Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 237, P. 115501 - 115501
Published: June 26, 2023
Language: Английский
Chemosensors, Journal Year: 2023, Volume and Issue: 11(4), P. 226 - 226
Published: April 6, 2023
Metal ions play a crucial role in many biochemical processes, and when state of scarcity or surplus, they can lead to various diseases. Therefore, the development selective, sensitive, cost-effective, fast-responding sensor detect metal is critical for vitro medical diagnostics. In recent years, fluorescent sensors have been extensively investigated as potent kits effective assessment living systems due their high sensitivity, selectivity, ability perform real-time, non-invasive monitoring, versatility. This review an overview advances detection imaging biosystems from 2018 date. Specifically, we discuss application detecting essential non-essential diagnostics, cell imaging, vivo imaging. Finally, summarize remaining challenges offer future outlook on above topics.
Language: Английский
Citations
34
Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(10)
Published: Jan. 23, 2024
DNAzyme-based fluorescent probes for imaging metal ions in living cells have received much attention recently. However, employing situ within subcellular organelles, such as nucleus, remains a significant challenge. We developed three-stranded DNAzyme probe (TSDP) that contained 20-base-pair (20-bp) recognition site of CRISPR/Cas9, which blocks the activity. When Cas9, with its specialized nuclear localization function, forms an active complex sgRNA cell it cleaves TSDP at site, resulting formation catalytic structure. With this design, CRISPR/Cas9-inducible Zn
Language: Английский
Citations
15
Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 410, P. 135656 - 135656
Published: March 19, 2024
Language: Английский
Citations
9
Small, Journal Year: 2023, Volume and Issue: 19(17)
Published: Jan. 30, 2023
Abstract Trace analyte detection in complex intracellular environment requires the development of simple yet robust self‐sufficient molecular circuits with high signal‐gain and anti‐interference features. Herein, a minimal non‐enzymatic self‐replicate DNA circuitry (SDC) system is proposed high‐signal‐gain for highly efficient biosensing living cells. It facilely engineered through self‐stacking only one elementary cascade hybridization reaction (CHR), thus encoding more economic effective amplification pathways reactants. Trigger ( T ) stimulates activation CHR producing numerous replica that reversely motivate new cycles, achieving successive self‐replication an exponentially magnified readout signal. The intrinsic circuity design self‐accelerated format SDC experimentally demonstrated theoretically simulated. With configuration low reactant complexity, amplifier enables high‐contrast accurate visualization microRNA (miRNA), ascribing to its recognition signal amplification, offering promising strategy monitoring these clinically significant analytes.
Language: Английский
Citations
19
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(5), P. 2142 - 2151
Published: Jan. 23, 2024
While three-dimensional (3D) DNA walking amplifiers hold considerable promise in the construction of advanced DNA-based fluorescent biosensors for bioimaging, they encounter certain difficulties such as inadequate sensitivity, premature activation, need exogenous propelling forces, and low reaction rates. In this contribution, a variety profitable solutions have been explored. First, catalytic hairpin assembly (CHA)-achieved nonenzymatic isothermal nucleic acid amplification is integrated to enhance sensitivity. Subsequently, one component simply functionalized with photocleavage-bond conduct photoresponsive manner, whereby target recognition occurs only when biosensor exposed an external ultraviolet light source, overcoming activation during biodelivery. Furthermore, special self-propelling mechanism implemented by reducing biothiols MnO2 nanosheets, thereby forces that are self-supplied Mn2+-reliant DNAzyme. By carrying biosensing system molecular framework induce unique concentration localization effect, contact rate notably elevated 6 times. Following these, ultrasensitive vitro detection performance limit down 2.89 fM verified cancer-correlated microRNA biomarker (miRNA-21). Of particular importance, our multiple concepts combined 3D amplifier enables highly efficient fluorescence bioimaging live cells even bodies, exhibiting favorable application prospect disease analysis.
Language: Английский
Citations
6
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(22), P. 9097 - 9103
Published: May 20, 2024
Herein, a fluorescence light-up 3D DNA walker (FLDW) was powered and accelerated by endogenous adenosine-5'-triphosphate (ATP) molecules to construct biosensor for sensitive rapid label-free detection imaging of microRNA-221 (miRNA-221) in malignant tumor cells. Impressively, ATP as the driving force accelerator FLDW could significantly accelerate operation rate FLDW, reduce likelihood errors signaling, improve sensitivity imaging. When initiated output H1-op transformed target miRNA-221, G-rich sequences S strand, anchored AuNP, were exposed form G-quadruplexes (G4s), thioflavin T (ThT) embedded G4s emitted intense realize miRNA-221. Meanwhile, specific binding ThT G4 with weak background response utilized enhance signal-to-noise ratio assay straightforwardly cost-effectively. The proposed system miRNA-221 range 1 pM 10 nM limit 0.19 employing catalytic hairpin assembly (CHA) conversion target. Furthermore, harnessing abundant present microenvironment, achieved accurate cancer This strategy provides an innovative high-speed approach biomarkers cells is expected be powerful tool bioanalysis, diagnosis, prognosis human diseases.
Language: Английский
Citations
6
Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(10)
Published: Jan. 23, 2024
Abstract DNAzyme‐based fluorescent probes for imaging metal ions in living cells have received much attention recently. However, employing situ within subcellular organelles, such as nucleus, remains a significant challenge. We developed three‐stranded DNAzyme probe (TSDP) that contained 20‐base‐pair (20‐bp) recognition site of CRISPR/Cas9, which blocks the activity. When Cas9, with its specialized nuclear localization function, forms an active complex sgRNA cell it cleaves TSDP at site, resulting formation catalytic structure. With this design, CRISPR/Cas9‐inducible Zn 2+ is demonstrated cells. Moreover, superiority CRISPR‐DNAzyme spatiotemporal control was by integrating photoactivation strategy and Boolean logic gate dynamic monitoring both HeLa mice. Collectively, conceptual design expands toolbox visualizing thus provides new analytical methods metal‐associated biology.
Language: Английский
Citations
4
Chemical Communications, Journal Year: 2024, Volume and Issue: 60(17), P. 2377 - 2380
Published: Jan. 1, 2024
An innovative glutathione-triggered Mn-CHP platform facilitates superior imaging of dual cancer-related miRNAs with improved cancer accuracy and specificity.
Language: Английский
Citations
4
Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown
Published: March 20, 2025
Language: Английский
Citations
0
Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 344071 - 344071
Published: April 1, 2025
Language: Английский
Citations
0