Environmental Pollution,
Год журнала:
2023,
Номер
330, С. 121818 - 121818
Опубликована: Май 12, 2023
Bisphenol
(BP)
structural
analogues
of
BPA
are
widely
used.
Previous
studies
showed
similar
effects
and
BPS
on
reproduction
in
several
species
including
human.
We
hypothesised
that
the
bisphenols
(BPs)
could
accumulate
granulosa
cells
(GCs)
affects
steroidogenesis.
This
study
investigated
seven
BP
their
equimolar
cocktail
human
(hGC)
assessed
BPA,
BPS,
BPF
BPAF
level
exposures
follicular
fluid
277
women
undergoing
Assisted
Reproductive
Technology.
The
hGCs
were
recovered
after
oocyte
punctures
treated
with
(BPS,
BPAF,
BPF,
BPAP,
BPE
BPB)
or
7
×
1.43
7.14
μM
for
each
BPs,
sum
BPs
reaching
10
("∑BPs
μM"),
50
respectively.
Oestradiol
progesterone
secretion,
cell
proliferation,
viability
expression
steroidogenic
enzymes
investigated.
Progesterone
secretion
was
decreased
by
6
"∑BPs
μM",
(-17.8
to
-41.3%)
all
μM"
(-21.8
-84.2%).
only
BPAP
(-37.8%
-44%,
respectively),
corresponding
decreases
CYP17A1
CYP19A1
gene
expression.
Cellular
proliferation
treatment
(-32.2%),
(-29%),
BPB
(-24%)
(-33.1%).
(50
μM)
increased
HSD3B2
mRNA
At
least
one
detected
64
(23.1%)
fluids.
Similar
observed
and/or
suggesting
cumulative
BPs.
Our
results
highlight
urge
consider
simultaneously
further
investigate
potential
additive
synergistic
Environment International,
Год журнала:
2023,
Номер
175, С. 107949 - 107949
Опубликована: Апрель 25, 2023
Despite
being
one
of
the
most
world's
widely
used
and
mass-produced
compounds,
bisphenol
A
(BPA)
has
a
wide
range
toxic
effects.
Bisphenol
P
(BPP),
an
alternative
to
BPA,
been
detected
in
many
foods.
The
effects
BPP
dietary
exposure
on
gut
microbiota
intestinal
barrier
were
unclear.
We
designed
three
batches
animal
experiments:
first
studied
mice
exposed
(30
µg/kg
BW/day)
for
nine
weeks
found
that
they
gained
weight
developed
dysbiosis
microbiota.
second,
using
typical
human
levels
(L,
0.3
BW/day
BPP)
higher
concentrations
(M,
30
BPP;
H,
3000
BPP),
caused
mice,
activated
Lipopolysaccharide
(LPS)
/TLR4/NF-κB
signaling
pathway,
triggered
inflammatory
response,
increased
permeability,
promoted
bacterial
translocation
leading
disruption.
third
treatment
combination
antibiotics
alleviated
inflammation
injury.
This
study
demonstrated
mechanism
injury
concentration
damage
by
exposure,
providing
reference
data
use
control
yielding
new
insights
disease
prevention.
