Engineered Protein Hydrogels as Biomimetic Cellular Scaffolds
Advanced Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 5, 2024
Abstract
The
biochemical
and
biophysical
properties
of
the
extracellular
matrix
(ECM)
play
a
pivotal
role
in
regulating
cellular
behaviors
such
as
proliferation,
migration,
differentiation.
Engineered
protein‐based
hydrogels,
with
highly
tunable
multifunctional
properties,
have
potential
to
replicate
key
features
native
ECM.
Formed
by
self‐assembly
or
crosslinking,
engineered
hydrogels
can
induce
range
cell
through
bioactive
functional
domains
incorporated
into
polymer
backbone.
Using
recombinant
techniques,
amino
acid
sequence
protein
backbone
be
designed
precise
control
over
chain‐length,
folded
structure,
cell‐interaction
sites.
In
this
review,
modular
design
from
both
molecular‐
network‐level
perspective
are
discussed,
summarize
recent
progress
case
studies
highlight
diverse
strategies
used
construct
biomimetic
scaffolds.
This
review
focuses
on
sequences
that
form
structural
blocks,
stimuli‐responsive
blocks
for
scaffold
properties.
Both
physical
chemical
methods
stabilize
dynamic
networks
defined
structure
bioactivity
culture
applications
discussed.
Finally,
discussion
future
directions
scaffolds
is
concluded.
Язык: Английский
Di-Tyrosine Cross-Linking of Elastin-Like Polypeptides through Ruthenium Photoreaction To Form Scaffolds: Fine Tuning Mechanical Properties and Improving Cytocompatibility
Iyeswaria K Amma,
Rohan S.J. Ingrole,
G. Prabhu
и другие.
Biomacromolecules,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 19, 2025
Ensuring
that
the
mechanical
properties
of
tissue
engineering
scaffolds
align
with
those
target
tissues
is
crucial
for
their
successful
integration
and
functional
performance.
Tyrosine–tyrosine
cross-links
are
found
in
nature
numerous
proteins
including
resilin
exhibit
enhanced
toughness
energy
storage
capacity.
Herein,
we
investigated
potential
tuning
made
from
elastin-like
polypeptides
(ELPs)
containing
tyrosine
residues.
Ruthenium-based
photoreaction
was
used
to
form
cross-links.
To
enhance
cytocompatibility
ELP
scaffold,
a
continuous
mode
washing
developed
remove
residual
ruthenium
scaffolds.
The
significantly
superior
removing
did
so
shorter
time
as
compared
batch
conventional
semibatch
(also
called
dialysis
washing).
range
moduli
fabricated
spanned
tens
Pa
hundreds
kPa.
Human
fibroblast
cells
were
grow
proliferate.
Overall,
this
work
offers
rationale
further
developing
cross-linked
ELPs
broad
applications.
Язык: Английский
Protease-Driven Phase Separation of Elastin-Like Polypeptides
Biomacromolecules,
Год журнала:
2024,
Номер
25(8), С. 4898 - 4904
Опубликована: Июль 9, 2024
Elastin-like
polypeptides
(ELPs)
are
a
promising
material
platform
for
engineering
stimuli-responsive
biomaterials,
as
ELPs
undergo
phase
separation
above
tunable
transition
temperature.
with
behavior
that
is
isothermally
regulated
by
biological
stimuli
remain
attractive
applications
in
systems.
Herein,
we
report
protease-driven
of
ELPs.
Protease-responsive
"cleavable"
comprise
hydrophobic
ELP
block
connected
to
hydrophilic
protease
cleavage
site
linker.
The
acts
solubility
tag
the
block,
creating
temperature
window
which
cleavable
reactant
soluble
and
proteolytically
generated
insoluble.
Within
this
window,
isothermal,
occurs
when
critical
concentration
product
accumulates.
Furthermore,
generalizable
four
compatible
protease-cleavable
pairings.
This
work
presents
exciting
opportunities
regulate
systems
using
proteases.
Язык: Английский
Programmability and biomedical utility of intrinsically-disordered protein polymers
Advanced Drug Delivery Reviews,
Год журнала:
2024,
Номер
212, С. 115418 - 115418
Опубликована: Июль 31, 2024
Язык: Английский
Organoid bioprinting: from cells to functional tissues
Nature Reviews Bioengineering,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 16, 2024
Язык: Английский
PhoCoil: An Injectable and Photodegradable Single-component Recombinant Protein Hydrogel for Localized Therapeutic Cell Delivery
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 10, 2024
Abstract
Hydrogel
biomaterials
offer
great
promise
for
3D
cell
culture
and
therapeutic
delivery.
Despite
many
successes,
challenges
persist
in
that
gels
formed
from
natural
proteins
are
only
marginally
tunable
while
those
derived
synthetic
polymers
lack
intrinsic
bioinstructivity.
Towards
the
creation
of
with
both
excellent
biocompatibility
customizability,
recombinant
protein-based
hydrogels
have
emerged
as
molecularly
defined
user-programmable
platforms
mimic
proteinaceous
nature
extracellular
matrix.
Here,
we
introduce
PhoCoil,
a
dynamically
hydrogel
single
protein
component
unique
multi-stimuli
responsiveness.
Physical
crosslinking
through
coiled-coil
interactions
promotes
rapid
shear-thinning
self-healing
behavior,
rendering
gel
injectable,
an
included
photodegradable
motif
affords
on-demand
network
dissolution
via
visible
light.
PhoCoil
photodegradation
can
be
spatiotemporally
lithographically
controlled
dose-dependent
manner,
complex
tissue,
without
harm
to
encapsulated
cells.
We
anticipate
will
enable
new
applications
tissue
engineering
regenerative
medicine.
Язык: Английский
Contribution of the ELRs to the development of advanced in vitro models
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Апрель 8, 2024
Developing
in
vitro
models
that
accurately
mimic
the
microenvironment
of
biological
structures
or
processes
holds
substantial
promise
for
gaining
insights
into
specific
functions.
In
field
tissue
engineering
and
regenerative
medicine,
able
to
capture
precise
structural,
topographical,
functional
complexity
living
tissues,
prove
be
valuable
tools
comprehending
disease
mechanisms,
assessing
drug
responses,
serving
as
alternatives
complements
animal
testing.
The
choice
right
biomaterial
fabrication
technique
development
these
plays
an
important
role
their
functionality.
this
sense,
elastin-like
recombinamers
(ELRs)
have
emerged
tool
overcoming
challenges
encountered
natural
synthetic
materials
due
intrinsic
properties,
such
phase
transition
behavior,
tunable
viscoelasticity,
easy
processability.
review
article,
we
will
delve
use
ELRs
molecular
intrinsically
disordered
proteins
(IDPs),
well
3D
medicine.
processability
rational
design
has
allowed
spheroids
organoids,
bioinks
bioprinting.
Thus,
incorporating
toolkit
biomaterials
used
models,
represents
a
transformative
step
forward
improving
accuracy,
efficiency,
functionality
opening
up
wide
range
possibilities
combination
with
advanced
biofabrication
techniques
remains
explored.
Язык: Английский