Tailoring the photoluminescence of atomically precise nanoclusters

Chemical Society Reviews, Год журнала: 2019, Номер 48(8), С. 2422 - 2457

Опубликована: Янв. 1, 2019

Fluorescent nanoclusters are an emerging class of modular nanomaterials. This review highlights recent developments in tailoring the photo-luminescence nanoclusters.

Язык: Английский

---

Photochromism into nanosystems: towards lighting up the future nanoworld

Chemical Society Reviews, Год журнала: 2017, Номер 47(3), С. 1044 - 1097

Опубликована: Дек. 18, 2017

This review provides a state-of-the-art account of photochromism into diverse functional nanosystems such as nanomachines, nanoparticles, nanoelectronics, nanoassemblies and biological nanosystems.

Язык: Английский

---

Multireference Approaches for Excited States of Molecules

Chemical Reviews, Год журнала: 2018, Номер 118(15), С. 7293 - 7361

Опубликована: Июль 24, 2018

Understanding the properties of electronically excited states is a challenging task that becomes increasingly important for numerous applications in chemistry, molecular physics, biology, and materials science. A substantial impact exerted by fascinating progress time-resolved spectroscopy, which leads to strongly growing demand theoretical methods describe characteristic features accurately. Whereas electronic ground state problems stable molecules quantum chemical methodology now so well developed informed nonexperts can use it efficiently, situation entirely different concerning investigation states. This review devoted specific class approaches, usually denoted as multireference (MR) methods, generality needed solving many spectroscopic or photodynamical problems. However, understanding proper application these MR often found be difficult due their complexity computational cost. The purpose this provide an overview most facts about approaches available present means collection examples useful information, guide reader performing own applications.

Язык: Английский

---

Theory and Simulation of the Ultrafast Double-Bond Isomerization of Biological Chromophores

Chemical Reviews, Год журнала: 2017, Номер 117(22), С. 13502 - 13565

Опубликована: Окт. 30, 2017

Ultrafast processes in light-absorbing proteins have been implicated the primary step light-to-energy conversion and initialization of photoresponsive biological functions. Theory computations played an instrumental role understanding molecular mechanism such processes, as they provide a molecular-level insight structural electronic changes at ultrafast time scales that often are very difficult or impossible to obtain from experiments alone. Among theoretical strategies, application hybrid quantum mechanics (QM/MM) models is important approach has reached evident degree maturity, resulting several contributions field. This review presents overview state-of-the-art computational studies on subnanosecond events rhodopsins, photoactive yellow proteins, phytochromes, some other where photoinduced double-bond isomerization occurs. The also discusses current limitations need be solved future developments.

Язык: Английский

---

Photoluminescent Nanoparticles for Chemical and Biological Analysis and Imaging

Chemical Reviews, Год журнала: 2021, Номер 121(15), С. 9243 - 9358

Опубликована: Июль 20, 2021

Research related to the development and application of luminescent nanoparticles (LNPs) for chemical biological analysis imaging is flourishing. Novel materials new applications continue be reported after two decades research. This review provides a comprehensive heuristic overview this field. It targeted both newcomers experts who are interested in critical assessment LNP materials, their properties, strengths weaknesses, prospective applications. Numerous cataloged by fundamental descriptions identities physical morphology, quantitative photoluminescence (PL) PL mechanisms, surface chemistry. These include various semiconductor quantum dots, carbon nanotubes, graphene derivatives, nanodiamonds, metal nanoclusters, lanthanide-doped upconversion downshifting nanoparticles, triplet–triplet annihilation persistent-luminescence conjugated polymer semiconducting multi-nanoparticle assemblies, doped labeled including but not limited those based on polymers silica. As an exercise these ranked several application-related functional criteria. Additional sections highlight recent examples advances analysis, point-of-care diagnostics, cellular, tissue, vivo theranostics. drawn from literature organized material particular properties that leveraged advantage. Finally, perspective what comes next field offered.

Язык: Английский

---

An introduction to optical super-resolution microscopy for the adventurous biologist

Methods and Applications in Fluorescence, Год журнала: 2018, Номер 6(2), С. 022003 - 022003

Опубликована: Фев. 9, 2018

Ever since the inception of light microscopy, laws physics have seemingly thwarted every attempt to visualize processes life at its most fundamental, sub-cellular, level. The diffraction limit has restricted our view length scales well above 250 nm and in doing so, severely compromised ability gain true insights into many biological systems. Fortunately, continuous advancements optics, electronics mathematics provided means once again make work advantage. Even though some fundamental concepts enabling super-resolution microscopy been known for quite time, practically feasible implementations long remained elusive. It should therefore not come as a surprise that 2014 Nobel Prize Chemistry was awarded scientists who, each their own way, contributed transforming from technological tour de force staple biologist's toolkit. By overcoming barrier, could be established an indispensable tool age where importance understanding molecular level cannot overstated. This review strives provide aspiring science researcher with introduction optical starting governing compound fluorescent confocal current state-of-the-art techniques applications.

Язык: Английский

---

Split Green Fluorescent Proteins: Scope, Limitations, and Outlook

Annual Review of Biophysics, Год журнала: 2019, Номер 48(1), С. 19 - 44

Опубликована: Фев. 20, 2019

Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, a functional protein. For green fluorescent (GFPs), fragment reassembly leads to readout, which has been widely used investigate protein–protein interactions. We review the scope and limitations of this approach as well other diverse applications GFPs versatile sensors, molecular glues, optogenetic tools, platforms for photophysical studies.

Язык: Английский

---

Spectroscopy in Complex Environments from QM–MM Simulations

Chemical Reviews, Год журнала: 2018, Номер 118(7), С. 4071 - 4113

Опубликована: Март 21, 2018

The applications of multiscale quantum–classical (QM–MM) approaches have shown an extraordinary expansion and diversification in the last couple decades. A great proportion these efforts been devoted to interpreting reproducing spectroscopic experiments a variety complex environments such as solutions, interfaces, biological systems. Today, QM–MM-based computational spectroscopy methods constitute accomplished tools with refined predictive power. present review summarizes advances that made QM–MM UV–visible, Raman, IR, NMR, electron paramagnetic resonance, Mössbauer spectroscopies, providing every case introductory discussion corresponding methodological background. representative number are presented illustrate historical evolution state art this field, highlighting advantages limitations available methodologies. Finally, we our view perspectives open challenges field.

Язык: Английский

---

Applications of fluorescence spectroscopy in protein conformational changes and intermolecular contacts

BBA Advances, Год журнала: 2023, Номер 3, С. 100091 - 100091

Опубликована: Янв. 1, 2023

Emission fluorescence is one of the most versatile and powerful biophysical techniques used in several scientific subjects. It extensively applied studies proteins, their conformations, intermolecular contacts, such as protein-ligand protein-protein interactions, allowing qualitative, quantitative, structural data elucidation. This review, aimed to outline some widely this area, illustrate applications display a few examples. At first, on intrinsic proteins disclosed, mainly tryptophan side chain. Predominantly, research study protein conformational changes, changes intensities shifts emission maximums were discussed. Fluorescence anisotropy or polarization measurement changing orientation molecule space, concerning time between absorption events. Absorption indicate spatial alignment molecule's dipoles relative electric vector electromagnetic wave excitation emitted light, respectively. In other words, if fluorophore population excited with vertically polarized light will retain based how fast it rotates solution. Therefore, can be successfully interaction investigations. Then, green fluorescent (GFPs), photo-transformable (FPs) photoswitchable photoconvertible FPs, those Large Stokes Shift (LSS) are disclosed more detail. FPs potent tools for biological systems. Their versatility wide range colours properties allow many applications. Finally, application life sciences exposed, especially microscopy super-resolution that enables precise vivo photolabeling monitor movement interactions target proteins.

Язык: Английский

---

Biomimetic NIR-II fluorescent proteins created from chemogenic protein-seeking dyes for multicolor deep-tissue bioimaging

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 2, 2024

Abstract Near-infrared-I/II fluorescent proteins (NIR-I/II FPs) are crucial for in vivo imaging, yet the current NIR-I/II FPs face challenges including scarcity, requirement chromophore maturation, and limited emission wavelengths (typically < 800 nm). Here, we utilize synthetic protein-seeking NIR-II dyes as chromophores, which covalently bind to tag (e.g., human serum albumin, HSA) through a site-specific nucleophilic substitution reaction, thereby creating proof-of-concept biomimetic FPs. This chemogenic strategy can be accomplished under gentle physiological conditions without catalysis. Proteomics analysis identifies specific binding site (Cys 477 on DIII). significantly enhance brightness photostability, while improving biocompatibility, allowing high-performance lymphography angiography. is universal applicable wide range of spectrally separated real-time visualization multiple biological events. Overall, this straightforward approach holds potential transform protein-based bioimaging enables in-situ albumin targeting create deep-tissue imaging live organisms.

Язык: Английский

---