Omics-based high throughput toxicity screening and risk assessment of pollutants DOI

Lu Yan,

Xiao Gou, Pu Xia

и другие.

Chinese Science Bulletin (Chinese Version), Год журнала: 2022, Номер 67(35), С. 4159 - 4169

Опубликована: Окт. 13, 2022

Screening priority pollutants with high toxicity and risk potential from a large number of environmental chemical is the prerequisite for prevention control risks. After entering living system, toxic can induce adverse outcomes through series disturbed molecular targets biological pathways. Existing high-throughput screening methods rely on few isolated cellular or endpoints, making it difficult to capture key processes that cause determine direct relationship between disease. Meanwhile, awareness increasing we are exposed true cocktail chemicals, among which only fraction has been identified analytical methods. Integrated bioanalytical approaches, such as effect-directed analysis identification evaluation (TIE), have used identify drivers in complex mixture pollutants. However, still elucidate toxicological stress environment. Toxicogenomics take advantage genome-wide coverage, throughput, big data capacity associated omics technologies achieve technology innovation assessment We reviewed technical advantages recent progress toxicogenomic methodology four aspects specific research cases. To events initiated by pollutants, toxicogenomics could uncover pathways potentially at early development stage toxicity. Dose-dependent transcriptomes testing developed quantitatively assess responses induced level, improve sensitivity identifying bioactivity 1–3 orders magnitude. health outcome systemic strategies provide mechanistic information pollution multiple levels. Through pathway enrichment analysis, gene ontology (GO) kyoto encyclopedia genes genomes (KEGG) pathway, be captured. Moreover, multi-omics integration allows multi-scale, comprehensive network systematic understanding (AOPs) guide ecological human assessments. For diseases diagnostics, functional genomics new tool explore mechanisms individual disease susceptibility under exposure. Variations toxicant-induced largely due genetic across different populations. The established combining epidemiological approaches. Finally, management important overall effects pool pollutant. combination bioassays highly bioactive screen pollutant pose Recently, progresses made integrating transcriptomic techniques (mTIE) mixtures distinguish inducing corresponding responses. Particularly, dose-response transcriptome evaluate toxicant resolution. In summary, omics-based will strongly support accurate conventional future.

Язык: Английский

Identification and Prioritization of Environmental Organic Pollutants: From an Analytical and Toxicological Perspective DOI
Ting Ruan, Pengyang Li, Haotian Wang

и другие.

Chemical Reviews, Год журнала: 2023, Номер 123(17), С. 10584 - 10640

Опубликована: Авг. 2, 2023

Exposure to environmental organic pollutants has triggered significant ecological impacts and adverse health outcomes, which have been received substantial increasing attention. The contribution of unidentified chemical components is considered as the most knowledge gap in understanding combined effects pollutant mixtures. To address this issue, remarkable analytical breakthroughs recently made. In review, basic principles on recognition are overviewed. Complementary methodologies (i.e., quantitative structure–activity relationship prediction, mass spectrometric nontarget screening, effect-directed analysis) experimental platforms briefly described. stages technique development and/or essential parts workflow for each then reviewed. Finally, plausible paths applications future screening methods, interdisciplinary techniques achieving toxicant identification, burgeoning strategies risk assessment cocktails discussed.

Язык: Английский

Процитировано

88
Unravelling High-Molecular-Weight DBP Toxicity Drivers in Chlorinated and Chloraminated Drinking Water: Effect-Directed Analysis of Molecular Weight Fractions DOI
Huiyu Dong, Amy A. Cuthbertson, Michael J. Plewa

и другие.

Environmental Science & Technology, Год журнала: 2023, Номер 57(47), С. 18788 - 18800

Опубликована: Июль 7, 2023

As disinfection byproducts (DBPs) are ubiquitous sources of chemical exposure in disinfected drinking water, identifying unknown DBPs, especially drivers toxicity, is one the major challenges safe supply water. While >700 low-molecular-weight DBPs have been identified, molecular composition high-molecular-weight remains poorly understood. Moreover, due to absence standards for most it difficult assess toxicity contributions new identified. Based on effect-directed analysis, this study combined predictive cytotoxicity and quantitative genotoxicity analyses Fourier transform ion cyclotron resonance mass spectrometry (21 T FT-ICR-MS) identification resolve weight fractions that induce chloraminated chlorinated waters, along with these DBP drivers. Fractionation using ultrafiltration membranes allowed investigation <1 kD, 1-3 3-5 >5 kD fractions. Thiol reactivity based single-cell gel electrophoresis assays revealed fraction both waters exhibited highest levels direct genotoxicity. The target was used subsequent identification. Ultrahigh-resolution MS identified singly charged species (as evidenced by 1 Da spacing 13C isotopologues), including 3599 chlorine-containing empirical formulas CHOCl, CHOCl2, CHOCl3, a relative abundance order CHOCl > CHOCl2 ≫ CHOCl3. Interestingly, more CHOCl1-3 were vs waters. This may be slower reactions NH2Cl. Most formed composed Cl-DBPs (up kD) rather than known DBPs. increase chlorine number detected, O/C ratio an increasing trend, while modified aromaticity index (AImod) showed opposite trend. In water treatment processes, removal natural organic matter high AImod value should strengthened minimize formation

Язык: Английский

Процитировано

39
Progress, applications, and challenges in high-throughput effect-directed analysis for toxicity driver identification — is it time for HT-EDA? DOI Creative Commons
Iker Alvarez-Mora, Katarzyna Arturi, Frederic Béen

и другие.

Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Июль 12, 2024

Abstract The rapid increase in the production and global use of chemicals their mixtures has raised concerns about potential impact on human environmental health. With advances analytical techniques, particular, high-resolution mass spectrometry (HRMS), thousands compounds transformation products with adverse effects can now be detected samples. However, identifying prioritizing toxicity drivers among these remain a significant challenge. Effect-directed analysis (EDA) emerged as an important tool to address this challenge, combining biotesting, sample fractionation, chemical unravel complex mixtures. Traditional EDA workflows are labor-intensive time-consuming, hindering large-scale applications. concept high-throughput (HT) recently gained traction means accelerating workflows. Key features HT-EDA include combination microfractionation downscaled bioassays, automation preparation efficient data processing supported by novel computational tools. In addition microplate-based high-performance thin-layer chromatography (HPTLC) offers interesting alternative HPLC HT-EDA. This review provides updated perspective state-of-the-art HT-EDA, methods/tools that incorporated into It also discusses recent studies HT prioritization tools, along considerations regarding HPTLC. By current gaps proposing new approaches overcome them, aims bring step closer monitoring Graphical

Язык: Английский

Процитировано

10
Endocrine disrupting effect and reproductive toxicity of the separate exposure and co-exposure of nano-polystyrene and diethylstilbestrol to zebrafish DOI
Xinyu Lin, Yuanning Wang, Xianhai Yang

и другие.

The Science of The Total Environment, Год журнала: 2022, Номер 865, С. 161100 - 161100

Опубликована: Дек. 23, 2022

Язык: Английский

Процитировано

35
Niclosamide Exposure at Environmentally Relevant Concentrations Efficaciously Inhibited the Growth and Disturbed the Liver-Gut Axis of Adult Male Zebrafish DOI
Biran Zhu, Lei Lei, Yumiao Sun

и другие.

Environmental Science & Technology, Год журнала: 2022, Номер 56(16), С. 11516 - 11526

Опубликована: Июль 28, 2022

In the current study, adult male zebrafish fed a normal diet (ND) or high-fat (HFD) were exposed to niclosamide (NIC) at environmentally relevant concentrations reveal accumulation and distribution in different tissues evaluate effects on liver-gut axis. Chemical analysis indicated that liver bore greater burden of NIC compared with brain gonads zebrafish, HFD-fed fish their than those ND-fed fish. The indications from body weight, growth rate, mass index, micro-CT images, biochemical pathological changes confirmed can efficaciously curb weight gain improve overloads plasma insulin glucose zebrafish. However, potential axis also elucidated: disturbed mitochondrial energy production, inhibited glycemic triacylglycerol biosynthesis but promoted free fatty acid catabolism, therefore reduced lipid hepatocytes; impaired physical barrier, evoked inflammatory oxidative stress led microbiota dysbiosis intestine. There findings highlighted necessity for evaluating its impacts health wild animals as well human beings upon long-term exposure.

Язык: Английский

Процитировано

31
Identify organic contaminants of high-concern based on non-targeted toxicity testing and non-targeted LC-HRMS analysis in tap water and source water along the Yangtze River DOI
Shaoqing Zhang, Liben Chou,

Wenxuan Zhu

и другие.

Water Research, Год журнала: 2024, Номер 253, С. 121303 - 121303

Опубликована: Фев. 10, 2024

Язык: Английский

Процитировано

9
High-Efficiency Effect-Directed Analysis Leveraging Five High Level Advancements: A Critical Review DOI
Jifu Liu, Tongtong Xiang, Xue‐Chao Song

и другие.

Environmental Science & Technology, Год журнала: 2024, Номер 58(23), С. 9925 - 9944

Опубликована: Май 31, 2024

Organic contaminants are ubiquitous in the environment, with mounting evidence unequivocally connecting them to aquatic toxicity, illness, and increased mortality, underscoring their substantial impacts on ecological security environmental health. The intricate composition of sample mixtures uncertain physicochemical features potential toxic substances pose challenges identify key toxicants samples. Effect-directed analysis (EDA), establishing a connection between found samples associated hazards, enables identification that can streamline research efforts inform management action. Nevertheless, advancement EDA is constrained by following factors: inadequate extraction fractionation samples, limited bioassay endpoints unknown linkage higher order impacts, coverage chemical (i.e., high-resolution mass spectrometry, HRMS), lacking effective bioassays analysis. This review proposes five advancements enhance efficiency addressing these challenges: (1) multiple adsorbents for comprehensive extraction, (2) microfractionation multidimensional refined fractionation, (3) robust vivo/vitro omics, (4) high-performance configurations HRMS analysis, (5) chemical-, data-, knowledge-driven approaches streamlined toxicant validation. We envision future will integrate big data artificial intelligence based development quantitative cutting-edge microfractionation, ultraperformance MS hazard factors, serving broader governance.

Язык: Английский

Процитировано

6
Effect-directed analysis and beyond: how to find causal environmental toxicants DOI Creative Commons
Zhenyu Tian, Madison H. McMinn, Mingliang Fang

и другие.

Exposome, Год журнала: 2023, Номер 3(1)

Опубликована: Янв. 1, 2023

Abstract Humans and wildlife are exposed to complex environmental mixtures. Identifying causal toxic pollutants in samples remains challenging because of the high complexity sample mixtures unknown nature potential toxicants. In field chemistry toxicology, this pursuit toxicants leads us method effect-directed analysis (EDA), an integrated comprised three iterative modules: (1) bioassays guide component prioritization; (2) fractionation reduce mixture complexity; (3) chemical identify commentary review, we try provide a concise guideline for EDA beginners by summarizing good practices from successful studies, categorized sample-toxicity pair selection, efficient separation, analysis. We also discussed practical challenges faced with current practices. Based on these above, suggestions perspectives future studies. Specifically, applying human biological examples causes diseases. proposed collaboration between chemists toxicologists, health scientists, epidemiologists, physicians, social scientists.

Язык: Английский

Процитировано

13
High-efficiency effect-directed analysis (EDA) advancing toxicant identification in aquatic environments: Latest progress and application status DOI Creative Commons

Wenrui Luo,

Liben Chou,

Qinglan Cui

и другие.

Environment International, Год журнала: 2024, Номер 190, С. 108855 - 108855

Опубликована: Июнь 28, 2024

Facing the great threats to ecosystems and human health posed by continuous release of chemicals into aquatic environments, effect-directed analysis (EDA) has emerged as a powerful tool for identifying causative toxicants. However, traditional EDA shows problems low-coverage, labor-intensive low-efficiency. Currently, number high-efficiency techniques have been integrated improve toxicant identification. In this review, latest progress current limitations EDA, comprising high-coverage effect evaluation, high-resolution fractionation, chemical analysis, high-automation peak extraction structure elucidation, are summarized. Specifically, data processing algorithms in silico elucidation well developed enhance EDA. While evaluation should be further emphasized, especially omics tools data-independent mass acquisition. For application status is widely applied surface water wastewater. Estrogenic, androgenic aryl hydrocarbon receptor-mediated activities most concerning, with toxicants showing typical structural features steroids benzenoids. A better understanding would beneficial advance field greatly support environment monitoring.

Язык: Английский

Процитировано

5
Pioneering an Effect-Based Early Warning System for Hazardous Chemicals in the Environment DOI Creative Commons
Georgios Niarchos, ‪Nikiforos Alygizakis, Mario Carere

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 180, С. 117901 - 117901

Опубликована: Авг. 5, 2024

Existing regulatory frameworks often prove inadequate in identifying contaminants of emerging concern (CECs) and determining their impacts on biological systems at an early stage. The establishment Early Warning Systems (EWSs) for CECs is becoming increasingly relevant policy-making, aiming to proactively detect chemical hazards implement effective mitigation measures. Effect-based methodologies, including bioassays effect-directed analysis (EDA), offer valuable input EWSs with a view pinpointing the toxicity drivers prioritizing associated risks. This review evaluates analytical techniques currently available assess effects, provides structured plan systematic integration into EWS hazardous chemicals environment. Key scientific advancements effect-based approaches EDA are discussed, underscoring potential detection management hazards. Additionally, critical challenges such as data alignment addressed, emphasizing need continuous improvement incorporation safeguard environmental public health from threats.

Язык: Английский

Процитировано

4