Tuning Star Polymer Architecture to Tailor Secondary Structures and Mechanical Properties of Diblock Polypeptide Hydrogels for Direct Ink Writing DOI Creative Commons

Muireann Cosgrave,

Kulwinder Kaur, Christopher D. Simpson

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 26(1), P. 670 - 678

Published: Dec. 19, 2024

Hydrogel three-dimensional (3D) printing has emerged as a highly valuable fabrication tool for applications ranging from electronics and biomedicine. While conventional hydrogels such gelatin, alginate, hyaluronic acid satisfy biocompatibility requirements, they distinctly lack reproducibility in terms of mechanical properties 3D printability. Aiming to offer high-performance alternative, here we present range amphiphilic star-shaped diblock copolypeptides l-glutamate l-leucine residues with different topologies. Hydrophobic side chains the polymer block drive conformational self-assembly water, spontaneously forming tunable properties, through variation star topology. Their amenable shear-thinning self-recovery render them suitable hydrogel inks direct ink writing. Well-defined 3D-printed structures can be readily generated rapidly photo-cross-linked using visible light (405 nm) after methacrylamide functionalization, while demonstrate good top-seeded encapsulated MC3T3 cells.

Language: Английский

Impact of Biotransformation on Internal Concentrations and Specificity Classification of Organic Chemicals in the Zebrafish Embryo (Danio rerio) DOI Creative Commons
Nico Grasse, Riccardo Massei, Bettina Seiwert

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Internal concentrations (ICs) are crucial for linking exposure to effects in the development of New Approach Methodologies. ICs chemicals aquatic organisms primarily driven by hydrophobicity and modulated biotransformation efflux. Comparing predicted baseline observed toxicity enables estimation effect specificity, but biological processes can lead overestimating bias specificity assessment. To evaluate prediction a mass balance model (MBM) impact on ICs, experimental 63 zebrafish embryos were compared predictions with physicochemical properties as input parameters. Experimental 79% (50 63) deviated less than 10-fold from predictions, remaining 13 up factor 90. Using changed classification 19 chemicals, 5 90 times lower predicted, showing classification. Uptake kinetics pirinixic acid, genistein, dexamethasone, ethoprophos, atorvastatin, niflumic acid studied over 96 h period, transformation products (TPs) elucidated using suspect- nontarget screening UPLC-HRMS. 35 TPs (5 8 per compound) tentatively identified semiquantified based peak areas, suggesting that may partly account overpredictions ICs.

Language: Английский

Citations

0
Thiol-Based Redox Molecules: Potential Antidotes for Acrylamide Toxicity DOI Creative Commons

Valeria Martin,

Michael Trus,

Daphné Atlas

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1431 - 1431

Published: Nov. 21, 2024

Acrylamide (ACR) is a low-molecular weight, non-aromatic reagent, widely used in industry, such as the manufacture of paper, textiles, plastics, cosmetics, and dyes. ACR formed during cooking starchy food its toxicity results mainly by conferring oxidative stress elevating reactive oxygen species (ROS). To identify potential antidotes for toxicity, we evaluated efficacy several thiol-based molecules known ROS-scavenging, disulfide-reducing properties, inhibition stress-induced activation mitogen-activated protein kinases (MAPKs): extracellular-signal-regulated-kinases (ERK1/2), p38-mitogen-activated-protein-kinases (p38MAPK), c-Jun-N-terminal-kinases (JNKs). We established reproducible assay testing N-acetylcysteine (NAC), AD4/NACA, N-and C-blocked tri- tetra-thioredoxin-mimetic (TXM) peptides, PC12 cells. Our demonstrate that these compounds exhibited high suppressing ACR-induced MAPK activation, either prior to or subsequent exposure. The single cysteine (Cys) residue, NAC AD4/NACA (NAC-amide), 2 Cys peptides TXM-CB30, AcDCys-Gly-DCysNH2, TXM-CB20, AcCys-Gly-CysNH2, SuperDopa (SD, Ac-CysL-Levodopa-CysNH2, TXM-CB13, AcCys-Met-Lys-CysNH2, 3-Cys peptide, TXM-CB16, AcCys-γGlu-Cys-CysNH2 was dose-dependent potency displayed direct correlation with number residues. Cellular proteolysis SD, which consists levodopa flanked two Cys, may suppress manifestation Parkinson’s disease (PD)-like symptoms mediated chronic exposure not only through lowering but also replenishing cellular levels dopamine. Overall, could advance clinical application TXM treatments acute and/or show promise preventing ACR-triggered PD-like neurotoxic symptoms.

Language: Английский

Citations

0
Tuning Star Polymer Architecture to Tailor Secondary Structures and Mechanical Properties of Diblock Polypeptide Hydrogels for Direct Ink Writing DOI Creative Commons

Muireann Cosgrave,

Kulwinder Kaur, Christopher D. Simpson

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 26(1), P. 670 - 678

Published: Dec. 19, 2024

Hydrogel three-dimensional (3D) printing has emerged as a highly valuable fabrication tool for applications ranging from electronics and biomedicine. While conventional hydrogels such gelatin, alginate, hyaluronic acid satisfy biocompatibility requirements, they distinctly lack reproducibility in terms of mechanical properties 3D printability. Aiming to offer high-performance alternative, here we present range amphiphilic star-shaped diblock copolypeptides l-glutamate l-leucine residues with different topologies. Hydrophobic side chains the polymer block drive conformational self-assembly water, spontaneously forming tunable properties, through variation star topology. Their amenable shear-thinning self-recovery render them suitable hydrogel inks direct ink writing. Well-defined 3D-printed structures can be readily generated rapidly photo-cross-linked using visible light (405 nm) after methacrylamide functionalization, while demonstrate good top-seeded encapsulated MC3T3 cells.

Language: Английский

Citations

0