Journal of Analytical Science & Technology,
Год журнала:
2024,
Номер
15(1)
Опубликована: Ноя. 5, 2024
Abstract
The
catalytic
hairpin
assembly
(CHA)-based
miRNA
detection
methods
have
garnered
significant
attention
due
to
their
simplicity
and
acceptable
amplification
efficiency.
However,
these
necessitate
improved
sensitivity.
In
this
work,
we
present
a
colorimetric
ultrasensitive
approach
for
the
of
cancer-related
miRNAs
which
is
initiated
by
CHA-mediated
nicking
endonuclease-assisted
signals
recycling.
target
initiates
CHA
process
expose
functional
section
in
P2
probe.
This
can
activate
cascaded
recycling
cycles
produce
numerous
linker
sequences
Nt.AlwI
cleavage
two
signal
probes.
3,3’,5,5’-tetramethylbenzidine
sulfate
(TMB)/H
2
O
-based
color
reaction
induced
fixation
cDNA-HRP
on
surface
magnetic
beads,
mediated
sequence.
proposed
method
demonstrates
sensitivity
that
either
comparable
or
superior
previous
methods,
as
result
design.
Furthermore,
demonstrated
promising
potential
clinical
applications
high
selectivity
miRNA.
Consequently,
it
provides
assay
both
dependable
visual
miRNA,
has
revolutionize
early
diagnosis
cancer.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 13, 2025
An
entropy-driven
catalysis
(EDC)
strategy
is
appealing
for
amplified
bioimaging
of
microRNAs
in
living
cells;
yet,
complex
operation
procedures,
lacking
cell
selectivity,
and
insufficient
accuracy
hamper
its
further
applications.
Here,
we
introduce
an
ingenious
all-in-one
DNA
nanomachine
(termed
as
AIO-EDN),
which
can
be
triggered
by
endogenous
apurinic/apyrimidinic
endonuclease
1
(APE1)
to
achieve
tumor
cell-selective
dual-mode
imaging
microRNA.
Compared
with
the
traditional
EDC
strategy,
integrated
design
AIO-EDN
achieves
autocatalytic
signal
amplification
without
extra
fuel
strands.
Moreover,
leverages
APE1
overexpressed
cancer
cells
activate
reaction,
which,
however,
exerts
no
target
sensing
activity
normal
cells.
Combining
fluorescence-
surface-enhanced
Raman
scattering
(FL/SERS)
techniques,
this
exhibits
significantly
improved
selectivity
microRNA
This
study
provides
a
new
paradigm
develop
EDC-based
platform
shows
great
potential
in-depth
diagnosis
high
precision.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Herein,
a
supramolecular
DNA
nanodevice
was
formed
via
the
rolling
circle
amplification
(RCA)
and
hybridization
chain
reaction
(HCR)
cascade
on
tetrahedral
nanostructure
(TDN)
to
achieve
simultaneous
sensitive
detection
intracellular
imaging
of
dual-miRNAs
related
liver
cancer.
The
effectively
addressed
limitations
low
probe
loading
capacity
in
traditional
TDN
nanodevices
by
enriching
plenty
signal
probes
around
single
TDN,
significantly
enhancing
fluorescence
signal.
Impressively,
with
fulcrum
dense
structure
imparted
strong
rigidity,
ensuring
stability
decrease
aggregation
quenching
for
further
increasing
response.
Consequently,
biosensor
based
enabled
miRNA221
miRNA222,
achieved
accurate
situ
displaying
improved
capabilities
compared
TDN-based
nanodevices.
More
importantly,
precise
miRNA222
could
distinguish
hepatocellular
carcinoma
cells
different
degrees
metastasis
from
human
normal
cells,
providing
more
information
diagnosis
development
carcinoma.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 27, 2025
Fibroblast
phenotypic
transformation
plays
a
crucial
role
in
tumorigenesis.
Tumor
cell-derived
exosome-mediated
fibroblast
has
been
commonly
studied.
However,
there
is
still
no
direct
evidence
that
the
process
of
tumor
exosomes
induces
fibroblasts
into
cancer-associated
(CAFs).
Herein,
exosomal
miRNAs
were
used
as
target
to
track
invasion
cancer
normal
fibroblasts,
and
FAP
mRNA,
CAF
biomarker,
further
trace
effect
on
recipient
cells.
Two
DNA
tetrahedral
sensors
developed
based
hybrid
chain
reaction
signal
amplification
strategy
fluorescence
resonance
energy
transfer
mechanism.
Both
showed
high
sensitivity
good
selectivity,
with
detection
limits
4.94
6.61
pM
for
miR-1247-3p
respectively.
Moreover,
two
allowed
us
image
living
cell
postinvasion
tumor-derived
exosomes,
facilitating
visual
monitoring
phenotype
uncovering
new
perspectives
metastasis
development.
Analytical Methods,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
A
novel
inter-particle
DNA
walker
amplification
coupled
with
target
self-convert
for
sensitive
detection
of
miRNAs
by
liquid
chromatography
has
been
proposed.
