Self-Correcting Ratiometric Fluorescence Assay for H2S Detection in Live Cells and Releasing Agents

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 422, С. 136567 - 136567

Опубликована: Сен. 4, 2024

Язык: Английский

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Dual-Lock System for High Sensitivity and Selectivity in Redox Enzyme Activation and Imaging

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Reductase expression is a potential indicator of cellular pathology. Single-detection systems for reductases have been developed, however, the development dual-detection remain largely unexplored. We rationally designed dual-lock fluorescent probe that exhibited high signal-to-noise ratio with fluorescence Off-On response exclusively simultaneous activity two reductases, NTR and hNQO1, which are overexpressed in cancer hypoxia. The system comprised naphthalimide fluorophore control mediated by PET ICT, trimethyl-locked quinone group sensitive to nitrobenzyl carbamate NTR. This study employed hypoxia model HeLa cells demonstrate our developed detected more effectively than single-detection systems. Moreover, it enabled noninvasive real-time monitoring zebrafish embryos. Consequently, probe, strategically provides only when both NQO1 active, offers novel diagnostic platform vitro vivo applications, detecting various pathological states.

Язык: Английский

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A Dual-Responsive Fluorescent Probe for Cancer Diagnostics via Simultaneous Detection of H2S and hNQO1

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137682 - 137682

Опубликована: Март 1, 2025

Язык: Английский

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A dual-reactivity-based surface-enhanced Raman spectroscopy nanosensor for the simultaneous imaging of hypochlorite and nitric oxide in living cells

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2025, Номер unknown, С. 125899 - 125899

Опубликована: Фев. 1, 2025

Язык: Английский

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Naphthalenediimide-Based Heavy-Atom-Free Nanoparticles for Enhanced Dual-Functional Type I Photodynamic and Photothermal Therapy

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137526 - 137526

Опубликована: Фев. 1, 2025

Язык: Английский

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A novel fluorescent sensor for imaging of viscosity and ClO− in plant cells and zebrafish

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2025, Номер unknown, С. 126162 - 126162

Опубликована: Апрель 1, 2025

Язык: Английский

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Off–On fluorescent benzothiazole‐fused coumarin for sensitive detection of nitroreductases and hydrogen sulfide

Bulletin of the Korean Chemical Society, Год журнала: 2024, Номер 45(8), С. 699 - 705

Опубликована: Авг. 1, 2024

Abstract Hydrogen sulfide (H 2 S) and nitroreductases (NTR) are key biological reductants, with H S acting as a signaling molecule NTR reducing nitro groups to amines in cellular metabolism. Visualizing these components, often overexpressed cancer cells or bacterial environments, is crucial for metabolic understanding. Existing fluorescent probes detect individually, but simultaneous detection methods unexplored. We designed probe 1 , which shows high fluorescence only when both overexpressed. The transformed into benzothiazole‐fused coumarin by NTR, exhibiting fluorescence, stronger signal the presence of S, making it potential tool detecting their overexpression.

Язык: Английский

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Sensitive Cancer Hypoxia Detection via a Dual-Locking Fluorescence Response System Using Two Hypoxia Indicators

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Сен. 11, 2024

Hypoxia is intricately associated with various diseases, including ischemia, vascular disorders, and cancer. Particularly in cancer cells, hypoxia promotes tumor growth, cell proliferation, migration, invasion enhances treatment resistance, making its detection crucial for diagnosis therapy. However, methods detecting are limited, often relying on single-detection systems. In this study, we developed a dual-lock-based fluorescent probe that selectively exhibits strong green fluorescence under hypoxic conditions due to simultaneous activity of nitroreductases (NTRs) hydrogen sulfide (H

Язык: Английский

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Responsive Theranostic Nanoprobe for Ratiometric Photoacoustic Monitoring of Hypochlorous Acid‐Mediated Inflammation in Cancer Photothermal Therapy

Advanced Functional Materials, Год журнала: 2024, Номер unknown

Опубликована: Окт. 23, 2024

Abstract Cancer detection and inflammation monitoring during photothermal therapy (PTT) enable timely cancer intervention precise control, advancing to address inflammation‐related tumor recurrence metastasis associated with PTT. This can be achieved through real‐time biomarker for inflammation, like hypochlorous acid (HOCl), a highly reactive oxygen species (hROS) in body elevated levels inflammation. Here, HOCl‐responsive theranostic nanoprobe is introduced, AuNRs@SiO 2 ‐CAA ratiometric photoacoustic (PA) emits PA signals at 680 820 nm, only PA680 undergoing changes the presence of HOCl, enabling HOCl imaging via recording (PA680/PA820). exhibits high selectivity sensitivity, limit 0.34 µM HOCl. In vivo, it effectively detects tumor, drives PTT, monitors PTT by sensing The successful development offers novel system diagnosis, poised enhance control.

Язык: Английский

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Rapid assessment of acetophenone using an anti-interfering triple-emission Ln3+-functionalized HOF@MOF sensor

Talanta, Год журнала: 2024, Номер 280, С. 126718 - 126718

Опубликована: Авг. 15, 2024

Язык: Английский

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