CRISPR/Cas14a integrated with DNA walker based on magnetic self-assembly for human papillomavirus type 16 oncoprotein E7 ultrasensitive detection
Biosensors and Bioelectronics,
Год журнала:
2025,
Номер
272, С. 117135 - 117135
Опубликована: Янв. 5, 2025
Язык: Английский
Functional Nucleic Acid Nanostructures for Mitochondrial Targeting: The Basis of Customized Treatment Strategies
Molecules,
Год журнала:
2025,
Номер
30(5), С. 1025 - 1025
Опубликована: Фев. 24, 2025
Mitochondria,
as
vital
organelles,
play
a
central
role
in
subcellular
research
and
biomedical
innovation.
Although
functional
nucleic
acid
(FNA)
nanostructures
have
witnessed
remarkable
progress
across
numerous
biological
applications,
strategies
specifically
tailored
to
target
mitochondria
for
molecular
imaging
therapeutic
interventions
remain
scarce.
This
review
delves
into
the
latest
advancements
leveraging
FNA
mitochondria-specific
cancer
therapy.
Initially,
we
explore
creation
of
FNA-based
biosensors
localized
mitochondria,
enabling
real-time
detection
visualization
critical
molecules
essential
mitochondrial
function.
Subsequently,
examine
developments
aimed
at
mitochondrial-targeted
treatments,
including
modular
nanodevices
precise
delivery
agents
programmable
disrupting
processes.
Emphasis
is
placed
on
elucidating
chemical
principles
underlying
design
mitochondrial-specific
nanotechnology
diverse
uses.
Lastly,
address
unresolved
challenges
outline
prospective
directions,
with
goal
advancing
field
encouraging
sophisticated
tools
both
academic
inquiry
clinical
applications
centered
mitochondria.
Язык: Английский
APE1-Activated and NIR-II Photothermal-Enhanced Chemodynamic Therapy Guided by Amplified Fluorescence Imaging
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 14, 2025
The
development
of
intelligent
nanotheranostic
technology
that
integrates
diagnostic
and
therapeutic
functions
holds
great
promise
for
personalized
nanomedicine.
However,
most
the
agents
exhibit
"always-on"
properties
do
not
involve
an
amplification
step,
which
may
largely
limit
imaging
contrast
restrict
efficacy.
Herein,
we
construct
a
novel
platform
(Hemin/DHPs/PDA@CuS
nanocomposite)
by
assembling
DNA
hairpin
probes
(DHPs)
hemin
on
surface
PDA@CuS
nanosheets
enables
amplified
fluorescence
activatable
chemodynamic
therapy
(CDT)
tumors.
cancer-relevant
APE1
triggers
nucleic
acid
with
DHPs
to
generate
signals
discriminating
cancer
cells
from
normal
cells.
Meanwhile,
excessive
G-quadruplex/hemin-based
DNAzyme
are
also
activated,
they
function
as
Fenton-like
catalysts
catalyze
production
highly
toxic
hydroxyl
radicals
(•OH)
CDT.
Moreover,
owing
excellent
photothermal
conversion
efficiency
in
near-infrared-II
(NIR-II)
window,
only
improves
catalytic
performance
CDT
but
furnishes
PTT.
A
remarkable
antitumor
effect
is
demonstrated
both
vitro
vivo.
Therefore,
Hemin/DHPs/PDA@CuS
nanocomposite
expected
provide
promising
avenue
precise
imaging-guided
therapy.
Язык: Английский
Engineering Multi-activator-encoded DNA Nanonet to Accelerate CRISPR-Cas12a Activation for Rapid and Sensitive Electrochemiluminescence Bioassay
Talanta,
Год журнала:
2025,
Номер
288, С. 127724 - 127724
Опубликована: Фев. 11, 2025
Язык: Английский
Sequential Activation of DNA Sensor Enables Correlated Imaging of Dual-Enzyme Activities in Living Cells
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 20, 2025
The
DNA
repair
system
relies
on
the
coordinated
action
of
multiple
enzymes
to
maintain
genomic
stability,
with
apurinic/apyrimidinic
endonuclease
1
(APE1)
and
flap
(FEN1)
playing
pivotal
roles
in
long-patch
base
excision
(LP-BER)
pathway.
Elevated
levels
APE1
FEN1
have
been
associated
tumor
progression
resistance
therapy,
making
them
key
biomarkers
for
cancer
diagnosis
treatment
monitoring.
Here,
we
present
a
sequentially
activated
AND-logic
sensor
(D-AF)
correlated
imaging
living
cells.
operates
through
sequential
process:
first
recognizes
cleaves
an
apurinic
site,
initiating
structural
changes
that
enable
cleave
5'
flap,
resulting
restored
fluorescence.
We
demonstrate
use
D-AF-based
nanosensor
situ
activities
cells
monitoring
enzyme
dynamics
during
chemotherapy.
This
platform
offers
valuable
tool
investigating
mechanisms
their
treatment.
Язык: Английский