The Analyst, Год журнала: 2023, Номер 148(13), С. 2882 - 2891
Опубликована: Янв. 1, 2023
An overview of the principles and applications GFP-based biosensors.
Язык: Английский
Annual Review of Biochemistry, Год журнала: 2022, Номер 91(1), С. 571 - 598
Опубликована: Март 19, 2022
The Wnt pathway is central to a host of developmental and disease-related processes. remarkable conservation this intercellular signaling cascade throughout metazoan lineages indicates that it coevolved with multicellularity regulate the generation spatial arrangement distinct cell types. By regulating fate specification, mitotic activity, polarity, orchestrates development tissue homeostasis, its dysregulation implicated in defects, cancer, degenerative disorders. We review advances our understanding key pathway, from protein production secretion relay signal cytoplasm receiving cell. discuss evolutionary history as well endogenous synthetic modulators activity. Finally, we highlight remaining gaps knowledge transduction avenues for future research.
Язык: Английский
Процитировано
309
Drug Discovery Today, Год журнала: 2020, Номер 25(10), С. 1807 - 1821
Опубликована: Авг. 12, 2020
Язык: Английский
Процитировано
231
Chemical Society Reviews, Год журнала: 2023, Номер 52(4), С. 1189 - 1214
Опубликована: Янв. 1, 2023
The genetically encoded fluorescent sensors convert chemical and physical signals into light. They are powerful tools for the visualisation of physiological processes in living cells freely moving animals. protein is reporter module a biosensor. In this study, we first review history full emission spectra on structural basis. Then, discuss design Finally, briefly several major types biosensors that currently widely used based their molecular targets, which may be useful future biosensors.
Язык: Английский
Процитировано
75
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(3), С. 1391 - 1391
Опубликована: Янв. 23, 2024
Bioactive peptides, specific protein fragments with positive health effects, are gaining traction in drug development for advantages like enhanced penetration, low toxicity, and rapid clearance. This comprehensive review navigates the intricate landscape of peptide science, covering discovery to functional characterization. Beginning a peptidomic exploration natural sources, emphasizes search novel peptides. Extraction approaches, including enzymatic hydrolysis, microbial fermentation, specialized methods disulfide-linked extensively covered. Mass spectrometric analysis techniques data acquisition identification, such as liquid chromatography, capillary electrophoresis, untargeted analysis, bioinformatics, thoroughly outlined. The bioactivity incorporates various methodologies, from vitro assays silico techniques, advanced approaches phage display cell-based assays. also discusses structure–activity relationship context antimicrobial peptides (AMPs), ACE-inhibitory (ACEs), antioxidative (AOPs). Concluding key findings future research directions, this interdisciplinary serves reference, offering holistic understanding their potential therapeutic applications.
Язык: Английский
Процитировано
44
Biology of the Cell, Год журнала: 2025, Номер 117(2)
Опубликована: Фев. 1, 2025
Abstract Background Information The discovery of green fluorescent protein (GFP) and its derivatives has revolutionized cell biology. These proteins (FPs) have enabled the real‐time observation localization dynamics within live cells. Applications FP vary from monitoring gene/protein expression patterns, visualizing protein‐protein interactions, measuring stability, assessing mobility, creating biosensors. utility FPs also extends to biochemical approaches through immunoblotting proteomic analyses, aided by anti‐FP antibodies nanobodies. are notoriously robust with a tightly folded domain that confers strong stability relative resistance degradation denaturation. Methods Results In this study, we report various green, orange, red can be maintained in native, state during entire process sample extraction, incubation buffer, loading, migration on SDS‐PAGE (sodium dodecyl sulfate‐polyacrylamide gel electrophoresis) only minor adaptations traditional protocols. This protocol results ability detect quantify in‐gel fluorescence (IGF) endogenously‐expressed tagged directly after migration, using standard fluorescence‐imaging devices. approach eliminates need for chemiluminescent reagents, as well time‐consuming steps inherent such transfer onto membrane antibody incubations. Conclusions Significance Overall, IGF detection provides clearer data less background interference, sensitivity comparable or better than antibody‐based detection, quantification, broader dynamic range. After imaging, gels still used other applications total staining if needed. It expands possibilities allowing which not available. Our study explores feasibility, limitations, detecting endogenously expressed extracts, providing insights into preparation, imaging conditions, optimizations, potential co‐immunoprecipitation experiments.
Язык: Английский
Процитировано
2
Cell Reports, Год журнала: 2020, Номер 32(6), С. 108008 - 108008
Опубликована: Авг. 1, 2020
Язык: Английский
Процитировано
85
Nature Chemical Biology, Год журнала: 2021, Номер 17(5), С. 531 - 539
Опубликована: Фев. 1, 2021
Язык: Английский
Процитировано
62
Sensors, Год журнала: 2021, Номер 21(3), С. 906 - 906
Опубликована: Янв. 29, 2021
The purpose of this work is to provide an exhaustive overview the emerging biosensor technologies for detection analytes interest food, environment, security, and health. Over years, biosensors have acquired increasing importance in a wide range applications due synergistic studies various scientific disciplines, determining their great commercial potential revealing how nanotechnology biotechnology can be strictly connected. In present scenario, increased limit sensitivity unthinkable until few years ago. most widely used are optical-based devices such as surface plasmon resonance (SPR)-based fluorescence-based biosensors. Here, we will review them by highlighting progress design development could impact our daily life.
Язык: Английский
Процитировано
57
Cell, Год журнала: 2022, Номер 185(20), С. 3823 - 3837.e23
Опубликована: Сен. 1, 2022
Язык: Английский
Процитировано
48
Theranostics, Год журнала: 2024, Номер 14(6), С. 2464 - 2488
Опубликована: Янв. 1, 2024
Cancer has remained a formidable challenge in medicine and claimed an enormous number of lives worldwide.Theranostics, combining diagnostic methods with personalized therapeutic approaches, shows huge potential to advance the battle against cancer.This review aims provide overview theranostics oncology: exploring its history, current advances, challenges, prospects.We present fundamental evolution from radiotherapeutics, cellular therapeutics, nanotherapeutics, showcasing critical milestones last decade.From early concept targeted drug delivery emergence medicine, benefited advances imaging technologies, molecular biology, nanomedicine.Furthermore, we emphasize pertinent illustrations that revolutionary strategies cancer management enhance accuracy therapies customized for individual patients, thereby facilitating implementation medicine.Finally, describe future perspectives on emerging topics, field.
Язык: Английский
Процитировано
15