Talanta, Год журнала: 2024, Номер 285, С. 127431 - 127431
Опубликована: Дек. 19, 2024
Язык: Английский
Deleted Journal, Год журнала: 2024, Номер 1(1), С. 36 - 45
Опубликована: Янв. 17, 2024
As the most complex post-translational modification, glycosylation involves covalent linking of glycan(s) to a biomolecule. The proteins, peptides, lipids, and nucleic acids results in formation diverse glycoconjugates, like glycoproteins, peptidoglycans, glycopeptides, glycolipids, lipopolysaccharides, glycoRNAs, glycoconjugates are also ubiquitous on surface extracellular vesicles (EVs), cells, bacteria, viruses. conjugated glycans integral cell recognition (e.g., adhesion homing), trafficking, immune recognition, hormone-receptor binding, protein folding stabilization, many other biological processes. Currently, glycan-conjugated objects have found widespread applications disease diagnosis exosomes, circulating tumor cells) therapy therapeutic monoclonal antibodies). Over past decades, variety methods been established for quantitative assay objects, light their significant diagnostic values, using antibodies, aptamers, or affinity peptides as ligands. Relative methods, aptamer-based electrochemical biosensors (i.e., aptasensors) attracted much attention recent years detection targets, by virtue intrinsic advantages such low cost, good portability, simple instrument, superior stability anti-interference capability, high sensitivity selectivity. This review summarizes biological, diagnostic, significance then presents progress aptasensing including lipopolysaccharides (LPSs), cells (CTCs), pathogenic microbes bacteria viruses). Moreover, challenges future prospects discussed.
Язык: Английский
Процитировано
9
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Янв. 28, 2025
Glycoproteins are of significant value to liquid biopsy human diseases. Herein, we present a universal electrochemical platform for the amplified detection glycoproteins, taking advantage glycan-matchmade multivalent decoration enzyme labels enzymatic signal amplification. Briefly, involves two steps, i.e., site-directed phenylboronic acid-coated gold nanoparticles (PBA-AuNPs) cis-diol-containing glycans glycoproteins and subsequent via affinity cross-linking between glycan moieties remaining PBA groups on PBA-AuNP cross-linkers. As matchmaking-based strategy enables each glycoprotein with multiple labels, this exhibits high sensitivity toward detection. Using alkaline phosphatase (ALP) as proof-of-concept label in combination solid-state voltammetric stripping assay enzymatically deposited metallic silver, limits at pg mL-1 level have been obtained aptamer-based thrombin prostate-specific antigen. Overall, work illustrates an efficient versatile ultralow concentration levels, holding desirable advantages simplicity cost-effectiveness over sandwich enzyme-linked immunosorbent assays.
Язык: Английский
Процитировано
1
Deleted Journal, Год журнала: 2024, Номер 2(2)
Опубликована: Май 1, 2024
Abstract Considering the continuous advances in synthesis and clinical applications of monoclonal antibodies (mAbs), precision therapies that employ mAbs are essential. In recent years, extensive efforts have been invested developing novel strategies or technologies for detection mAbs. Given availability advanced biosensing materials, various assemblies multifunctional materials can be prepared by intelligent design when evaluating targets This article provides an overview functional mAb detection. Subsequently, we present approaches which receptors combined with to construct stimulus‐responsive analytical platforms evaluate contents activities biological systems, enabling real‐time monitoring diagnosis could facilitate administration during treatment. Furthermore, review examines implications real‐world contexts; it also discusses ongoing challenges future prospects.
Язык: Английский
Процитировано
7
Microchemical Journal, Год журнала: 2024, Номер 200, С. 110372 - 110372
Опубликована: Март 23, 2024
Язык: Английский
Процитировано
6
Sensors, Год журнала: 2025, Номер 25(7), С. 2064 - 2064
Опубликована: Март 26, 2025
Proteins represent a significant portion of the global therapeutics market, surpassing hundreds billions dollars annually. Among various post-translational modifications, glycosylation plays crucial role in influencing protein structure, stability, and function. This modification is especially important biotherapeutics, where precise characterization glycans vital for ensuring product efficacy safety. Although mass spectrometry-based techniques have become essential tools glycomic analysis due to their high sensitivity resolution, complexity lengthy processing times limit practical application. In contrast, electrochemical methods provide rapid, cost-effective, sensitive alternative assessment, enabling real-time glycan structures on biotherapeutic proteins. These techniques, often used conjunction with complementary methods, offer valuable insights into profiles both isolated glycoproteins intact cells. review examines latest advancements biosensors analysis, highlighting potential enhancing biotherapeutics advancing field precision medicine.
Язык: Английский
Процитировано
0
Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137847 - 137847
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Talanta, Год журнала: 2024, Номер 274, С. 125990 - 125990
Опубликована: Март 25, 2024
Язык: Английский
Процитировано
3
The Analyst, Год журнала: 2024, Номер 149(10), С. 2855 - 2863
Опубликована: Янв. 1, 2024
For the first time, a novel strategy for fabrication of maintenance-free and contamination-free polymeric membrane potentiometric sensors is proposed.
Язык: Английский
Процитировано
3
Analytical Chemistry, Год журнала: 2024, Номер 96(22), С. 9278 - 9284
Опубликована: Май 20, 2024
Antibody pharmaceuticals have become the most popular immunotherapeutic drugs and are often administered with low serum drug dosages. Hence, development of a highly sensitive method for quantitative assay antibody levels is great importance to individualized therapy. On basis dual signal amplification by glycan-initiated site-directed electrochemical grafting polymer chains (glyGPC), we report herein novel strategy amplified detection pharmaceuticals. The target interest was affinity captured DNA aptamer ligand, then glycans were decorated alkyl halide initiators (AHIs) via boronate cross-linking, followed ferrocenyl from through electrochemically controlled atom transfer radical polymerization (eATRP). As can be multiple AHIs grafted composed tens hundreds electroactive tags, glyGPC-based permits dually In presence trastuzumab (Herceptin) as target, achieved limit 71.5 pg/mL. Moreover, developed selective, results in human satisfactory. Owing its uncomplicated operation cost-effectiveness, shows promise
Язык: Английский
Процитировано
2
Biosensors and Bioelectronics, Год журнала: 2024, Номер 271, С. 116988 - 116988
Опубликована: Ноя. 23, 2024
Язык: Английский
Процитировано
2