Deleted Journal,
Год журнала:
2024,
Номер
1(3), С. 199 - 204
Опубликована: Авг. 6, 2024
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV2)
has
been
identified
as
the
causative
agent
for
COVID-19
pandemic.
Herein,
we
report
development
of
a
point-of-care
diagnostic
(POC)
test
strip
rapid
and
specific
diagnosis
surface
spike
protein
(S-protein)
SARS-CoV2
virus,
target
analyte
virus
diagnosis.
The
detects
changes
in
physicochemical
properties
gold
nanoparticles
with
utility
DNA
aptamer
that
is
specifically
selected
binding
to
receptor-binding
domain
(RBD)
S-protein
SARS-CoV-2.
Structural
enables
aggregation/disaggregation
absence/presence
analyte,
respectively.
Lateral
flow
assays
were
utilized
visualize
results
stratify
positive
samples
(containing
viral
S-protein)
from
negative
(blank)
high
specificity
sensitivity
(1–10
nM).
proposed
sensing
technology
was
successfully
developed
quantification
S-protein;
however,
it
can
be
further
adapted
detect
variants,
DNA/RNA,
other
targets,
enabling
POC
devices
multiplexing
assays.
Point-of-care
(POC)
nanosensors
with
high
screening
efficiency
show
promise
for
user-friendly
manipulation
in
the
ever-increasing
on-site
analysis
demand
illness
diagnosis,
environmental
monitoring,
and
food
safety.
Currently,
inspired
by
merits
of
integrating
advanced
nanomaterials,
molecular
biology,
machine
learning,
artificial
intelligence,
lateral
flow
immunoassay
(LFIA)-based
POC
have
been
devoted
to
satisfying
commercial
demands
terms
sensitivity,
specificity,
practicality.
Herein,
we
examine
use
multidimensional
enhanced
LFIA
various
fields
over
past
two
decades,
focusing
on
introducing
nanomaterials
improve
acquisition
capability
small
order
magnitude
targets
through
engineering
transformations
emphasizing
interdomain
fusion
collaboratively
address
inherent
challenges
current
applications,
such
as
multiplexing,
development
detectors
quantitative
analysis,
more
practical
sensitivity
enhancement.
Specifically,
this
comprehensive
review
encompasses
latest
advances
comprehending
an
alternative
signal
transduction
pattern,
aiming
achieve
rapid,
ultrasensitive,
"sample-to-answer"
available
options
progressive
applications
nanosensors.
In
summary,
cross-collaboration
disciplines,
has
potential
break
barriers
toward
commercialization
laboratory-level
nanosensors,
thus
leading
emergence
next
generation
LFIA.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 18, 2025
Lateral
flow
immunoassays
(LFIA)
are
widely
recognized
as
cost-effective
point-of-care
diagnostic
tools
(POCT)
for
infectious
disease
diagnosis.
Despite
their
widespread
use,
traditional
colorimetric
LFIAs,
which
rely
on
gold
nanospheres
(GNP),
constrained
by
a
limited
sensitivity.
To
overcome
this
challenge,
we
have
engineered
nanocages
(GNCs)
with
optimized
core-to-shell
morphologies,
achieving
significant
amplification
of
both
and
photothermal
LFIA
readout
signals.
The
distinctive
morphology
GNCs,
featuring
adjustable
gap
thicknesses,
enables
fine-tuning
the
localized
surface
plasmon
resonance
(LSPR)
peak
across
broad
spectral
range
from
600
to
1200
nm.
Among
GNC
morphologies
evaluated,
(GNC-4),
characterized
its
larger
size
maximal
thickness,
exhibited
superior
color
brightness
enhanced
efficiency
compared
other
GNP.
performance
GNC-4
enabled
detection
influenza
A
(H1N1),
used
model
analyte,
limit
(LOD)
1.8
ng/mL
via
analysis
1.51
pg/mL
using
LFIA.
Compared
GNP-based
detection,
sensitivity
GNC-4-based
was
7-fold,
while
showed
an
improvement
over
8000-fold.
By
incorporating
portable
smartphone-based
platform,
our
dual-modal
exhibits
high
sensitivity,
practicality
in
detecting
H1N1
spiked
saliva
samples,
long-term
stability
five
months,
making
it
promising
tool
potential
diagnosing
pathogens.
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 5, 2024
Abstract
Lateral
flow
immunoassays
(LFIA)
are
widely
used
for
the
cost‐effective
and
rapid
detection
of
diverse
analytes.
However,
traditional
LFIA
suffers
from
difficulties
in
providing
quantitative
results
has
low
sensitivity.
Herein,
is
combined
with
electrochemiluminescence
(ECL),
a
leading
transduction
technique
high
sensitivity
wide
dynamic
range,
to
achieve
highly
sensitive
severe
acute
respiratory
syndrome
coronavirus
nucleocapsid
protein
(SARS‐CoV‐2
N
protein).
Ruthenium(II)‐based
complexes
synthesized
loaded
into
dendritic
mesoporous
silica
nanospheres
(PEI‐Ru/dSiO
2
),
which
possessed
central‐radial
pore
channels
served
as
tags
ECL‐LFIA.
The
electrodes
fabricated
on
nitrocellulose
(NC)
membrane,
simplifies
structure
PEI‐Ru/dSiO
captured
electrode
surface
via
sandwich
immunoreaction,
enhances
ECL
signal
by
decreasing
distance
between
surface.
Using
2,2‐bis(hydroxymethyl)‐2,2′,2′'‐nitrilotriethanol
(BIS‐TRIS)
coreactant,
ECL‐LFIA
detecting
SARS‐CoV‐2
protein,
linear
range
1–10
4
ng
mL
−1
limit
(LOD)
0.52
.
can
also
be
detect
analytes
complex
matrices.
These
demonstrate
that
prepared
great
potential
point‐of‐care
testing
platform
disease
biomarkers.
Biosensors,
Год журнала:
2024,
Номер
14(4), С. 197 - 197
Опубликована: Апрель 17, 2024
Controlling
the
progression
of
contagious
diseases
is
crucial
for
public
health
management,
emphasizing
importance
early
viral
infection
diagnosis.
In
response,
lateral
flow
assays
(LFAs)
have
been
successfully
utilized
in
point-of-care
(POC)
testing,
emerging
as
a
viable
alternative
to
more
traditional
diagnostic
methods.
Recent
advancements
virus
detection
primarily
leveraged
methods
such
reverse
transcription–polymerase
chain
reaction
(RT-PCR),
transcription–loop-mediated
isothermal
amplification
(RT-LAMP),
and
enzyme-linked
immunosorbent
assay
(ELISA).
Despite
their
proven
effectiveness,
these
conventional
techniques
are
often
expensive,
require
specialized
expertise,
consume
significant
amount
time.
contrast,
LFAs
utilize
nanomaterial-based
optical
sensing
technologies,
including
colorimetric,
fluorescence,
surface-enhanced
Raman
scattering
(SERS),
offering
quick,
straightforward
analyses
with
minimal
training
infrastructure
requirements
detecting
proteins
biological
samples.
This
review
describes
composition
mechanism
recent
protein
detection,
categorizing
them
into
fluorescent,
SERS-based
techniques.
progress,
developing
simple,
stable,
highly
sensitive,
selective
LFA
system
remains
formidable
challenge.
Nevertheless,
an
advanced
promises
not
only
enhance
clinical
diagnostics
but
also
extend
its
utility
environmental
monitoring
beyond,
demonstrating
potential
revolutionize
both
healthcare
safety.
Molecules,
Год журнала:
2024,
Номер
29(19), С. 4551 - 4551
Опубликована: Сен. 25, 2024
Immunoassays
have
been
widely
used
for
the
determination
of
various
analytes
in
fields
disease
diagnosis,
food
safety,
and
environmental
monitoring.
Dual-signal
immunoassays
are
now
advanced
integrated
detection
technologies
with
excellent
self-correction
self-validation
capabilities.
In
this
work,
we
summarize
recent
advances
development
optical
electrochemical
dual-signal
immunoassays,
including
colorimetric,
fluorescence,
surface-enhanced
Raman
spectroscopy
(SERS),
electrochemical,
electrochemiluminescence,
photoelectrochemical
methods.
This
review
particularly
emphasizes
working
principle
diverse
utilization
dual-functional
molecules
nanomaterials.
It
also
outlines
challenges
prospects
future
research
on
immunoassays.
ACS Sensors,
Год журнала:
2024,
Номер
9(10), С. 5293 - 5301
Опубликована: Окт. 2, 2024
The
early
diagnosis
of
cancer
in
a
point-of-need
manner
is
great
significance,
yet
it
remains
challenging
to
achieve
the
necessary
sensitivity
and
speed.
Traditional
lateral
flow
immunoassay
(LFIA)
methods
are
limited
accuracy
quantification,
restricting
their
suitability
for
home-based
applications.
Thus,
we
explored
new
user-friendly
electrochemical
LFIA
(e-LFIA)
test
strip
detect
α-fetoprotein
(AFP),
diagnostic
marker
liver
cancer.
specific
immunoprobe
utilized
this
e-LFIA
characterized
by
significant
signal
boosting,
resulted
from
loading
Ag
shell
into
gold
nanoparticle
(AuNP)-coated
dendritic
mesoporous
silica
nanoscaffold
(DMSN).
Leveraging
distinct
characteristics
anodic
stripping
high
volume-to-surface
area
ratio
DMSNs,
developed
DMSNs/AuNPs@Ag-based
capable
detecting
AFP
at
low
concentration
0.85
ng/mL
within
rapid
20
min
timespan,
both
these
values
smaller
than
those
current
clinical
testing.
Furthermore,
homemade
screen-printed
electrodes
sensing
prototype
demonstrated
versatility
reliability
device.
We
envision
that
holds
substantial
potential
household
health
monitoring.
