Multivalent DNAzyme nanomachine generated by exonuclease III-assisted recycling for fabricating ultrasensitive electrochemical biosensor

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137260 - 137260

Опубликована: Янв. 1, 2025

Язык: Английский

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Programmable DNA‐Based Nanodevices for Next‐Generation Clinical and Healthcare Applications

Advanced Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Abstract Deoxyribonucleic acid (DNA) nanotechnology has brought an unparalleled set of possibilities for self‐assembled structures emerging as independent branch synthetic biology. The field science uses the molecular properties DNA to build nanoparticles and nanodevices that have potential bring breakthroughs in medical science. On one hand, their biocompatibility, precision, ease, programmability make them ideal choice drug delivery healthcare. other, lack proper biodistribution profiles, stability inside system, enzymatic cleavage, immune recognition, translational barriers are some hurdles it faces. Many recent technological advancements progress tackle these challenges, while already been used. These tools technologies need be understood studied successful transition intelligent nanostructures (DNs) healthcare applications. This review thus, highlights challenges being faced by DNs Additionally, provides overview trends using devices disease detection remission finally talks about future scope opportunities effective from bench bedside.

Язык: Английский

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Sequential Activation of DNA Sensor Enables Correlated Imaging of Dual-Enzyme Activities in Living Cells

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 20, 2025

The DNA repair system relies on the coordinated action of multiple enzymes to maintain genomic stability, with apurinic/apyrimidinic endonuclease 1 (APE1) and flap (FEN1) playing pivotal roles in long-patch base excision (LP-BER) pathway. Elevated levels APE1 FEN1 have been associated tumor progression resistance therapy, making them key biomarkers for cancer diagnosis treatment monitoring. Here, we present a sequentially activated AND-logic sensor (D-AF) correlated imaging living cells. operates through sequential process: first recognizes cleaves an apurinic site, initiating structural changes that enable cleave 5' flap, resulting restored fluorescence. We demonstrate use D-AF-based nanosensor situ activities cells monitoring enzyme dynamics during chemotherapy. This platform offers valuable tool investigating mechanisms their treatment.

Язык: Английский

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DNA Nanoframework-Confined Well-Organized Catalytic Hairpin Assembly for Highly Sensitive and Specific Detection of MiRNA

Talanta, Год журнала: 2025, Номер 290, С. 127788 - 127788

Опубликована: Фев. 21, 2025

Язык: Английский

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Design of U-shaped peptides with long-lasting antifouling efficacy: Toward a feasible electrochemical aptasensor for robust detection in human serum

Analytica Chimica Acta, Год журнала: 2024, Номер 1318, С. 342953 - 342953

Опубликована: Июль 6, 2024

Язык: Английский

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Programmable allosteric DNA nanomachine for ratiometric imaging of VEGF in tumor cells

Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 159077 - 159077

Опубликована: Дек. 1, 2024

Язык: Английский

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