Fluorescent Sensors for Detecting and Imaging Metal Ions in Biological Systems: Recent Advances and Future Perspectives

Chemosensors, Год журнала: 2023, Номер 11(4), С. 226 - 226

Опубликована: Апрель 6, 2023

Metal ions play a crucial role in many biochemical processes, and when state of scarcity or surplus, they can lead to various diseases. Therefore, the development selective, sensitive, cost-effective, fast-responding sensor detect metal is critical for vitro medical diagnostics. In recent years, fluorescent sensors have been extensively investigated as potent kits effective assessment living systems due their high sensitivity, selectivity, ability perform real-time, non-invasive monitoring, versatility. This review an overview advances detection imaging biosystems from 2018 date. Specifically, we discuss application detecting essential non-essential diagnostics, cell imaging, vivo imaging. Finally, summarize remaining challenges offer future outlook on above topics.

Язык: Английский

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Photoactivatable Engineering of CRISPR/Cas9‐Inducible DNAzyme Probe for In Situ Imaging of Nuclear Zinc Ions

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(10)

Опубликована: Янв. 23, 2024

DNAzyme-based fluorescent probes for imaging metal ions in living cells have received much attention recently. However, employing situ within subcellular organelles, such as nucleus, remains a significant challenge. We developed three-stranded DNAzyme probe (TSDP) that contained 20-base-pair (20-bp) recognition site of CRISPR/Cas9, which blocks the activity. When Cas9, with its specialized nuclear localization function, forms an active complex sgRNA cell it cleaves TSDP at site, resulting formation catalytic structure. With this design, CRISPR/Cas9-inducible Zn

Язык: Английский

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Ratiometric detection of lithium ions in serum and living cells using DNAzyme-assisted hybridization chain reaction

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 410, С. 135656 - 135656

Опубликована: Март 19, 2024

Язык: Английский

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Tailoring a Minimal Self‐Replicate DNA Circuit for Highly Efficient Intracellular Imaging of microRNA

Small, Год журнала: 2023, Номер 19(17)

Опубликована: Янв. 30, 2023

Abstract Trace analyte detection in complex intracellular environment requires the development of simple yet robust self‐sufficient molecular circuits with high signal‐gain and anti‐interference features. Herein, a minimal non‐enzymatic self‐replicate DNA circuitry (SDC) system is proposed high‐signal‐gain for highly efficient biosensing living cells. It facilely engineered through self‐stacking only one elementary cascade hybridization reaction (CHR), thus encoding more economic effective amplification pathways reactants. Trigger ( T ) stimulates activation CHR producing numerous replica that reversely motivate new cycles, achieving successive self‐replication an exponentially magnified readout signal. The intrinsic circuity design self‐accelerated format SDC experimentally demonstrated theoretically simulated. With configuration low reactant complexity, amplifier enables high‐contrast accurate visualization microRNA (miRNA), ascribing to its recognition signal amplification, offering promising strategy monitoring these clinically significant analytes.

Язык: Английский

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Photoresponsive CHA-Integrated Self-Propelling 3D DNA Walking Amplifier within the Concentration Localization Effect of DNA Molecular Framework Enables Highly Efficient Fluorescence Bioimaging

Analytical Chemistry, Год журнала: 2024, Номер 96(5), С. 2142 - 2151

Опубликована: Янв. 23, 2024

While three-dimensional (3D) DNA walking amplifiers hold considerable promise in the construction of advanced DNA-based fluorescent biosensors for bioimaging, they encounter certain difficulties such as inadequate sensitivity, premature activation, need exogenous propelling forces, and low reaction rates. In this contribution, a variety profitable solutions have been explored. First, catalytic hairpin assembly (CHA)-achieved nonenzymatic isothermal nucleic acid amplification is integrated to enhance sensitivity. Subsequently, one component simply functionalized with photocleavage-bond conduct photoresponsive manner, whereby target recognition occurs only when biosensor exposed an external ultraviolet light source, overcoming activation during biodelivery. Furthermore, special self-propelling mechanism implemented by reducing biothiols MnO2 nanosheets, thereby forces that are self-supplied Mn2+-reliant DNAzyme. By carrying biosensing system molecular framework induce unique concentration localization effect, contact rate notably elevated 6 times. Following these, ultrasensitive vitro detection performance limit down 2.89 fM verified cancer-correlated microRNA biomarker (miRNA-21). Of particular importance, our multiple concepts combined 3D amplifier enables highly efficient fluorescence bioimaging live cells even bodies, exhibiting favorable application prospect disease analysis.

Язык: Английский

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A Fluorescence Light-Up 3D DNA Walker Driven and Accelerated by Endogenous Adenosine-5′-triphosphate for Sensitive and Rapid Label-Free MicroRNA Detection and Imaging in Living Cells

Analytical Chemistry, Год журнала: 2024, Номер 96(22), С. 9097 - 9103

Опубликована: Май 20, 2024

Herein, a fluorescence light-up 3D DNA walker (FLDW) was powered and accelerated by endogenous adenosine-5'-triphosphate (ATP) molecules to construct biosensor for sensitive rapid label-free detection imaging of microRNA-221 (miRNA-221) in malignant tumor cells. Impressively, ATP as the driving force accelerator FLDW could significantly accelerate operation rate FLDW, reduce likelihood errors signaling, improve sensitivity imaging. When initiated output H1-op transformed target miRNA-221, G-rich sequences S strand, anchored AuNP, were exposed form G-quadruplexes (G4s), thioflavin T (ThT) embedded G4s emitted intense realize miRNA-221. Meanwhile, specific binding ThT G4 with weak background response utilized enhance signal-to-noise ratio assay straightforwardly cost-effectively. The proposed system miRNA-221 range 1 pM 10 nM limit 0.19 employing catalytic hairpin assembly (CHA) conversion target. Furthermore, harnessing abundant present microenvironment, achieved accurate cancer This strategy provides an innovative high-speed approach biomarkers cells is expected be powerful tool bioanalysis, diagnosis, prognosis human diseases.

Язык: Английский

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Photoactivatable Engineering of CRISPR/Cas9‐Inducible DNAzyme Probe for In Situ Imaging of Nuclear Zinc Ions

Angewandte Chemie, Год журнала: 2024, Номер 136(10)

Опубликована: Янв. 23, 2024

Abstract DNAzyme‐based fluorescent probes for imaging metal ions in living cells have received much attention recently. However, employing situ within subcellular organelles, such as nucleus, remains a significant challenge. We developed three‐stranded DNAzyme probe (TSDP) that contained 20‐base‐pair (20‐bp) recognition site of CRISPR/Cas9, which blocks the activity. When Cas9, with its specialized nuclear localization function, forms an active complex sgRNA cell it cleaves TSDP at site, resulting formation catalytic structure. With this design, CRISPR/Cas9‐inducible Zn 2+ is demonstrated cells. Moreover, superiority CRISPR‐DNAzyme spatiotemporal control was by integrating photoactivation strategy and Boolean logic gate dynamic monitoring both HeLa mice. Collectively, conceptual design expands toolbox visualizing thus provides new analytical methods metal‐associated biology.

Язык: Английский

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High-fidelity intracellular imaging of multiple miRNAs via stimulus-responsive nanocarriers and catalytic hairpin assembly

Chemical Communications, Год журнала: 2024, Номер 60(17), С. 2377 - 2380

Опубликована: Янв. 1, 2024

An innovative glutathione-triggered Mn-CHP platform facilitates superior imaging of dual cancer-related miRNAs with improved cancer accuracy and specificity.

Язык: Английский

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Signal Transduction Strategies for DNAzyme-Based Sensing and Imaging of Metal Ions in Cells and in Vivo

Chemical & Biomedical Imaging, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Язык: Английский

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Copper doped carbon dots for selective determination of regulated aniline additives and safe hair dyeing

Analytica Chimica Acta, Год журнала: 2025, Номер unknown, С. 344071 - 344071

Опубликована: Апрель 1, 2025

Язык: Английский

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