Incidences
of
thyroid
disease,
which
has
long
been
hypothesized
to
be
partially
caused
by
exposure
hormone
disrupting
chemicals
(TDCs),
have
rapidly
increased
in
recent
years.
However,
only
~1%
the
binding
activity
human
transthyretin
(hTTR),
an
important
transporter
protein,
can
explained
known
TDCs.
In
this
study,
we
aimed
identify
major
hTTR
ligands
Canadian
indoor
dust
and
sewage
sludge
employing
protein-guided
nontargeted
analysis.
activities
were
detected
all
11
9
out
10
samples
(median
458
1134
μg
T4/g
sludge,
respectively)
FITC-T4
displacement
assay.
Through
protein
Affinity
Purification
with
Nontargeted
Analysis
(APNA),
31
putative
including
perfluorooctane
sulfonate
(PFOS).
Two
most
abundant
identified
as
hydrocarbon
surfactants
(e.g.,
dodecyl
benzenesulfonate),
confirmed
authentic
chemical
standards.
Structure-activity
relationships
(SAR)
explored
investigating
hTTR.
Optimal
carbon
chain
length
(C12-14)
was
found
achieve
a
high
affinity.
By
de
novo
analysis,
another
ligand
surprisingly
di-sulfonate
fluorescent
brightener,
4,4'-Bis(2-sulfostyryl)biphenyl
sodium
(CBS).
CBS
validated
nM-affinity
IC50
345
nM.
total,
brightener
could
explain
1.92-17.0%
5.74-54.3%
samples,
respectively,
whereas
PFOS
contributed
<0.0001%
activity.
Our
study
revealed
for
first
time
that
sulfonates
are
previously
overlooked
environment.
Medicinal Research Reviews,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 9, 2025
ABSTRACT
Proteins
hold
pivotal
importance
since
many
diseases
manifest
changes
in
protein
activity.
Proteomics
techniques
provide
a
comprehensive
exploration
of
structure,
abundance,
and
function
biological
samples,
enabling
the
holistic
characterization
overall
organisms.
Nowadays,
breadth
emerging
methodologies
proteomics
is
unprecedentedly
vast,
with
constant
optimization
technologies
sample
processing,
data
collection,
analysis,
its
scope
application
steadily
transitioning
from
bench
to
clinic.
Here,
we
offer
an
insightful
review
technical
developments
applications
biomedicine
over
past
5
years.
We
focus
on
profound
contributions
profiling
disease
spectra,
discovering
new
biomarkers,
identifying
promising
drug
targets,
deciphering
alterations
conformation,
unearthing
protein–protein
interactions.
Moreover,
summarize
cutting‐edge
potential
breakthroughs
pipeline
principal
challenges
proteomics.
Based
these,
aspire
broaden
applicability
inspire
researchers
enhance
our
understanding
complex
systems
by
utilizing
such
techniques.
Environmental Science & Technology,
Journal Year:
2023,
Volume and Issue:
57(26), P. 9722 - 9731
Published: June 23, 2023
As
typical
persistent
organic
pollutants,
polybrominated
diphenyl
ethers
(PBDEs)
tend
to
accumulate
in
edible
parts
of
rice,
posing
great
ecological
and
health
risks.
The
translocation
PBDEs
from
underground
aboveground
rice
is
a
crucial
procedure
determine
the
final
bioaccumulation
level.
Herein,
this
study
aimed
identify
transporter
proteins
for
plants
order
strengthen
our
understanding
mechanism
potential
prevention
strategy
PBDE
risk.
Similar
time-dependent
patterns
were
observed
among
root-to-shoot
factors
(TFs)
PBDEs,
expression
lysine
histidine
(LHT)
protein,
relative
levels
LHT
substrates
(phenylalanine
or
tyrosine),
implying
co-transport
phenylalanine,
tyrosine
by
carrier
LHT.
Fluorescence
spectra
circular
dichroism
showed
that
congeners
interfered
with
via
static
fluorescence
quenching
changes
protein's
secondary
structure.
vitro
sorption
fraction
as
revealed
equilibrium
analysis,
was
comparable
vivo
TF
values.
Knockout
OsLHT1
using
CRISPR/Cas9
technology
caused
48.2–78.4%
decrease
translocation.
Molecular
docking
simulation
suggested
inserted
into
same
ligand-binding
cavity
LHT,
substantiating
role
conformational
perspective.
Quantitative
structure
activity
relationship
analysis
demonstrated
ether-bond
oxygen
carbons
at
site
4
4′
molecules
are
significant
determinants
binding
affinity
protein
PBDEs.
In
summary,
discovered
acts
cellular
offered
comprehensive
molecular
explanation
plants,
covering
both
biological
chemical
perspectives.
These
findings
fill
knowledge
gap
on
endogenous
exogenous
pollutants.
Environmental Science Advances,
Journal Year:
2024,
Volume and Issue:
3(5), P. 661 - 675
Published: Jan. 1, 2024
Many
contaminants
can
have
long-term
effects
on
organisms
when
they
are
exposed
to
low
concentrations
for
extended
periods.
This
review
presents
new
methods
identifying
the
of
chemical
mixtures.
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(27), P. 11935 - 11944
Published: June 24, 2024
Pollutants
in
human
milk
are
critical
for
evaluating
maternal
internal
exposure
and
infant
external
exposure.
However,
most
studies
have
focused
on
a
limited
range
of
pollutants.
Here,
15
pooled
samples
(prepared
from
467
individual
samples)
three
areas
the
Yangtze
River
Delta
(YRD)
China
were
analyzed
by
gas
chromatography
quadrupole
time-of-flight
mass
spectrometry.
In
total,
171
compounds
nine
types
preliminarily
identified.
Among
these,
16
compounds,
including
2,5-di-tert-butylhydroquinone
2-tert-butyl-1,4-benzoquinone,
detected
first
time.
Partial
least-squares
discriminant
analysis
identified
ten
area-specific
pollutants,
2-naphthylamine,
9-fluorenone,
2-isopropylthianthrone,
benzo[a]pyrene,
among
Shanghai
(n
=
3),
Jiangsu
Province
6),
Zhejiang
6).
Risk
index
(RI)
values
calculated
indicated
that
legacy
polycyclic
aromatic
hydrocarbons
(PAHs)
contributed
only
20%
total
RIs
PAHs
derivatives,
indicating
more
attention
should
be
paid
to
with
various
functional
groups.
Nine
priority
pollutants
YRD
The
important
4-tert-amylphenol,
caffeine,
2,6-di-tert-butyl-p-benzoquinone,
which
associated
apoptosis,
oxidative
stress,
other
health
hazards.
results
improve
our
ability
assess
risks
posed
milk.
Metabolism-disrupting
chemicals
(MDCs)
have
attracted
widespread
attention
due
to
their
contributions
the
prevalence
of
metabolic
diseases
worldwide.
The
farnesoid
X
receptor
(FXR)
is
a
typical
lipid-sensing
nuclear
and
plays
crucial
role
in
development
diseases.
However,
few
studies
examined
FXR
activities
environmental
samples
corresponding
MDCs.
In
this
study,
we
found
FXR-antagonistic
93.6%
source
water,
surface
wastewater
(n
=
78)
collected
from
Yangtze
River
Yellow
River.
An
protein-affinity
guided
nontargeted
analysis
was
performed
identified
79
potential
FXR-active
pollutants
these
two
rivers.
Nine
exhibited
strong
(IC50:
2.39–141.9
μM),
6
pollutants,
including
triphenyl
phosphate
(TPHP),
4,4′-sulfonylbis[2-(2-propenyl)
phenol
(TGSA),
tonalid
(AHTN),
dichlorophen,
etoxazole
(ETX),
loratadine,
were
be
antagonists
for
first
time.
total
concentrations
nine
relatively
high
middle
downstream
reaches
River,
(TGSA
ETX)
not
previously
been
aquatic
environments.
A
risk
prioritization
revealed
that
TPHP,
TGSA,
AHTN
are
priority
with
affect
FXR.
Appropriate
management
would
reduce
health
risks
disruptions
associated
exposure
