Environmental Science & Technology,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 25, 2024
Antibiotic
resistance
poses
a
significant
threat
to
human
health.
While
most
studies
focus
on
bacteria,
interactions
between
antibiotics
and
other
crucial
microbial
groups
like
protists
remain
uncertain.
This
study
investigates
how
interact
with
examines
these
impact
the
fate
of
genes.
It
reveals
that
amoebae
exhibit
high
eight
high-risk
environmental
antibiotics,
accumulating
quantities
within
their
cells.
Wild
amoeboid
strains
from
distant
locations
carry
substantial
antibiotic
genes
(ARGs)
metal
(MRGs),
heterogeneity
single
species.
Amoeboid
symbionts
pathogens
predominantly
Paraburkholderia
have
reduced
genomes
fewer
compared
free-living
strains,
while
amoeba-endogenous
Stenotrophomonas
maltophilia
does
not
significantly
genome
size.
suggests
hosts
serve
as
temporary
medium
facilitating
its
transmission.
In
summary,
unveils
soil
represent
unexpected
hotspots
for
Future
research
should
assess
effects
often-overlooked
explore
role
in
spreading
ARGs
MRGs
ecosystems.
Incorporating
into
broader
is
recommended,
highlighting
significance
One
Health
perspective.
Environment International,
Год журнала:
2024,
Номер
190, С. 108915 - 108915
Опубликована: Июль 28, 2024
Pathogenic
microorganisms
with
antibiotic
resistance
genes
(ARGs)
pose
a
serious
threat
to
public
health
and
soil
ecology.
Although
new
drugs
available
antibacterial
materials
can
kill
ARG
carriers
but
accidentally
beneficial
microorganisms.
Therefore,
the
rapid
enrichment
separation
of
ARGs
their
from
is
becoming
an
important
strategy
for
controlling
diffusion
ARGs.
Hydroxamate
siderophore
(HDS)
has
gained
widespread
attentions
its
involvement
in
trace
element
transfer
among
environment,
we
thus
explored
in-situ
trapping-enrichment
method
via
small
molecular
HDS
secreted
by
Pseudomonas
fluorescens
HMP01.
In
this
study,
demonstrate
that
significantly
traps
enriches
certain
ARGs,
including
chloramphenicol,
MLS,
rifamycin,
tetracycline
environment.
The
efficiencies
were
1473-fold,
38-fold,
17-fold,
5-fold,
respectively,
higher
than
those
control
group.
Specifically,
primary
enriched
rpoB,
mphL,
catB2,
tetA(60),
Bacillus,
Rhizobium,
Rossellomorea,
Agrobacterium
hosts
these
This
was
caused
upregulation
chemotaxis
(e.g.,
cheW,
cheC,
cheD)
biofilm
formation
within
bacterial
population.
Notably,
representative
such
as
cat,
macB,
rpoB
reduced
36%,
85.7%,
72%,
paddy
after
enrichment.
Our
research
sheds
light
on
potential
application
rapping
agent
eco-friendly
reduction
environments.
Environmental Science & Technology,
Год журнала:
2023,
Номер
57(9), С. 3590 - 3601
Опубликована: Фев. 22, 2023
Protist
predation
is
a
crucial
biotic
driver
modulating
bacterial
populations
and
functional
traits.
Previous
studies
using
pure
cultures
have
demonstrated
that
bacteria
with
copper
(Cu)
resistance
exhibited
fitness
advantages
over
Cu-sensitive
under
the
pressure
of
protist
predation.
However,
impact
diverse
natural
communities
grazers
on
Cu
in
environments
remains
unknown.
Here,
we
characterized
phagotrophic
protists
long-term
Cu-contaminated
soils
deciphered
their
potential
ecological
impacts
resistance.
Long-term
field
pollution
increased
relative
abundances
most
lineages
Cercozoa
Amoebozoa
but
reduced
abundance
Ciliophora.
After
accounting
for
soil
properties
pollution,
phagotrophs
were
consistently
identified
as
important
predictor
Cu-resistant
(CuR)
community.
Phagotrophs
positively
contributed
to
gene
(copA)
through
influencing
cumulative
-sensitive
clusters.
Microcosm
experiments
further
confirmed
promotion
effect
Our
results
indicate
selection
by
can
strong
CuR
community,
which
broadens
our
understanding
function
protists.
Ecology and Evolution,
Год журнала:
2023,
Номер
13(3)
Опубликована: Март 1, 2023
Amoebae
are
one
major
group
of
protists
that
widely
found
in
natural
and
engineered
environments.
They
a
significant
threat
to
human
health
not
only
because
many
them
pathogenic
but
also
due
their
unique
role
as
an
environmental
shelter
for
pathogens.
However,
unsolved
issue
the
amoeba-bacteria
relationship
is
why
so
bacteria
live
within
amoeba
hosts
while
they
can
independently
By
using
facultative
amoeba-
Environmental Science & Technology,
Год журнала:
2023,
Номер
57(15), С. 6108 - 6118
Опубликована: Апрель 7, 2023
Drinking
water
systems
host
a
wide
range
of
microorganisms
essential
for
biosafety.
However,
one
major
group
waterborne
pathogens,
protozoa,
is
relatively
neglected
compared
to
bacteria
and
other
microorganisms.
Until
now,
little
known
about
the
growth
fate
protozoa
their
associated
in
drinking
systems.
In
this
study,
we
aim
investigate
how
treatment
affects
subtropical
megacity.
The
results
showed
that
viable
were
prevalent
city's
tap
water,
amoebae
component
protozoa.
addition,
protozoan-associated
contained
many
potential
pathogens
primarily
enriched
amoeba
hosts.
Furthermore,
study
current
disinfection
methods
have
effect
on
bacteria.
Besides,
ultrafiltration
membranes
unexpectedly
served
as
an
ideal
surface
systems,
they
could
significantly
promote
amoeba-associated
conclusion,
shows
are
which
may
present
emerging
health
risk
Environmental Science & Technology,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 3, 2024
Heterogeneous
hydrogenation
is
surging
as
a
promising
strategy
for
selective
removal
of
water
pollutants,
yet
numerous
efforts
rely
on
catalyst
design
to
advance
catalytic
activity.
Herein,
we
enhanced
the
mass
transfer
and
utilization
hydrogen
reagent
through
construction
two-phase
flow-through
membrane
reaction
device
(Pd/SiC-MR).
Pd/SiC-MR
displays
high
efficiency
selectivity
toward
multiple
pollutants.
For
instance,
rapid
(∼0.35
s)
exclusive
(>99%)
carbon-chlorine
bond
in
organohalogens
were
realized
at
flux
(220
L/m
Journal of Hazardous Materials,
Год журнала:
2024,
Номер
480, С. 136020 - 136020
Опубликована: Окт. 5, 2024
The
anaerobic
oxidation
of
methane
(AOM)
carried
out
by
methanotrophic
archaea
(ANME)
plays
an
important
role
in
mitigating
emissions
from
aqueous
environments
and
has
applications
bioremediation
wastewater
treatment.
Previous
studies
showed
that
AOM
could
be
coupled
to
chromate
reduction.
However,
the
specific
responsible
microorganisms
biochemical
mechanisms
are
unclear.
Herein,
we
a
consortium
dominated
ANME
"Candidatus
Methanoperedens"
was
able
couple
reduction
Cr(VI)
Cr(III)
at
stoichiometry
close
theoretical
ratio.
Quantitative
distribution
analysis
products
suggested
predominantly
reduced
via
extracellular
respiratory
pathways.
Further
Cr(III)-targeted
fluorescent
visualization
combined
with
single-cell
electron
microscopic
imaging
"Ca.
independently.
Biochemical
mechanism
investigation
proteomic
proteins
for
nitrate
under
nitrate-reducing
conditions
were
significantly
downregulated
Cr(VI)-reducing
incubation.
Instead,
many
multiheme
cytochrome
c
(MHCs)
among
most
upregulated
during
process,
suggesting
MHC-governed
pathways
significant
upregulation
formate-dependent
nitrite
reductase
indicated
its
potential
contribution
small
proportion
inside
cells.
Water Biology and Security,
Год журнала:
2022,
Номер
1(3), С. 100057 - 100057
Опубликована: Июнь 14, 2022
Amoeboid
protists,
an
assemblage
of
organisms
belonging
to
different
phylogenetic
lineages,
have
drawn
increasing
attention
due
their
crucial
ecological
roles
in
various
environments
and
potential
health
risks.
Currently,
18S
rRNA
gene
sequencing
is
widely
applied
for
the
detection
amoebae.
However,
it
not
clear
which
best
primer
pair
amplification
This
study
compared
four
most
commonly
used
pairs
revealing
diversity,
composition,
core
species,
community
assembly
processes
amoebae
water
sediments.
We
found
that
choice
primers
artificially
influences
composition
also
short-read
fragments
may
lead
mismatches
taxonomy
were
suitable
analyses.
In
contrast,
full-length
could
detect
highest
number
amoeba
lineages
annotate
80%
reads
known
species.
did
as
many
species
V4
primers.
Moreover,
we
showed
beta
diversity
determination
largely
unaffected
by
choice,
but
influence
our
interpretations
process
underlying
stochasticity
determinism.
indicates
read
region
Illumina
are
profiling
environment.
