Advances in magnetic affinity-based isolation/detection of exosomes for robust diagnostics
Microchimica Acta,
Год журнала:
2025,
Номер
192(4)
Опубликована: Март 5, 2025
The
review
article
provides
a
short
introduction
to
exosomes
with
the
focus
use
as
disease
markers
itself
(i.e.
their
concentration
or
presence
of
some
specific
receptors)
source
biomarkers
such
proteins
and
metabolites.
In
detail,
we
are
discussing
various
methods
exosome
isolation
main
paper
is
on
affinity
capture
exosomes,
since
them
can
be
applied
sub-populations
produced
by
organs.
comprehensive
overview
magnetic
(bio)affinity
detection
exosomal
cargo
using
different
ligands
antibodies,
DNA
aptamers,
peptides,
glycan-based
recognition,
transferrin-based
approaches,
based
recognition
phospholipids
other
approaches
including
electrostatic
interactions.
in
detail
key
analytical
clinical
parameters
form
an
extensive
table
summarising
outcomes
published
last
two
years
(2023-2024).
Finally,
also
conclusions
sections
pros
cons
for
and/or
determination
content.
Язык: Английский
Electrochemical conjugation of surface proteins for sensitive detection of breast cancer extracellular vesicles
Journal of Electroanalytical Chemistry,
Год журнала:
2025,
Номер
unknown, С. 119088 - 119088
Опубликована: Март 1, 2025
Язык: Английский
Nanosized Membrane Fusion-Triggered Three-Dimensional DNA Walker for Subtype-Specific Analysis of Breast Cancer Extracellular Vesicles
Nano Letters,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 24, 2025
Extracellular
vesicles
(EVs)
are
highly
attractive
in
cancer
diagnosis,
owing
to
their
ability
reflect
the
molecular
characteristics
of
source
cells.
Herein,
we
engineer
a
DNA-equipped
liposome
nanoprobe
for
developing
nanosized
membrane
fusion-triggered
three-dimensional
(3D)
DNA
walker
analysis
breast
EVs.
Specifically,
nanoprobes
efficiently
fuse
with
target
EVs
by
recognizing
surface-expressed
epidermal
growth
factor
receptors,
thereby
creating
3D
tracks
walker.
Subsequently,
walking
probes
targeting
human
receptor-2
(HER-2)
were
recruited
onto
fused
manipulate
walker,
eventually
generating
considerable
electrochemical
signals.
Results
reveal
good
linearity
between
output
and
EV
concentration
also
prove
differentiate
HER-2-positive
patients
monitor
disease
progression.
Taken
together,
this
work
presents
an
effective
approach
early
subtype-specific
diagnosis
cancer.
Язык: Английский
Recent progress and challenges in ECL biosensors for the identification of small extracellular vesicle and clinical diagnosis
TrAC Trends in Analytical Chemistry,
Год журнала:
2025,
Номер
unknown, С. 118253 - 118253
Опубликована: Апрель 1, 2025
Язык: Английский
An Entropy-Driven Multipedal DNA Walker Microsensor for In Situ Electrochemical Detection of ATP
Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 19, 2024
Microelectrode-
and
nanoelectrode-based
electrochemistry
has
become
a
powerful
tool
for
the
in
situ
monitoring
of
various
biomolecules
vivo.
However,
two
challenges
limit
application
micro-
nanoelectrodes:
difficulty
highly
sensitive
detection
nonelectroactive
molecules
specific
target
complex
biological
environments.
Herein,
we
propose
an
electrochemical
microsensor
based
on
entropy-driven
multipedal
DNA
walker
selective
ATP.
An
ATP
aptamer
was
immobilized
microelectrode
surface
to
form
track
recognition.
Multiple
walking
"legs"
were
attached
quantum
dots
create
walker.
selectively
captured
by
aptamer,
triggering
autonomous
movement
interface,
which
brought
methylene
blue
(MB)
closer
realizing
cascade
signal
amplification.
Additionally,
release
induced
hypotonic
conditions
BV2
cells
successfully
monitored,
indicating
that
increased
cell
volume
enhances
release.
This
offers
novel
strategy
situ,
sensitive,
vivo,
is
crucial
investigating
neurochemical
processes
involved
neurological
diseases.
Язык: Английский