Sensors & Diagnostics, Год журнала: 2024, Номер 3(8), С. 1353 - 1357
Опубликована: Янв. 1, 2024
Based on DNA-based signal amplification strategies, a nicking endonuclease Nb.BbvCI powered DNA walker machine-based electrochemical aptasensor exhibited high sensitivity and good selectivity in the detection of STX.
Язык: Английский
Analytical Chemistry, Год журнала: 2024, Номер 96(31), С. 12838 - 12845
Опубликована: Июль 25, 2024
MicroRNA (miRNA) detection is a critical aspect of disease diagnosis, and recent studies indicate that miRNA-622 could be potential target for lung cancer. Herein, Cu single atoms were anchored on graphitic carbon nitride (Cu SAs@CN) as coreaction accelerator applied in luminol-H
Язык: Английский
Процитировано
6
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Окт. 18, 2024
Язык: Английский
Процитировано
5
Sensors and Actuators B Chemical, Год журнала: 2024, Номер 415, С. 136010 - 136010
Опубликована: Май 22, 2024
Язык: Английский
Процитировано
4
Analytical Chemistry, Год журнала: 2024, Номер 96(33), С. 13727 - 13733
Опубликована: Авг. 7, 2024
In this work, an ultrasensitive electrochemiluminescence (ECL) biosensor was constructed based on DNA-stabilized Au Ag nanoclusters (DNA-Au NCs) as the efficient luminophore and NPs@Ti
Язык: Английский
Процитировано
4
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Фев. 18, 2025
Herein, an electrochemiluminescence (ECL) biosensor is established for the ultrasensitive detection of microRNA (miRNA) by integrating a self-protected DNAzyme walker machine on Au nanoparticle–modified electrode. Using Let-7a miRNA as model target and introducing target-binding domain into middle catalytic core, core separated that can inhibit cleavage activity serve arch-like protective shield, resulting in walker. High-efficiency hybridization between activates machine, enabling high its substrate without requiring additional energy input. Importantly, each step results strand liberation Ru(bpy)2(mcpbpy)2+ (Ru)-labeled DNA fragment, considerably reducing ECL signal Ru. Under optimized experimental conditions, limit 51.4 aM within wide linear range 100 aM–100 pM. This proposed strategy bold innovation rapid sensitive low-abundance biomarkers, offering promising application early cancer diagnosis relevant research.
Язык: Английский
Процитировано
0
Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113297 - 113297
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Апрель 16, 2025
In this work, using Al-H3NTB-MOG with self-enhanced and aggregation-induced electrochemiluminescence (AIECL) performance as an efficient emitter, a biosensor based on Y-shaped multiregion dual-drive DNA walker was constructed for the sensitive detection of miRNA-141. Notably, 4,4',4″-nitrilotribenzoic acid (H3NTB) selected luminescent ligand ECL property co-reactive tertiary amine in its structure. Al3+ served central ion to coordinate H3NTB form three-dimensional porous gel structure, which restricted internal rotation vibration benzene molecules exhibited excellent AIECL property. More interestingly, N-2-hydroxyethylpiperazine-N'-ethane-sulfonic (HEPES) chosen system buffer, could not only stabilize test environment but also play co-reaction compensation role compensate consumption groups process, then significantly resulting better stability response. Besides, dynamic signal amplification established synergistic effect rolling cycle (RCA) process ionic cleavage at both ends nanostructure assembled by catalytic hairpin self-assembly (CHA) reaction. Specifically, two long chains abundant recognition regions were formed RCA reaction walker, simultaneously walk along predesigned tracks shear specific sites from directions, effectively improving efficiency. that way, realized miRNA-141 10 aM 1 nM range limit low 6.48 aM.
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2024, Номер 96(37), С. 15050 - 15058
Опубликована: Сен. 5, 2024
An efficient electrochemiluminescence (ECL) emitter, Ir(ppy)
Язык: Английский
Процитировано
2
Analytical Chemistry, Год журнала: 2024, Номер unknown
Опубликована: Окт. 26, 2024
Dynamic DNA nanodevices, particularly walkers, have proven to be versatile tools for target recognition, signal conversion, and amplification in biosensing. However, their ability detect low-abundance analytes complex biological samples is often compromised by limited depth severe leakage. To address these challenges, we developed a simple yet highly efficient strategy engineer self-replicating bipedal DNAzyme (SEDY) walker sensitive selective electrochemiluminescence (ECL) bioanalysis. Unlike conventional walkers that are typically constructed catalytic assembly single direction, the SEDY integrates feedback mechanism greatly enhances both selectivity sensitivity of First, assembled through target-triggered, enzyme-free, approach, minimizing risk undesired side reactions leakage simplifying reactant complexity. Furthermore, features newly exposed trigger sequences facilitate its autonomous replication, leading robust exponential products. Our experiments demonstrate can sensitively selectively acetamiprid navigating specific probes within cross-shaped orbits. The ECL biosensor offers linear detection range from 1 × 10
Язык: Английский
Процитировано
2