2024, Volume and Issue: 59, P. 102496 - 102496

Published: Sept. 14, 2024

Language: Английский

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