Proteomics: An In‐Depth Review on Recent Technical Advances and Their Applications in Biomedicine

Medicinal Research Reviews, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

ABSTRACT Proteins hold pivotal importance since many diseases manifest changes in protein activity. Proteomics techniques provide a comprehensive exploration of structure, abundance, and function biological samples, enabling the holistic characterization overall organisms. Nowadays, breadth emerging methodologies proteomics is unprecedentedly vast, with constant optimization technologies sample processing, data collection, analysis, its scope application steadily transitioning from bench to clinic. Here, we offer an insightful review technical developments applications biomedicine over past 5 years. We focus on profound contributions profiling disease spectra, discovering new biomarkers, identifying promising drug targets, deciphering alterations conformation, unearthing protein–protein interactions. Moreover, summarize cutting‐edge potential breakthroughs pipeline principal challenges proteomics. Based these, aspire broaden applicability inspire researchers enhance our understanding complex systems by utilizing such techniques.

Язык: Английский

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Identification of new endocrine disruptive transthyretin ligands in polluted waters using pull-down assay coupled to non-target mass spectrometry

Journal of Hazardous Materials, Год журнала: 2024, Номер 471, С. 134240 - 134240

Опубликована: Апрель 10, 2024

Язык: Английский

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Multi-omics Integration Analysis: Tools and Applications in Environmental Toxicology

Environmental Pollution, Год журнала: 2024, Номер 360, С. 124675 - 124675

Опубликована: Авг. 3, 2024

Язык: Английский

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Amino Acid Transporter as a Potential Carrier Protein for the Root-to-Shoot Translocation of Polybrominated Diphenyl Ethers in Rice

Environmental Science & Technology, Год журнала: 2023, Номер 57(26), С. 9722 - 9731

Опубликована: Июнь 23, 2023

As typical persistent organic pollutants, polybrominated diphenyl ethers (PBDEs) tend to accumulate in edible parts of rice, posing great ecological and health risks. The translocation PBDEs from underground aboveground rice is a crucial procedure determine the final bioaccumulation level. Herein, this study aimed identify transporter proteins for plants order strengthen our understanding mechanism potential prevention strategy PBDE risk. Similar time-dependent patterns were observed among root-to-shoot factors (TFs) PBDEs, expression lysine histidine (LHT) protein, relative levels LHT substrates (phenylalanine or tyrosine), implying co-transport phenylalanine, tyrosine by carrier LHT. Fluorescence spectra circular dichroism showed that congeners interfered with via static fluorescence quenching changes protein's secondary structure. vitro sorption fraction as revealed equilibrium analysis, was comparable vivo TF values. Knockout OsLHT1 using CRISPR/Cas9 technology caused 48.2–78.4% decrease translocation. Molecular docking simulation suggested inserted into same ligand-binding cavity LHT, substantiating role conformational perspective. Quantitative structure activity relationship analysis demonstrated ether-bond oxygen carbons at site 4 4′ molecules are significant determinants binding affinity protein PBDEs. In summary, discovered acts cellular offered comprehensive molecular explanation plants, covering both biological chemical perspectives. These findings fill knowledge gap on endogenous exogenous pollutants.

Язык: Английский

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A brief review on the assessment of potential joint effects of complex mixtures of contaminants in the environment

Environmental Science Advances, Год журнала: 2024, Номер 3(5), С. 661 - 675

Опубликована: Янв. 1, 2024

Many contaminants can have long-term effects on organisms when they are exposed to low concentrations for extended periods. This review presents new methods identifying the of chemical mixtures.

Язык: Английский

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Quality evaluation parameter and classification model for effluents of wastewater treatment plant based on machine learning

Water Research, Год журнала: 2024, Номер 268, С. 122696 - 122696

Опубликована: Окт. 24, 2024

Язык: Английский

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Interactions between biofilms and PFASs in aquatic ecosystems: Literature exploration

The Science of The Total Environment, Год журнала: 2023, Номер 906, С. 167469 - 167469

Опубликована: Сен. 29, 2023

Язык: Английский

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Disclosing Environmental Ligands of L-FABP and PPARγ: Should We Re-evaluate the Chemical Safety of Hydrocarbon Surfactants?

Environmental Science & Technology, Год журнала: 2023, Номер 57(32), С. 11913 - 11925

Опубликована: Авг. 1, 2023

Chemical contaminants can cause adverse effects by binding to the liver-fatty acid protein (L-FABP) and peroxisome proliferator-activated nuclear receptor γ (PPARγ), which are vital in lipid metabolism. However, presence of numerous compounds environment has hindered identification their ligands, thus only a small portion have been discovered date. In this study, Affinity Purification with Nontargeted Analysis (APNA) was employed identify ligands L-FABP PPARγ indoor dust sewage sludge. A total 83 nonredundant features were pulled-out His-tagged as putative among 13 assigned fatty acids hydrocarbon surfactants. contrast, six isolated when LBD used bait. The surfactants confirmed using both recombinant proteins reporter cells. These surfactants, along >50 homologues isomers, detected sludge at high concentrations. Fatty explained majority (57.7 ± 32.9%) (66.0 27.1%) activities This study revealed predominant synthetic PPARγ, highlighting importance re-evaluating chemical safety.

Язык: Английский

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Identification and Prioritization of Organic Pollutants in Human Milk from the Yangtze River Delta, China

Environmental Science & Technology, Год журнала: 2024, Номер 58(27), С. 11935 - 11944

Опубликована: Июнь 24, 2024

Pollutants in human milk are critical for evaluating maternal internal exposure and infant external exposure. However, most studies have focused on a limited range of pollutants. Here, 15 pooled samples (prepared from 467 individual samples) three areas the Yangtze River Delta (YRD) China were analyzed by gas chromatography quadrupole time-of-flight mass spectrometry. In total, 171 compounds nine types preliminarily identified. Among these, 16 compounds, including 2,5-di-tert-butylhydroquinone 2-tert-butyl-1,4-benzoquinone, detected first time. Partial least-squares discriminant analysis identified ten area-specific pollutants, 2-naphthylamine, 9-fluorenone, 2-isopropylthianthrone, benzo[a]pyrene, among Shanghai (n = 3), Jiangsu Province 6), Zhejiang 6). Risk index (RI) values calculated indicated that legacy polycyclic aromatic hydrocarbons (PAHs) contributed only 20% total RIs PAHs derivatives, indicating more attention should be paid to with various functional groups. Nine priority pollutants YRD The important 4-tert-amylphenol, caffeine, 2,6-di-tert-butyl-p-benzoquinone, which associated apoptosis, oxidative stress, other health hazards. results improve our ability assess risks posed milk.

Язык: Английский

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Identification of Hydrocarbon Sulfonates as Previously Overlooked Transthyretin Ligands in the Environment

Environmental Science & Technology, Год журнала: 2024, Номер 58(23), С. 10227 - 10239

Опубликована: Май 31, 2024

Incidences of thyroid disease, which has long been hypothesized to be partially caused by exposure hormone disrupting chemicals (TDCs), have rapidly increased in recent years. However, known TDCs can only explain a small portion (∼1%) vitro human transthyretin (hTTR) binding activities environmental samples, indicating the existence unknown hTTR ligands. In this study, we aimed identify major ligands employing protein Affinity Purification with Nontargeted Analysis (APNA). were detected all 11 indoor dust and 9 out 10 sewage sludge samples FITC-T4 displacement assay. By using APNA, 31 putative including perfluorooctanesulfonate (PFOS). Two most abundant identified as hydrocarbon surfactants (e.g., dodecyl benzenesulfonate). Moreover, another ligand was surprisingly disulfonate fluorescent brightener, 4,4′-bis(2-sulfostyryl)biphenyl sodium (CBS). CBS validated nM-affinity an IC50 345 nM. total, brighteners 1.92–17.0 5.74–54.3% respectively, whereas PFOS contributed <0.0001%. Our study revealed for first time that sulfonates are previously overlooked environment.

Язык: Английский

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