Biomolecules based hydrogels and their potential biomedical applications: A comprehensive review

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 253, С. 127362 - 127362

Опубликована: Окт. 10, 2023

Язык: Английский

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Redox-enabled electronic interrogation and feedback control of hierarchical and networked biological systems

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Дек. 21, 2023

Abstract Microelectronic devices can directly communicate with biology, as electronic information be transmitted via redox reactions within biological systems. By engineering biology’s native networks, we enable interrogation and control of systems at several hierarchical levels: proteins, cells, cell consortia. First, electro-biofabrication facilitates on-device component assembly. Then, electrode-actuated data transmission redox-linked synthetic biology allows programming enzyme activity closed-loop electrogenetic cellular function. Specifically, horseradish peroxidase is assembled onto interdigitated electrodes where electrode-generated hydrogen peroxide controls its activity. E. coli ’s stress response regulon, oxyRS , rewired to algorithm-based feedback gene expression, including an eCRISPR module that switches cell-cell quorum sensing communication from one autoinducer another—creating electronically controlled ‘bilingual’ cell. these disparate redox-guided are wirelessly connected, enabling real-time user-based control. We suggest methodologies will help us better understand develop sophisticated for biology.

Язык: Английский

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Electrodeposited Carboxymethyl Cellulose‐Based Green Preparation of Nanosilver With Adjustable Sizes and Nanocomposite Films Through Controlling Electrodeposition Conditions

Journal of Polymer Science, Год журнала: 2025, Номер unknown

Опубликована: Март 7, 2025

ABSTRACT Electrodeposition of polysaccharides offers an attractive strategy for fabricating nanoparticles and their nanocomposite films on electrodes to develop bioelectronic devices functional materials. Herein, a controllable approach the green preparation nanosilver (nAg) with variable sizes is developed based coordinated electrodeposition carboxymethyl cellulose (CMC). In addition serving as electrodeposited polysaccharide, CMC can function reducing agent stabilizer preparing nAg. After electrodeposition, uniform smooth (nAg/CMC films) have been deposited anodic electrode. Spectral analysis transmission electron microscopy confirm presence nAg in films. Especially, adjustable particle (ranging from 2.03 13.62 nm) be achieved through straightforward convenient control conditions (voltage, temperature). Moreover, endowed unique fluorescent properties since silver nanoclusters (Ag NCs) relatively small size controllably prepared. The nAg/CMC film‐modified show potential electrochemical detection that film containing small‐sized Ag NCs exhibits good sensitivity. Thus, this study provides novel, facile, controllable, eco‐friendly promisingly applied prepare nanocomposites specific special properties.

Язык: Английский

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Chitin/Chitosan Biocomposite Foams with Chitins from Different Organisms for Sound Absorption

ACS Sustainable Chemistry & Engineering, Год журнала: 2024, Номер 12(32), С. 11879 - 11890

Опубликована: Июль 9, 2024

Foams are widely used for applications in construction, energy absorption, and building insulation. We developed sustainable chitin/chitosan-based foams derived from snow crab Aspergillus niger (α-chitin) squid (β-chitin), which were obtained via a "shake bake" process. The foam structure, mechanical, thermophysical, sound flammability properties studied. Stable chitin, whereas A.niger-based inhomogeneous. the former biomass sources displayed densities of 0.07–0.30 g/cm3 bulk porosities 78–94% with only minimal number closed pores. According to mercury porosimetry (MP) X-ray computed microtomography (μXRCT), pore sizes ranged 3 μm 1.5 mm, majority pores being larger than 400 μm. In mechanical compression tests, β-chitin-based showed higher specific compressive strength modulus (up σ0.1* = 9.00 MPa/E* 107.37 MPa) compared α-chitin-based series. Dynamic vapor sorption (DVS) measurements revealed that β-chitin (from squid) series overall took up more water (≤40 wt %) α-chitin crab) (≤33 %). Flammability tests suitable fire protection class E, superior common polyurethane (PU) foams, absorption promising results little influenced by humidity.

Язык: Английский

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Design of a porous lignocellulose-based hydrogel-Pd nanoparticles reactor system and its in-situ simultaneous adsorption-catalysis process concept for pollutant remedy

Separation and Purification Technology, Год журнала: 2023, Номер 332, С. 125751 - 125751

Опубликована: Ноя. 19, 2023

Язык: Английский

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Electrobiofabrication of antibody sensor interfaces within a 3D printed device yield rapid and robust electrochemical measurements of titer and glycan structure

Biotechnology and Bioengineering, Год журнала: 2024, Номер 121(12), С. 3754 - 3767

Опубликована: Сен. 16, 2024

We report the integration of 3D printing, electrobiofabrication, and protein engineering to create a device that enables near real-time analysis monoclonal antibody (mAb) titer quality. printing was used macroscale architecture can control fluidic contact sample with multiple electrodes for replicate measurements. An "chip" configured as "snap-in" module connecting printed housing containing electronic communication systems. Electrobiofabrication functionalize each electrode by assembly hydrogel interface biomolecular recognition capture proteins. Specifically, an electrochemical thiol oxidation is assemble thiolated polyethylene glycol hydrogel, in turn covalently coupled either cysteine-tagged G binds antibody's Fc region or lectin glycans target mAb analytes. first show design, assembly, testing hardware device. Then, we transition step-by-step sensing methodology (e.g., mix, incubate, wash, measure) into current method where functionalization, capture, assessment are performed situ parallel channels. Both glycan analyses were found be linear concentration (to 0.2 mg/L). further interfaces could reused remarkably similar results. Because use simple, rapid, robust, suggest this will widely applicable, including other process development manufacturing environments.

Язык: Английский

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Redox-enabled Electronic Interrogation and Feedback Control of Hierarchical and Networked Biological Systems

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 22, 2023

Abstract We enable microelectronic devices to interrogate biology’s molecular communication, perform computations, and in real time control biological systems, including at several hierarchical levels: proteins, cells, cell consortia. A key driver is establishing electronic access from native redox networks. First, redox-mediated electro-biofabrication facilitates facile assembly of components onto then electrode-actuated allows digital programming enzyme activity, further, electrogenetics closed-loop cellular function. Specifically, we show algorithm-based feedback genetic circuits (via eCRISPR) consortia behavior, all enabled by data transfer. further demonstrate switching cell-cell quorum sensing communication one autoinducer network another, creating an electronically controlled “bilingual” cell. suggest these methodologies will not only help us better understand but design those currently unimagined.

Язык: Английский

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Electronic inputs to cue the emergence of hydrogel structure and to confer function

Materials Letters, Год журнала: 2023, Номер 355, С. 135497 - 135497

Опубликована: Окт. 30, 2023

Язык: Английский

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Mechanically Adaptive Metal-Coordinated Electrogel Membranes

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(36), С. 48280 - 48292

Опубликована: Авг. 26, 2024

Achieving specific mechanical properties of hydrogels, especially when used as thin films, can be crucial in diverse applications, including tissue engineering and bioelectronics. Here, a novel electrochemical approach for fabricating uniform robust hydrogel films based on carboxymethyl cellulose cross-linked by Fe3+ ions (Fe-CMC), exhibiting tunable, dynamic is introduced. High modulation the film achieved applying multiple pulses oxidative voltage during deposition. Our study shows also remarkable effect ionic strength electrodeposited films. We found that switching from salt solution to water enhanced stiffness thereby regulating permeability These results are supported molecular dynamics (MD) simulations, showing an increase induces weakening Fe-CMC interactions, ultimately affecting network strength. Finally, robustness these enables their delamination electrode without any damage, expanding potential applications freestanding smart membranes. By providing fundamental insights into metal-coordinated bonds response at macroscopic scale, we have demonstrated versatility gelation fabrication with tunable properties, which could serve platforms variety biomedical applications.

Язык: Английский

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Target-Induced On-Protein Clustering of Metal Peptide Enables Low Overpotential Water Splitting for Early Detection of Non-Small-Cell Lung Cancer

ACS Sensors, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 20, 2024

This study presents a novel method for the early detection of non-small-cell lung cancer (NSCLC) by employing target-induced on-protein clustering metal-peptide complexes to facilitate low overpotential water splitting. The approach utilizes designed peptide molecular probe composed an EGFR-targeting motif and copper-chelating tetrapeptide. Upon interaction with epidermal growth factor receptor (EGFR) divalent copper ions, forms stable complex that undergoes clustering. significantly amplifies electrochemical signal through enhanced dityrosine cross-linking subsequent splitting, achieving detection. was validated using clinical tissue samples demonstrated improved sensitivity specificity compared traditional methods. technique holds promise earlier more accurate diagnosis NSCLC, leveraging unique properties interactions amplification.

Язык: Английский

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