Biomacromolecules,
Год журнала:
2024,
Номер
26(1), С. 670 - 678
Опубликована: Дек. 19, 2024
Hydrogel
three-dimensional
(3D)
printing
has
emerged
as
a
highly
valuable
fabrication
tool
for
applications
ranging
from
electronics
and
biomedicine.
While
conventional
hydrogels
such
gelatin,
alginate,
hyaluronic
acid
satisfy
biocompatibility
requirements,
they
distinctly
lack
reproducibility
in
terms
of
mechanical
properties
3D
printability.
Aiming
to
offer
high-performance
alternative,
here
we
present
range
amphiphilic
star-shaped
diblock
copolypeptides
l-glutamate
l-leucine
residues
with
different
topologies.
Hydrophobic
side
chains
the
polymer
block
drive
conformational
self-assembly
water,
spontaneously
forming
tunable
properties,
through
variation
star
topology.
Their
amenable
shear-thinning
self-recovery
render
them
suitable
hydrogel
inks
direct
ink
writing.
Well-defined
3D-printed
structures
can
be
readily
generated
rapidly
photo-cross-linked
using
visible
light
(405
nm)
after
methacrylamide
functionalization,
while
demonstrate
good
top-seeded
encapsulated
MC3T3
cells.
Environmental Science & Technology,
Год журнала:
2024,
Номер
58(23), С. 9954 - 9966
Опубликована: Май 28, 2024
Per-
and
polyfluoroalkyl
substances
(PFAS)
strongly
bind
to
proteins
lipids
in
blood,
which
govern
their
accumulation
distribution
organisms.
Understanding
the
plasma
binding
mechanism
species
differences
will
facilitate
quantitative
Gels,
Год журнала:
2024,
Номер
10(12), С. 780 - 780
Опубликована: Ноя. 28, 2024
The
abundance
of
hyaluronic
acid
(HA)
in
human
tissues
attracts
its
thorough
research
tissue
regenerating
scaffolds
and
3D
bioprintable
hydrogel
preparation.
Though
methacrylation
HA
can
lead
to
photo-crosslinkable
hydrogels,
the
catalyst
has
toxicity
concerns,
is
not
suitable
for
creating
stable
complex
structures
using
extrusion
bioprinting.
In
this
study,
a
dual
crosslinking
on
methacrylated
introduced,
cysteamine-grafted
varying
concentrations
2-hydroxy
ethyl
acrylate.
resultant
printing
(or
bioprinting),
mechanically
robust,
self-standing,
phosphate-buffered
saline
at
37
°C
more
than
42
days,
high
water
absorption
capacity
with
low
swelling
ratio
(1.5),
exhibits
self-healing
adhesive
properties.
Complex
like
ears
pyramid
shapes
2
cm
height
are
printed
optimized
composition.
All
synthesized
hydrogels
have
shown
nontoxicity
cell-supportiveness.
Loading
cells,
tetracycline,
bovine
serum
albumin
into
led
better
bioink
properties
such
as
cell
attachment,
growth,
proliferation
osteoblast
cells.
test
results
suggest
that
biocompatible
potential
bioprinting
self-standing
form
engineering
regenerative
medicine.
Environmental Science & Technology,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 24, 2024
Internal
concentrations
(ICs)
are
crucial
for
linking
exposure
to
effects
in
the
development
of
New
Approach
Methodologies.
ICs
chemicals
aquatic
organisms
primarily
driven
by
hydrophobicity
and
modulated
biotransformation
efflux.
Comparing
predicted
baseline
observed
toxicity
enables
estimation
effect
specificity,
but
biological
processes
can
lead
overestimating
bias
specificity
assessment.
To
evaluate
prediction
a
mass
balance
model
(MBM)
impact
on
ICs,
experimental
63
zebrafish
embryos
were
compared
predictions
with
physicochemical
properties
as
input
parameters.
Experimental
79%
(50
63)
deviated
less
than
10-fold
from
predictions,
remaining
13
up
factor
90.
Using
changed
classification
19
chemicals,
5
90
times
lower
predicted,
showing
classification.
Uptake
kinetics
pirinixic
acid,
genistein,
dexamethasone,
ethoprophos,
atorvastatin,
niflumic
acid
studied
over
96
h
period,
transformation
products
(TPs)
elucidated
using
suspect-
nontarget
screening
UPLC-HRMS.
35
TPs
(5
8
per
compound)
tentatively
identified
semiquantified
based
peak
areas,
suggesting
that
may
partly
account
overpredictions
ICs.
Antioxidants,
Год журнала:
2024,
Номер
13(12), С. 1431 - 1431
Опубликована: Ноя. 21, 2024
Acrylamide
(ACR)
is
a
low-molecular
weight,
non-aromatic
reagent,
widely
used
in
industry,
such
as
the
manufacture
of
paper,
textiles,
plastics,
cosmetics,
and
dyes.
ACR
formed
during
cooking
starchy
food
its
toxicity
results
mainly
by
conferring
oxidative
stress
elevating
reactive
oxygen
species
(ROS).
To
identify
potential
antidotes
for
toxicity,
we
evaluated
efficacy
several
thiol-based
molecules
known
ROS-scavenging,
disulfide-reducing
properties,
inhibition
stress-induced
activation
mitogen-activated
protein
kinases
(MAPKs):
extracellular-signal-regulated-kinases
(ERK1/2),
p38-mitogen-activated-protein-kinases
(p38MAPK),
c-Jun-N-terminal-kinases
(JNKs).
We
established
reproducible
assay
testing
N-acetylcysteine
(NAC),
AD4/NACA,
N-and
C-blocked
tri-
tetra-thioredoxin-mimetic
(TXM)
peptides,
PC12
cells.
Our
demonstrate
that
these
compounds
exhibited
high
suppressing
ACR-induced
MAPK
activation,
either
prior
to
or
subsequent
exposure.
The
single
cysteine
(Cys)
residue,
NAC
AD4/NACA
(NAC-amide),
2
Cys
peptides
TXM-CB30,
AcDCys-Gly-DCysNH2,
TXM-CB20,
AcCys-Gly-CysNH2,
SuperDopa
(SD,
Ac-CysL-Levodopa-CysNH2,
TXM-CB13,
AcCys-Met-Lys-CysNH2,
3-Cys
peptide,
TXM-CB16,
AcCys-γGlu-Cys-CysNH2
was
dose-dependent
potency
displayed
direct
correlation
with
number
residues.
Cellular
proteolysis
SD,
which
consists
levodopa
flanked
two
Cys,
may
suppress
manifestation
Parkinson’s
disease
(PD)-like
symptoms
mediated
chronic
exposure
not
only
through
lowering
but
also
replenishing
cellular
levels
dopamine.
Overall,
could
advance
clinical
application
TXM
treatments
acute
and/or
show
promise
preventing
ACR-triggered
PD-like
neurotoxic
symptoms.
Biomacromolecules,
Год журнала:
2024,
Номер
26(1), С. 670 - 678
Опубликована: Дек. 19, 2024
Hydrogel
three-dimensional
(3D)
printing
has
emerged
as
a
highly
valuable
fabrication
tool
for
applications
ranging
from
electronics
and
biomedicine.
While
conventional
hydrogels
such
gelatin,
alginate,
hyaluronic
acid
satisfy
biocompatibility
requirements,
they
distinctly
lack
reproducibility
in
terms
of
mechanical
properties
3D
printability.
Aiming
to
offer
high-performance
alternative,
here
we
present
range
amphiphilic
star-shaped
diblock
copolypeptides
l-glutamate
l-leucine
residues
with
different
topologies.
Hydrophobic
side
chains
the
polymer
block
drive
conformational
self-assembly
water,
spontaneously
forming
tunable
properties,
through
variation
star
topology.
Their
amenable
shear-thinning
self-recovery
render
them
suitable
hydrogel
inks
direct
ink
writing.
Well-defined
3D-printed
structures
can
be
readily
generated
rapidly
photo-cross-linked
using
visible
light
(405
nm)
after
methacrylamide
functionalization,
while
demonstrate
good
top-seeded
encapsulated
MC3T3
cells.
