Ratiometric Optical Sensing of Aerosol Phase State with Excited-State Intramolecular Proton Transfer Probes DOI

Angel M. Gibbons,

Paul E. Ohno

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Июнь 5, 2025

Phase transitions in respiratory and environmental aerosols impact critical processes ranging from virus transmission to atmospheric light scattering. Yet, small particle sizes low mass densities air make experimental measurements of aerosol phase state challenging. Fluorescence probe spectroscopy is one the only analytical techniques capable determining situ at submicron that are implicated long-range dominate size distribution atmosphere. However, previous fluorescent probe-based have relied on solvatochromic molecules their associated relatively shifts emission wavelength, necessitating high-resolution spectral greatly limiting optical throughput therefore sensitivity. Here, demonstrated using a different class molecules, excited-state intramolecular proton transfer (ESIPT) probes, exhibit two peaks with an intensity ratio highly dependent surrounding chemical environment. The ESIPT 2-(2-benzofuranyl)-3-hydroxychromone shown be sensitive state, including both solid-liquid liquid-liquid transitions, mixed organic/inorganic aerosols. origin sensitivity was investigated by varying identity constituents results indicate particularly presence Na+ Cl- ions, which involved key particles as well sea-spray These findings highlight potential ESIPT-based sensing powerful technique for real time analysis combining unprecedented simplicity.

Язык: Английский

Mucin Colocalizes with Influenza Virus and Preserves Infectivity in Deposited Model Respiratory Droplets DOI
Jin Pan, Nisha K. Duggal, Seema S. Lakdawala

и другие.

Environmental Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 17, 2025

The stability of influenza virus in respiratory particles varies with relative humidity (RH) and protein content. This study investigated the decay, or loss infectivity, A (IAV) 1-μL droplets deposited on a surface varying concentrations mucin, one most abundant proteins mucus, examined localization virions within droplets. IAV remained stable at 0.1% 0.5% mucin phosphate-buffered saline (PBS) over 4 h 20%, 50%, 80% RH, maximum decay 1.2 log10/mL. In contrast, pure PBS droplets, decayed by least 2.6 log10/mL after 50% RH. Mucin's protective effect was independent its concentration, except RH h. Confocal microscopy revealed that 20% led to thicker coffee rings dendritic patterns where colocalized mucin. At no morphological difference observed between PBS-only mucin-containing but still center Analysis digital droplet PCR showed helped maintain integrity. To our knowledge, this is first localize model results suggest mucin's colocalization may protect from environmental stressors, enhancing stability.

Язык: Английский

Процитировано

0

Assessment of flavivirus RNA stability and infectivity in various water environments DOI Creative Commons

Yabunji SANO,

Hawraa Al-Alawi,

Misao Himeno

и другие.

Tropical Medicine and Health, Год журнала: 2025, Номер 53(1)

Опубликована: Янв. 24, 2025

Abstract Introduction Flaviviruses such as dengue virus (DENV), Zika (ZIKV), Japanese encephalitis (JEV), and Yellow fever (YFV) are mosquito-borne RNA viruses causing major public health threats in parts of the world. While DENV ZIKV have been detected urine samples, data on presence stability flaviviruses water environment limited. Methods In this study, we determined infectivity flavivirus different environments by utilizing RT-qPCR plaque assay to explore feasibility environmental detection surveillance flaviviruses. Results Viral could be for up 49-days, at 4 °C, 25 °C 37 temperatures, infectious particles 7 days. our findings showed that flaviviral has higher better rates lower capacity was comparatively short Conclusions Our results indicate retains limited general highlight various epidemiologic settings.

Язык: Английский

Процитировано

0

Ratiometric Optical Sensing of Aerosol Phase State with Excited-State Intramolecular Proton Transfer Probes DOI

Angel M. Gibbons,

Paul E. Ohno

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Июнь 5, 2025

Phase transitions in respiratory and environmental aerosols impact critical processes ranging from virus transmission to atmospheric light scattering. Yet, small particle sizes low mass densities air make experimental measurements of aerosol phase state challenging. Fluorescence probe spectroscopy is one the only analytical techniques capable determining situ at submicron that are implicated long-range dominate size distribution atmosphere. However, previous fluorescent probe-based have relied on solvatochromic molecules their associated relatively shifts emission wavelength, necessitating high-resolution spectral greatly limiting optical throughput therefore sensitivity. Here, demonstrated using a different class molecules, excited-state intramolecular proton transfer (ESIPT) probes, exhibit two peaks with an intensity ratio highly dependent surrounding chemical environment. The ESIPT 2-(2-benzofuranyl)-3-hydroxychromone shown be sensitive state, including both solid-liquid liquid-liquid transitions, mixed organic/inorganic aerosols. origin sensitivity was investigated by varying identity constituents results indicate particularly presence Na+ Cl- ions, which involved key particles as well sea-spray These findings highlight potential ESIPT-based sensing powerful technique for real time analysis combining unprecedented simplicity.

Язык: Английский

Процитировано

0