ACS ES&T Engineering, Год журнала: 2024, Номер unknown

Опубликована: Окт. 8, 2024

Thiosulfate-assisted anaerobic fermentation (AF) effectively converts waste activated sludge into high-value products (e.g., short-chain fatty acids (SCFAs)). However, the roles of thiosulfate in organics transformation, electron transfer, and microbial interactions within AF systems are not fully understood, especially under long-term operations. In this study, an 88 day experiment was conducted to address knowledge gap. The results indicated average SCFA yield 3625.1 mg COD/L acetate proportion 49.4% with a dosage 600 S/L. Model organic degradation tests revealed that functioned as acceptor, facilitating NAD+/NADH stimulating expression protein complexes like cytochrome c enhance transport, lowering thermodynamic barriers propionate butyrate (ΔG1propionate = −335.0 kJ/mol; ΔG2butyrate −113.8 kJ/mol). Molecular ecological networks analysis showed strengthened cooperative relationships among biomarkers hydrolytic bacteria (i.e., Proteiniphilum, UBA5851), acidogenic UBA4179), sulfur reducers JAEUSI01). Functional gene using random forest confirmed upregulated key genes 2-oxoacid ferredoxin oxidoreductase) associated transfer metabolism. This study deepens our understanding thiosulfate, strengthening cooperation systems.

Язык: Английский