Nitrogen stable isotope analysis of sulfonamides by derivatization-gas chromatography-isotope ratio mass spectrometry DOI Creative Commons

Qingyuan Dou,

Aoife Canavan,

Yuhao Fu

и другие.

Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер 416(19), С. 4237 - 4247

Опубликована: Июнь 7, 2024

Abstract The continuous introduction of micropollutants into the environment through livestock farming, agricultural practices, and wastewater treatment is a major concern. Among these pollutants are synthetic sulfonamide antibiotics such as sulfamethoxazole, which not always fully degraded pose risk fostering antimicrobial resistance. It challenging to assess degradation sulfonamides with conventional concentration measurements. This study introduces compound-specific isotope analysis nitrogen ratios at natural abundances by derivatization-gas chromatography hyphenated ratio mass spectrometry (derivatization-GC-IRMS) new more precise method for tracing origin sulfonamides. Here, sulfamethoxazole was used model compound develop optimize derivatization conditions using (trimethylsilyl)diazomethane reagent. With optimized conditions, accurate reproducible δ 15 N derivatization-GC-IRMS achieved in two different laboratories limit 3 nmol on column, corresponding 0.253 µg non-derivatized SMX. Application four further sulfonamides, sulfadiazine, sulfadimethoxine, sulfadimidine, sulfathiazole, shows versatility developed method. Its benefit demonstrated first application, highlighting possibility distinguishing from suppliers pharmaceutical products. Graphical

Язык: Английский

Lack of selectivity in sample preparation ‒ An achilles heel of compound-specific isotope analysis for environmental micropollutants DOI Creative Commons
Rani Bakkour, Christopher Wabnitz, David Glöckler

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 180, С. 117908 - 117908

Опубликована: Авг. 12, 2024

The role of compound-specific isotope analysis (CSIA) in environmental research has been proven over the last few decades. Despite advances analytical methods and instrumentation, applying CSIA to low-concentration contaminants, especially at field scale, remains limited. In this perspective paper, we argue that limitation stems from underdeveloped sample preparation techniques, particularly lack required selectivity. Drawing an extensive review nearly 600 studies on (i) analyze methodologies' distribution dedication studies, discussing their connection with maturity suitable techniques. Additionally, (ii) examine general trends sorbent phase technologies, assessing adequacy meet CSIA's targeted nature applicability micropollutants. advocating for a paradigm shift, (iii) emphasize need adapt future development strategies light past current innovations.

Язык: Английский

Процитировано

3

Quartz Crystal Microbalance as a Holistic Detector for Quantifying Complex Organic Matrices during Liquid Chromatography: 1. Coupling, Characterization, and Validation DOI Creative Commons
Christopher Wabnitz,

Aoife Canavan,

Wei Chen

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(19), С. 7429 - 7435

Опубликована: Апрель 29, 2024

A matrix in highly complex samples can cause adverse effects on the trace analysis of targeted organic compounds. suitable separation target analyte(s) and before instrumental is often a vital step for which chromatographic cleanup methods remain one most frequently used strategies, particularly high-performance liquid chromatography (HPLC). The lack simple real-time detection technique that quantify entirety during this step, especially with gradient solvents, renders optimization challenging. This paper, along companion one, explores possibilities limitations quartz crystal microbalance (QCM) dry-mass sensing quantifying matrices HPLC. To end, work coupled QCM microfluidic spray dryer commercial HPLC system using flow splitter developed calibration data processing strategy. was characterized terms quantification limits, LOD = 4.3–15 mg/L LOQ 16–52 mg/L, respectively, different eluent compositions. Validation natural matter an environmental sample against offline total carbon confirmed approach's feasibility, absolute recovery 103 ± 10%. Our findings suggest could serve as valuable tool analysts routinely employing methods, offering potential benefits across various analytical fields.

Язык: Английский

Процитировано

2

Nitrogen stable isotope analysis of sulfonamides by derivatization-gas chromatography-isotope ratio mass spectrometry DOI Creative Commons

Qingyuan Dou,

Aoife Canavan,

Yuhao Fu

и другие.

Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер 416(19), С. 4237 - 4247

Опубликована: Июнь 7, 2024

Abstract The continuous introduction of micropollutants into the environment through livestock farming, agricultural practices, and wastewater treatment is a major concern. Among these pollutants are synthetic sulfonamide antibiotics such as sulfamethoxazole, which not always fully degraded pose risk fostering antimicrobial resistance. It challenging to assess degradation sulfonamides with conventional concentration measurements. This study introduces compound-specific isotope analysis nitrogen ratios at natural abundances by derivatization-gas chromatography hyphenated ratio mass spectrometry (derivatization-GC-IRMS) new more precise method for tracing origin sulfonamides. Here, sulfamethoxazole was used model compound develop optimize derivatization conditions using (trimethylsilyl)diazomethane reagent. With optimized conditions, accurate reproducible δ 15 N derivatization-GC-IRMS achieved in two different laboratories limit 3 nmol on column, corresponding 0.253 µg non-derivatized SMX. Application four further sulfonamides, sulfadiazine, sulfadimethoxine, sulfadimidine, sulfathiazole, shows versatility developed method. Its benefit demonstrated first application, highlighting possibility distinguishing from suppliers pharmaceutical products. Graphical

Язык: Английский

Процитировано

1