An Entropy-Driven Multipedal DNA Walker Microsensor for In Situ Electrochemical Detection of ATP DOI
Hong Jiang, Xiao Liu, Dong Zhao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Microelectrode- and nanoelectrode-based electrochemistry has become a powerful tool for the in situ monitoring of various biomolecules vivo. However, two challenges limit application micro- nanoelectrodes: difficulty highly sensitive detection nonelectroactive molecules specific target complex biological environments. Herein, we propose an electrochemical microsensor based on entropy-driven multipedal DNA walker selective ATP. An ATP aptamer was immobilized microelectrode surface to form track recognition. Multiple walking "legs" were attached quantum dots create walker. selectively captured by aptamer, triggering autonomous movement interface, which brought methylene blue (MB) closer realizing cascade signal amplification. Additionally, release induced hypotonic conditions BV2 cells successfully monitored, indicating that increased cell volume enhances release. This offers novel strategy situ, sensitive, vivo, is crucial investigating neurochemical processes involved neurological diseases.

Language: Английский

Advances in magnetic affinity-based isolation/detection of exosomes for robust diagnostics DOI Creative Commons

Veronika Solovicová,

Anna Ďatková,

Tomáš Bertók

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(4)

Published: March 5, 2025

The review article provides a short introduction to exosomes with the focus use as disease markers itself (i.e. their concentration or presence of some specific receptors) source biomarkers such proteins and metabolites. In detail, we are discussing various methods exosome isolation main paper is on affinity capture exosomes, since them can be applied sub-populations produced by organs. comprehensive overview magnetic (bio)affinity detection exosomal cargo using different ligands antibodies, DNA aptamers, peptides, glycan-based recognition, transferrin-based approaches, based recognition phospholipids other approaches including electrostatic interactions. in detail key analytical clinical parameters form an extensive table summarising outcomes published last two years (2023-2024). Finally, also conclusions sections pros cons for and/or determination content.

Language: Английский

Citations

1
Electrochemical conjugation of surface proteins for sensitive detection of breast cancer extracellular vesicles DOI
Junda Li, Jianhua Guan, Yuan Lin

et al.

Journal of Electroanalytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 119088 - 119088

Published: March 1, 2025

Language: Английский

Citations

0
Nanosized Membrane Fusion-Triggered Three-Dimensional DNA Walker for Subtype-Specific Analysis of Breast Cancer Extracellular Vesicles DOI

Zhikai Fang,

A‐Yong Cao, Zichen Huang

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Extracellular vesicles (EVs) are highly attractive in cancer diagnosis, owing to their ability reflect the molecular characteristics of source cells. Herein, we engineer a DNA-equipped liposome nanoprobe for developing nanosized membrane fusion-triggered three-dimensional (3D) DNA walker analysis breast EVs. Specifically, nanoprobes efficiently fuse with target EVs by recognizing surface-expressed epidermal growth factor receptors, thereby creating 3D tracks walker. Subsequently, walking probes targeting human receptor-2 (HER-2) were recruited onto fused manipulate walker, eventually generating considerable electrochemical signals. Results reveal good linearity between output and EV concentration also prove differentiate HER-2-positive patients monitor disease progression. Taken together, this work presents an effective approach early subtype-specific diagnosis cancer.

Language: Английский

Citations

0
Recent progress and challenges in ECL biosensors for the identification of small extracellular vesicle and clinical diagnosis DOI
Shijie Li,

Peilin Wang,

Qiang Ma

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118253 - 118253

Published: April 1, 2025

Language: Английский

Citations

0
An Entropy-Driven Multipedal DNA Walker Microsensor for In Situ Electrochemical Detection of ATP DOI
Hong Jiang, Xiao Liu, Dong Zhao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Microelectrode- and nanoelectrode-based electrochemistry has become a powerful tool for the in situ monitoring of various biomolecules vivo. However, two challenges limit application micro- nanoelectrodes: difficulty highly sensitive detection nonelectroactive molecules specific target complex biological environments. Herein, we propose an electrochemical microsensor based on entropy-driven multipedal DNA walker selective ATP. An ATP aptamer was immobilized microelectrode surface to form track recognition. Multiple walking "legs" were attached quantum dots create walker. selectively captured by aptamer, triggering autonomous movement interface, which brought methylene blue (MB) closer realizing cascade signal amplification. Additionally, release induced hypotonic conditions BV2 cells successfully monitored, indicating that increased cell volume enhances release. This offers novel strategy situ, sensitive, vivo, is crucial investigating neurochemical processes involved neurological diseases.

Language: Английский

Citations

1