Capturing temporal variation in aquatic ecotoxicological risks: Chemical- versus effect-based assessment

The Science of The Total Environment, Год журнала: 2025, Номер 967, С. 178797 - 178797

Опубликована: Фев. 12, 2025

The integration of effect-based and chemical profiling has been advocated to assess the potential ecotoxicological risks posed by mixtures present in aquatic ecosystems. However, concentrations contaminants surface waters can vary greatly over time space, making it challenging ensure risk assessment. Although first results are promising, not yet proven that these combined approaches also capable capturing temporal variation risks. study aimed test this combining passive time-integrative sampling with chemical-analytical techniques agricultural waterways. Silicone rubber sheets polar organic integrative samplers (POCIS) were deployed four water bodies consecutive six-week periods. Passive sampler extracts analysed using a battery 22 vitro vivo bioassays tandem extensive target analysis 225 compounds. induced fluctuating bioassay responses for all locations during periods, highlighting presence spatial toxic pressure. A range compounds, primarily fungicides herbicides, detected periods at variable concentrations, persistent but pressure regions. toxicity observed could solely be attributed chemicals 6 % cases those explaining only 1-16.9 effects, indicating predominantly caused undetected chemicals. Risk assessments based on revealed frequent exceedances trigger values It is concluded better capture variations than traditional analyses, advanced needed explain bioanalytical response profiles.

Язык: Английский

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Chemical safety screening of products – better proactive

Journal of Chromatography A, Год журнала: 2025, Номер 1752, С. 465946 - 465946

Опубликована: Апрель 19, 2025

The increasing pressure to ensure product safety in a global market comes up against the current practice of targeting only known hazardous compounds analysis. However, refers not but also unknown or hidden hazards that are very important know and avoid. Shortcomings limitations currently used technologies seem cause an obvious discrepancy between intended actual consumer protection. Products as safe claimed by stakeholders. An existing overlooked proactive screening with prioritization strategy is brought into focus it offers unique solution. It can handle complexity thousands identity toxicity figure out compounds, both unknown. Using hardly any sample preparation effect detection at early position workflow game changer overlook compounds. All analytical needed, key re-arrangement instrument order, i.e. firstly hazard-related (effect first) secondly, on identification prioritized Such revealed previously products be safe. highly sustainable, affordable, all-in-one 2LabsToGo-Eco easy-to-use planar bioassays empowers stakeholders implement dynamic risk management. transition greater efficacy protection needs incentives critical review aims stimulate debate.

Язык: Английский

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Exploring the Concepts of Concentration Addition and Independent Action Using a Linear Low‐Effect Mixture Model

Environmental Toxicology and Chemistry, Год журнала: 2020, Номер 39(12), С. 2552 - 2559

Опубликована: Сен. 25, 2020

Chemicals emitted into the environment are typically present at low concentrations but may act together in mixtures. Concentration-response curves of vitro bioassays were often linear for effect levels <30%, and predictions concentration addition (CA) similarly acting chemicals independent action (IA) dissimilarly overlapped. We derived a joint CA/IA mixture model low-effect level portion concentration-response curves. In first case study, we evaluated cytotoxicity over 200 mixtures up to 17 components that mixed ratios as they occurred river water. The full IA indistinguishable from CA 10% effect, confirming applicability levels. second if environmental trigger effects enough apply. detected scaled by their toxic potencies estimate complex mixture. 86% 156 samples fell validity range (<10% level), assumption unit summation. is not suitable testing specific hypotheses interactions mixtures, where more refined models required; it helpful interpretation (multicomponent) especially water with relatively level. Environ Toxicol Chem 2020;39:2552-2559. © 2020 Authors. Environmental Toxicology Chemistry published Wiley Periodicals LLC on behalf SETAC.

Язык: Английский

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Wastewater treatment efficacy evaluated with in vitro bioassays

Water Research X, Год журнала: 2020, Номер 9, С. 100072 - 100072

Опубликована: Окт. 1, 2020

Bioassays show promise as a complementary approach to chemical analysis assess the efficacy of wastewater treatment processes they can detect mixture effects all bioactive chemicals in sample. We investigated ten Australian plants (WWTPs) covering 42% national population over seven consecutive days. Solid-phase extracts influent and effluent were subjected an vitro test battery with six bioassays nine endpoints that captured major modes action detected receiving surface waters. WWTP influents effluents compared on basis population- flow-normalised effect loads, which provided insights into biological exhibited by before after treatment. Effect removal varied between depended process. An ozonation step had best efficacy, while WWTPs only primary resulted poor effects. was generally better for estrogenic peroxisome proliferator-activated receptor than inhibition photosynthesis, is consistent persistence herbicides causing this effect. Cytotoxicity oxidative stress response sum parameter including transformation products poorer specific except photosynthesis inhibition. Although more 500 analysed, explained typically less 10% measured effect, apart from algal toxicity, where majority could be one dominant herbicide, diuron. Overall, current study demonstrated utility applying alongside evaluate loads pollution reaching efficacy.

Язык: Английский

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Effect‐Based Trigger Values for Mixtures of Chemicals in Surface Water Detected with In Vitro Bioassays

Environmental Toxicology and Chemistry, Год журнала: 2020, Номер 40(2), С. 487 - 499

Опубликована: Ноя. 30, 2020

Abstract Effect‐based trigger (EBT) values for in vitro bioassays are important surface water quality monitoring because they define the threshold between acceptable and poor quality. They have been derived highly specific bioassays, such as hormone‐receptor activation reporter gene by reading across from existing chemical guideline values. This read‐across method is not easily applicable to indicative of adaptive stress responses, which triggered many different chemicals, nuclear receptors xenobiotic metabolism, chemicals bind with rather low specificity. We propose an alternative approach EBT distribution specificity ratios all active chemicals. The ratio predicted baseline toxicity a given bioassay its measured endpoint. Unlike previous methods derive EBTs, proposed accounts mixture effects includes only high‐potency EBTs were cytotoxicity that was defined equivalent 1% native sample. scaled median log‐normal each bioassay. illustrate new using example AREc32 assay, oxidative response, 2 receptor assays targeting peroxisome proliferator–activated gamma arylhydrocarbon receptor. less conservative than previously but able differentiate untreated insufficiently treated wastewater treatment plant effluent secondary or tertiary water. Environ Toxicol Chem 2021;40:487–499. © 2020 Authors. Environmental Toxicology Chemistry published Wiley Periodicals LLC on behalf SETAC.

Язык: Английский

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Removal of micropollutants and biological effects by conventional and intensified constructed wetlands treating municipal wastewater

Water Research, Год журнала: 2021, Номер 201, С. 117349 - 117349

Опубликована: Июнь 12, 2021

Seven treatment wetlands and a municipal wastewater plant (WWTP) were weekly monitored over the course of one year for removal conventional parameters, selected micropollutants (caffeine, ibuprofen, naproxen, benzotriazole, diclofenac, acesulfame, carbamazepine) biological effects. The wetland designs investigated include horizontal subsurface flow (HF) variety with intensification (aeration, two-stages, or reciprocating flow). Complementary to common approach analyzing individual chemicals, in vitro bioassays can detect toxicity mixture known unknown components given water sample. A panel five cell-based reporter gene was cover environmentally relevant endpoints (AhR: indicative activation aryl hydrocarbon receptor; PPARγ: binding peroxisome proliferator-activated receptor gamma; ERα: estrogen alpha; GR: glucocorticoid oxidative stress response). While carbamazepine persistent intensified wetlands, mean monthly mass up 51% achieved HF wetland. two-stage system showed highest efficacy all effects (91% >99%). ranged from 56% 77% 60% 99% WWTP. Bioanalytical equivalent concentrations (BEQs) AhR, PPARγ, response often below recommended effect-based trigger (EBT) values surface water, indicating great benefit using nature-based solutions treatment. Intensified remove both more efficiently than (non-aerated) some cases,

Язык: Английский

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Effect‐Based Trigger Values Are Essential for the Uptake of Effect‐Based Methods in Water Safety Planning

Environmental Toxicology and Chemistry, Год журнала: 2022, Номер 42(3), С. 714 - 726

Опубликована: Дек. 16, 2022

Effect-based methods (EBMs) using in vitro bioassays and well plate-based vivo assays are recommended for water quality monitoring because they can capture the mixture effects of many chemicals present water. Many highly sensitive, so an effect a bioassay does not necessarily indicate poor chemical quality. Consequently, effect-based trigger values (EBTs) have been introduced to differentiate between acceptable unacceptable required wider acceptance EBMs by sector regulatory bodies. These EBTs derived both drinking surface protect human ecological health, respectively, available indicative specific receptor-mediated effects, as adaptive stress responses, apical triggered chemicals. An overview currently is provided, simple approach proposed predict interim without EBT based on concentration assay reference compound. There was good agreement predicted this simplistic from literature more robust methods. Finally, interpretation framework that outlines steps take if sample exceeds developed help facilitate uptake routine safety planning production. Environ Toxicol Chem 2023;42:714-726. © 2022 The Authors. Environmental Toxicology Chemistry published Wiley Periodicals LLC behalf SETAC.

Язык: Английский

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Non-target screening of micropollutants and transformation products for assessing AOP-BAC treatment in groundwater

Environmental Pollution, Год журнала: 2022, Номер 309, С. 119758 - 119758

Опубликована: Июль 11, 2022

Standard monitoring programs give limited insight into groundwater status, especially transformation products (TPs) formed by natural processes or advanced oxidation (AOP), are normally underrepresented. In this study, using suspect and non-target screening, we performed a comprehensive analysis of before after AOP UV/H2O2 consecutively installed biological activated carbon filters (BAC). By up to 413 compounds were detected in the groundwater, with an average 70% removal AOP. However, similar number during process, shown from three waterworks. The most polar typically stable A subsequent BAC filter showed 95% TPs, but only 46% remaining precursors. for was highly dependent on acidic basic functional groups molecules. 49 wide polarity range could be identified supercritical fluid chromatography (SFC) liquid (LC) high resolution mass spectrometry (HRMS); these, 29 already present groundwater. To best our knowledge, five have never been reported (4-chlorobenzenesulfonic acid, dibutylamine, N-phenlybenzenesulfonamide, 2-(methylthio)benzothiazole benzothiazole-2-sulfonate). further rarely first time Danish (2,4,6-trichlorophenol, 2,5-dichlorobenzenesulfonic trifluormethansulfonic pyrimidinol benzymethylamine). Twenty AOP, which 10 All related agricultural industrial as well artificial sweeteners. Whereas dechlorination common degradation pathway chlorophenols, (ultra-) short chain PFAs no study. We prioritized 11 concern, however, toxicity many remains unknown, TPs.

Язык: Английский

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Chemical Mixtures and Multiple Stressors: Same but Different?

Environmental Toxicology and Chemistry, Год журнала: 2023, Номер 42(9), С. 1915 - 1936

Опубликована: Апрель 10, 2023

Ecosystems are strongly influenced by multiple anthropogenic stressors, including a wide range of chemicals and their mixtures. Studies on the effects stressors have largely focussed nonchemical whereas studies chemical mixtures ignored other stressors. However, both research areas face similar challenges require tools methods to predict joint or frameworks integrate missing. We provide an overview paradigms, tools, commonly used in stressor mixture discuss potential domains cross-fertilization challenges. First, we compare general paradigms ecotoxicology (applied) ecology explain historical divide. Subsequently, approaches for identification interactions, characterization, designing experiments. suggest that too focused interactions would benefit from integration regarding null model selection. Stressor characterization is typically more costly While comprehensive classification systems at suborganismal level been developed, recent account environmental context. Both suffer rather simplified experimental designs focus only limited number chemicals, treatments. concepts can guide realistic capturing spatiotemporal dynamics. process-based data-driven models particularly promising tackle challenge prediction (meta-)communities (meta-)food webs. propose framework assessment Environ Toxicol Chem 2023;42:1915-1936. © 2023 The Authors. Environmental Toxicology Chemistry published Wiley Periodicals LLC behalf SETAC.

Язык: Английский

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Bioanalytical and chemical characterization of organic micropollutant mixtures in long-term exposed passive samplers from the Joint Danube Survey 4: Setting a baseline for water quality monitoring

Environment International, Год журнала: 2023, Номер 178, С. 107957 - 107957

Опубликована: Май 11, 2023

Monitoring methodologies reflecting the long-term quality and contamination of surface waters are needed to obtain a representative picture pollution identify risk drivers. This study sets baseline for characterizing chemical in Danube River using an innovative approach, combining continuous three-months use passive sampling technology with comprehensive (747 chemicals) bioanalytical (seven vitro bioassays) assessment during Joint Survey (JDS4). is one world's largest investigative surface-water monitoring efforts longest river European Union, which water after riverbank filtration broadly used drinking production. Two types samplers, silicone rubber (SR) sheets hydrophobic compounds AttractSPETM HLB disks hydrophilic compounds, were deployed at nine sites approximately 100 days. The was dominated by industrial SR samplers together pharmaceuticals personal care products samplers. Comparison Estimated Environmental Concentrations Predicted No-Effect revealed that studied sites, least 4-7 (HLB) compound(s) exceeded quotient 1. We also detected AhR-mediated activity, oxidative stress response, peroxisome proliferator-activated receptor gamma-mediated estrogenic, androgenic, anti-androgenic activities bioassays. A significant portion estrogenic could be explained analytes several while other bioassays much activity remained unexplained. effect-based trigger values some sites. identified drivers mixture effects deserve further attention ecotoxicological environmental research. novel approach provides benchmark effect potentials mixtures future large bodies.

Язык: Английский

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