Dual-Signal Amplification Strategy Based on Catalytic Hairpin Assembly and APE1-Assisted Amplification for High-Contrast miRNA Imaging in Living Cells

Analytical Chemistry, Год журнала: 2024, Номер 96(2), С. 910 - 916

Опубликована: Янв. 3, 2024

Early tumor diagnosis is crucial to successful treatment. Earlier studies have shown that microRNA a biomarker for early diagnosis. The development of highly sensitive miRNA detection methods, especially in living cells, plays an indispensable role and treatment tumor. Although the catalytic hairpin assembly (CHA)-based analysis strategy commonly used disease diagnosis, further application CHA hindered due its low amplification efficiency recognition contrast. To address these limitations, we propose dual-signal based on APE1-assisted amplification, enabling high-contrast imaging. miR-221 was selected as target model. This has exhibited high efficiency, which could analyze 21 fM. also specificity, distinguish nontarget with single-base differences. Moreover, this method showed significant potential practical application, it successfully expression difference plasma samples normal people patients. Most importantly, level APE1 enzyme cells higher than allowing sensitively specifically image within cells. proposed been indicate fluctuations intracellular between cancer We anticipate will provide fresh insights can be powerful tool analysis.

Язык: Английский

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An enzymatically activated AND-gate DNA logic circuit for tumor cells recognition via multi-microRNAs detection

Biosensors and Bioelectronics, Год журнала: 2024, Номер 256, С. 116278 - 116278

Опубликована: Апрель 7, 2024

Язык: Английский

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An Enzymatically Activated and Catalytic Hairpin Assembly-Driven Intelligent AND-Gated DNA Network for Tumor Molecular Imaging

Analytical Chemistry, Год журнала: 2024, Номер 96(24), С. 10084 - 10091

Опубликована: Июнь 5, 2024

Due to the potential off-tumor signal leakage and limited biomarker content, there is an urgent need for stimulus-responsive amplification-based tumor molecular imaging strategies. Therefore, two tetrahedral framework DNA (tFNA-Hs), tFNA-H1AP, tFNA-H2, were rationally engineered form a polymeric tFNA network, termed intelligent in AND-gated manner. The network was designed tumor-specific by leveraging elevated expression of apurinic/apyrimidinic endonuclease 1 (APE1) cytoplasm instead normal cells high miRNA-21 cytoplasm. activation tFNA-H1AP can be achieved through specific recognition cleavage APE1, targeting site (AP site) modified within stem region hairpin (H1AP). Subsequently, facilitates hybridization activated H1AP on with 2 (H2) triggering catalytic assembly (CHA) reaction that opens at vertices bind H2 tFNA-H2 generate fluorescence signals. Upon completion hybridization, released, initiating subsequent cycle CHA reaction. achieve vivo also enables risk stratification neuroblastoma patients.

Язык: Английский

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Chemical Engineering of DNAzyme for Effective Biosensing and Gene Therapy

Small Methods, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

RNA-cleaving DNAzymes are in vitro selected functional nucleic acids with inherent catalytic activities. Due to their unique properties, such as high specificity, substrate cleavage capability, and programmability, have emerged powerful tools the fields of analytical chemistry, chemical biology, biomedicine. Nevertheless, biological applications still impeded by several challenges, structural instability, compromised activity environments lack spatiotemporal control designs, which may result false-positive signals, limited efficacy or non-specific activation associated side effects. To address these various strategies been explored regulate DNAzyme through modifications, enhancing stability, selectivity, functionality, thereby positioning them ideal candidates for applications. In this review, a comprehensive overview chemically modified is provided, discussing modification effects modifications on DNAzymes. Specific examples use biosensing gene therapy also presented discussed. Finally, current challenges field addressed offer perspectives potential direction

Язык: Английский

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Exploring Nucleic Acid Nanozymes: A New Frontier in Biosensor Development

Biosensors, Год журнала: 2025, Номер 15(3), С. 142 - 142

Опубликована: Фев. 24, 2025

With the growing interest in nucleic acids and nanozymes, acid nanozymes (NANs) have emerged as a promising alternative to traditional enzyme catalysts, combining advantages of nanomaterials, are widely applied field biosensing. This review provides comprehensive overview recent studies on NAN-based biosensors. It classifies NANs based six distinct enzymatic activities: peroxidase-like, oxidase-like, catalase-like, superoxide dismutase-like, laccase-like, glucose oxidase-like. emphasizes how catalytic activity is significantly influenced by properties explores regulatory mechanisms governing NANs. Additionally, it systematically reviews important research progress colorimetric, fluorescent, electrochemical, SERS, chemiluminescent sensors, offering insights into development NAN biosensor applications.

Язык: Английский

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Endogenous Enzyme-Powered DNA Nanomotor Operating in Living Cells for microRNA Imaging

Analytical Chemistry, Год журнала: 2023, Номер 95(40), С. 15025 - 15032

Опубликована: Сен. 28, 2023

Accurate and specific imaging of low-abundance microRNA (miRNA) in living cells is extremely important for disease diagnosis monitoring progression. DNA nanomotors have shown great potential molecules interest cells. However, inappropriate driving forces complex design operation procedures hindered their further application. Here, we proposed an endogenous enzyme-powered nanomotor (EEPDN), which employs APE1 enzyme as fuel to execute repetitive cycles motion miRNA The whole motor system constructed based on gold nanoparticles without other auxiliary additives. Due the high efficiency APE1, this EEPDN has achieved highly sensitive within 1.5 h. This strategy was also successfully used differentiate expression between tumor normal cells, demonstrating a cell selectivity. can promote development novel expected be perfect intracellular molecular tool biological medical applications.

Язык: Английский

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Endogenous AND Logic DNA Nanomachine for Highly Specific Cancer Cell Imaging

Analytical Chemistry, Год журнала: 2024, Номер 96(18), С. 7030 - 7037

Опубликована: Апрель 24, 2024

Intracellular cancer-related biomarker imaging strategy has been used for specific identification of cancer cells, which was great importance to accurate clinical diagnosis and prognosis studies. Localized DNA circuits with improved sensitivity showed potential intracellular biomarkers imaging. However, the ability localized specifically image cells is limited by off-site signal leakage associated a single-biomarker sensing strategy. Herein, we integrated endogenous enzyme-powered logic-responsive amplifying capability construct self-assembled endogenously AND logic nanomachine (EDN) highly cell When EDN encountered cell, overexpressed repairing enzyme apurinic/apyrimidinic endonuclease 1 (APE1) miR-21 could synergistically activate circuit via cascaded toehold-mediated strand displacement (TMSD) reactions, resulting in amplified fluorescence resonance energy transfer (FRET) signal. In this strategy, both APE1 miR-21, served as two "keys" operation reduce off-tumor leakage. Such multiplied molecular recognition/activation powerful toolbox realized capture reliable biomolecules living cells.

Язык: Английский

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Endogenous Enzyme-Activated Spatial Confinement DNA Nanowire with a Tumor Cell-Specific Response for High-Precision Imaging of the Tumor/Normal Cells Boundary

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 12, 2025

Developing tumor cell-specific imaging approaches is essential for the clear delineation of margins. However, traditional suffered from low reaction kinetics as well limited specificity resulting their "always active" sensing mode, making it difficult to accurately depict boundary. To address these limitations, we developed an endogenous enzyme-activated spatial confinement DNA nanowire probe (E-SCNW) with enhanced tumor/normal cell discrimination ratio high precision cells The effect can improve kinetics, and enzyme-activation design confine fluorescence response region. Additionally, no additional delivery carriers were required during cross membrane into intracellular space. It worth noting that benefiting design, detection limit was decreased by nearly 25.6-fold 4.46-fold through using E-SCNW, indicating promising prospects in high-precision

Язык: Английский

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Endogenous Enzyme-Driven Amplified DNA Nanocage Probe for Selective and Sensitive Imaging of Mature MicroRNAs in Living Cancer Cells

Analytical Chemistry, Год журнала: 2024, Номер 96(23), С. 9453 - 9459

Опубликована: Май 31, 2024

Selective and sensitive imaging of intracellular mature microRNAs (miRNAs) is great importance for biological process study medical diagnostics. However, this goal remains challenging because the interference precursor miRNAs (pre-miRNAs) low abundance miRNAs. Herein, we develop an endogenous enzyme-driven amplified DNA nanocage probe (Acage) selective in living cells. The Acage consists a microRNA-responsive probe, fuel strand, framework with inner cavity. Benefiting from size selectivity nanocage, smaller rather than larger pre-miRNAs are allowed to enter cavity molecular recognition; thus, can significantly reduce signal pre-miRNAs. Moreover, driving force enzyme apurinic/apyrimidinic endonuclease 1 (APE1) efficient amplification, enables miRNA without additional external intervention. With these features, was successfully applied during drug treatment. We believe that strategy provides promising pathway better understanding functions processes

Язык: Английский

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An endogenous enzyme-gated DNA nanomachine for spatially controlled imaging of intracellular microRNA and in situ evaluation of drug response

Chemical Engineering Journal, Год журнала: 2025, Номер 505, С. 159310 - 159310

Опубликована: Янв. 5, 2025

Язык: Английский

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