Analytical Chemistry,
Год журнала:
2024,
Номер
97(1), С. 622 - 628
Опубликована: Дек. 30, 2024
The
worldwide
spread
of
antibiotic
resistance
is
considered
to
be
one
the
major
health
threats
society.
While
developing
new
antibiotics
crucial,
there
also
a
strong
need
for
next-generation
analytical
methods
studying
physiological
state
live
bacteria
in
heterogeneous
populations
and
their
response
environmental
stress.
Here
we
report
single-cell
high-throughput
method
monitor
changes
bacterial
cell
envelope
stress
based
on
ratiometric
flow
cytometry.
We
used
sensitive
fluorescent
molecular
probe,
Nile
Red-based
solvatochromic
antimicrobial
peptide
UNR-1,
with
defined
cellular
localization
Gram-positive
Gram-negative
bacteria.
developed
robust
protocol
calculating
generalized
polarization
(GP)
fluorescence
adapted
Our
methodology
enabled
rapid
detection
perturbations
caused
by
exposure
antibiotics,
heat
shock,
other
factors.
Summary
Plasma
membrane
(PM)
nanodomains
have
emerged
as
pivotal
elements
in
the
regulation
of
plant
cellular
functions
and
signal
transduction.
These
nanoscale
regions,
enriched
specific
lipids
proteins,
behave
regulatory/signaling
hubs
spatially
temporally
coordinating
critical
functions.
In
this
review,
we
first
examine
mechanisms
underlying
formation
maintenance
PM
cells,
highlighting
roles
lipid
composition,
protein
oligomerization
interactions
with
cytoskeletal
cell
wall
components.
Then,
discuss
how
act
organizing
centers
by
mediating
protein–protein
that
orchestrate
essential
processes
such
symbiosis,
defense
against
pathogens,
ion
transport
or
hormonal
reactive
oxygen
species
(ROS)
signaling.
Finally,
introduce
concept
nanoenvironments,
where
localized
physicochemical
variations
are
generated
very
close
proximity
nanodomains,
response
to
stimuli.
After
decoding
a
dedicated
machinery
likely
vicinity
enrichment
secondary
messengers,
ROS
Ca
2+
,
would
allow
downstream
responses.
This
review
provides
insights
into
dynamic
nature
proposes
future
research
better
understand
their
contribution
intricate
signaling
networks
govern
development
stress
Analytical Chemistry,
Год журнала:
2024,
Номер
97(1), С. 622 - 628
Опубликована: Дек. 30, 2024
The
worldwide
spread
of
antibiotic
resistance
is
considered
to
be
one
the
major
health
threats
society.
While
developing
new
antibiotics
crucial,
there
also
a
strong
need
for
next-generation
analytical
methods
studying
physiological
state
live
bacteria
in
heterogeneous
populations
and
their
response
environmental
stress.
Here
we
report
single-cell
high-throughput
method
monitor
changes
bacterial
cell
envelope
stress
based
on
ratiometric
flow
cytometry.
We
used
sensitive
fluorescent
molecular
probe,
Nile
Red-based
solvatochromic
antimicrobial
peptide
UNR-1,
with
defined
cellular
localization
Gram-positive
Gram-negative
bacteria.
developed
robust
protocol
calculating
generalized
polarization
(GP)
fluorescence
adapted
Our
methodology
enabled
rapid
detection
perturbations
caused
by
exposure
antibiotics,
heat
shock,
other
factors.
