Paeoniflorin mitigates high glucose-induced lifespan reduction by inhibiting insulin signaling in Caenorhabditis elegans

Frontiers in Pharmacology, Год журнала: 2023, Номер 14

Опубликована: Июнь 19, 2023

In organisms, high glucose can cause several aspects of toxicity, including the lifespan reduction. Paeoniflorin is major component Paeoniaceae plants. Nevertheless, possible effect paeoniflorin to suppress toxicity in reducing and underlying mechanism are largely unclear. Thus, this study, we examined suppressing (50 mM)-induced reduction Caenorhabditis elegans . Administration with 16–64 mg/L could prolong treated nematodes. Accompanied beneficial effect, nematodes, expressions daf-2 encoding insulin receptor its downstream kinase genes ( age-1 , akt-1 akt-2 ) were decreased expression daf-16 FOXO transcriptional factor was increased by administration. Meanwhile, extending nematodes enhanced RNAi inhibited followed administration, caused be suppressed suggesting that DAF-2 acted upstream DAF-16 regulate pharmacological paeoniflorin. Moreover, sod-3 mitochondrial Mn-SOD RNAi, RNAi. Molecular docking analysis indicated binding potential DAF-2, AGE-1, AKT-1, AKT-2. Therefore, our results demonstrated administration inhibiting glucose-induced signaling cascade DAF-2-AGE-1-AKT-1/2-DAF-16-SOD-3 pathway.

Язык: Английский

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Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of Caenorhabditis elegans

Toxics, Год журнала: 2023, Номер 11(6), С. 511 - 511

Опубликована: Июнь 6, 2023

Recently, the transgenerational toxicity of nanoplastics has received increasing attention. Caenorhabditis elegans is a useful model to assess different pollutants. In nematodes, possibility early-life exposure sulfonate-modified polystyrene nanoparticle (PS-S NP) causing and its underlying mechanisms were investigated. After at L1-larval stage, inhibition in both locomotion behavior (body bend head thrash) reproductive capacity (number offspring fertilized egg number uterus) was induced by 1-100 μg/L PS-S NP. Meanwhile, after NP, expression germline lag-2 encoding Notch ligand increased not only parental generation (P0-G) but also offspring, inhibited RNA interference (RNAi) lag-2. During formation, LAG-2 activated corresponding receptor GLP-1 suppressed glp-1 RNAi. functioned neurons mediate NP toxicity. NP-exposed insulin peptides INS-39, INS-3, DAF-28, neuronal DAF-7, DBL-1, GLB-10. Therefore, risk inducing through suggested, this mediated activation signal organisms.

Язык: Английский

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Polylactic acid microparticles in the range of μg/L reduce reproductive capacity by affecting the gonad development and the germline apoptosis in Caenorhabditis elegans

Chemosphere, Год журнала: 2023, Номер 336, С. 139193 - 139193

Опубликована: Июнь 12, 2023

Язык: Английский

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Stable isotope-assisted mass spectrometry reveals in vivo distribution, metabolism, and excretion of tire rubber-derived 6PPD-quinone in mice

The Science of The Total Environment, Год журнала: 2023, Номер 912, С. 169291 - 169291

Опубликована: Дек. 15, 2023

Язык: Английский

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Paeoniflorin increases the survival of Pseudomonas aeruginosa infected Caenorhabditis elegans at the immunosuppression stage by activating PMK-1, BAR-1, and EGL-1 signals

Archives of Pharmacal Research, Год журнала: 2023, Номер 46(7), С. 616 - 628

Опубликована: Июль 1, 2023

Язык: Английский

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