Mucin Colocalizes with Influenza Virus and Preserves Infectivity in Deposited Model Respiratory Droplets
Environmental Science & Technology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 17, 2025
The
stability
of
influenza
virus
in
respiratory
particles
varies
with
relative
humidity
(RH)
and
protein
content.
This
study
investigated
the
decay,
or
loss
infectivity,
A
(IAV)
1-μL
droplets
deposited
on
a
surface
varying
concentrations
mucin,
one
most
abundant
proteins
mucus,
examined
localization
virions
within
droplets.
IAV
remained
stable
at
0.1%
0.5%
mucin
phosphate-buffered
saline
(PBS)
over
4
h
20%,
50%,
80%
RH,
maximum
decay
1.2
log10/mL.
In
contrast,
pure
PBS
droplets,
decayed
by
least
2.6
log10/mL
after
50%
RH.
Mucin's
protective
effect
was
independent
its
concentration,
except
RH
h.
Confocal
microscopy
revealed
that
20%
led
to
thicker
coffee
rings
dendritic
patterns
where
colocalized
mucin.
At
no
morphological
difference
observed
between
PBS-only
mucin-containing
but
still
center
Analysis
digital
droplet
PCR
showed
helped
maintain
integrity.
To
our
knowledge,
this
is
first
localize
model
results
suggest
mucin's
colocalization
may
protect
from
environmental
stressors,
enhancing
stability.
Язык: Английский
Assessment of flavivirus RNA stability and infectivity in various water environments
Tropical Medicine and Health,
Год журнала:
2025,
Номер
53(1)
Опубликована: Янв. 24, 2025
Abstract
Introduction
Flaviviruses
such
as
dengue
virus
(DENV),
Zika
(ZIKV),
Japanese
encephalitis
(JEV),
and
Yellow
fever
(YFV)
are
mosquito-borne
RNA
viruses
causing
major
public
health
threats
in
parts
of
the
world.
While
DENV
ZIKV
have
been
detected
urine
samples,
data
on
presence
stability
flaviviruses
water
environment
limited.
Methods
In
this
study,
we
determined
infectivity
flavivirus
different
environments
by
utilizing
RT-qPCR
plaque
assay
to
explore
feasibility
environmental
detection
surveillance
flaviviruses.
Results
Viral
could
be
for
up
49-days,
at
4
°C,
25
°C
37
temperatures,
infectious
particles
7
days.
our
findings
showed
that
flaviviral
has
higher
better
rates
lower
capacity
was
comparatively
short
Conclusions
Our
results
indicate
retains
limited
general
highlight
various
epidemiologic
settings.
Язык: Английский
Ratiometric Optical Sensing of Aerosol Phase State with Excited-State Intramolecular Proton Transfer Probes
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Июнь 5, 2025
Phase
transitions
in
respiratory
and
environmental
aerosols
impact
critical
processes
ranging
from
virus
transmission
to
atmospheric
light
scattering.
Yet,
small
particle
sizes
low
mass
densities
air
make
experimental
measurements
of
aerosol
phase
state
challenging.
Fluorescence
probe
spectroscopy
is
one
the
only
analytical
techniques
capable
determining
situ
at
submicron
that
are
implicated
long-range
dominate
size
distribution
atmosphere.
However,
previous
fluorescent
probe-based
have
relied
on
solvatochromic
molecules
their
associated
relatively
shifts
emission
wavelength,
necessitating
high-resolution
spectral
greatly
limiting
optical
throughput
therefore
sensitivity.
Here,
demonstrated
using
a
different
class
molecules,
excited-state
intramolecular
proton
transfer
(ESIPT)
probes,
exhibit
two
peaks
with
an
intensity
ratio
highly
dependent
surrounding
chemical
environment.
The
ESIPT
2-(2-benzofuranyl)-3-hydroxychromone
shown
be
sensitive
state,
including
both
solid-liquid
liquid-liquid
transitions,
mixed
organic/inorganic
aerosols.
origin
sensitivity
was
investigated
by
varying
identity
constituents
results
indicate
particularly
presence
Na+
Cl-
ions,
which
involved
key
particles
as
well
sea-spray
These
findings
highlight
potential
ESIPT-based
sensing
powerful
technique
for
real
time
analysis
combining
unprecedented
simplicity.
Язык: Английский