Assessment of flavivirus RNA stability and infectivity in various water environments DOI Creative Commons

Yabunji SANO,

Hawraa Al-Alawi,

Misao Himeno

et al.

Tropical Medicine and Health, Journal Year: 2025, Volume and Issue: 53(1)

Published: Jan. 24, 2025

Abstract Introduction Flaviviruses such as dengue virus (DENV), Zika (ZIKV), Japanese encephalitis (JEV), and Yellow fever (YFV) are mosquito-borne RNA viruses causing major public health threats in parts of the world. While DENV ZIKV have been detected urine samples, data on presence stability flaviviruses water environment limited. Methods In this study, we determined infectivity flavivirus different environments by utilizing RT-qPCR plaque assay to explore feasibility environmental detection surveillance flaviviruses. Results Viral could be for up 49-days, at 4 °C, 25 °C 37 temperatures, infectious particles 7 days. our findings showed that flaviviral has higher better rates lower capacity was comparatively short Conclusions Our results indicate retains limited general highlight various epidemiologic settings.

Language: Английский

Mucin Colocalizes with Influenza Virus and Preserves Infectivity in Deposited Model Respiratory Droplets DOI
Jin Pan, Nisha K. Duggal, Seema S. Lakdawala

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

The stability of influenza virus in respiratory particles varies with relative humidity (RH) and protein content. This study investigated the decay, or loss infectivity, A (IAV) 1-μL droplets deposited on a surface varying concentrations mucin, one most abundant proteins mucus, examined localization virions within droplets. IAV remained stable at 0.1% 0.5% mucin phosphate-buffered saline (PBS) over 4 h 20%, 50%, 80% RH, maximum decay 1.2 log10/mL. In contrast, pure PBS droplets, decayed by least 2.6 log10/mL after 50% RH. Mucin's protective effect was independent its concentration, except RH h. Confocal microscopy revealed that 20% led to thicker coffee rings dendritic patterns where colocalized mucin. At no morphological difference observed between PBS-only mucin-containing but still center Analysis digital droplet PCR showed helped maintain integrity. To our knowledge, this is first localize model results suggest mucin's colocalization may protect from environmental stressors, enhancing stability.

Language: Английский

Citations

0
Assessment of flavivirus RNA stability and infectivity in various water environments DOI Creative Commons

Yabunji SANO,

Hawraa Al-Alawi,

Misao Himeno

et al.

Tropical Medicine and Health, Journal Year: 2025, Volume and Issue: 53(1)

Published: Jan. 24, 2025

Abstract Introduction Flaviviruses such as dengue virus (DENV), Zika (ZIKV), Japanese encephalitis (JEV), and Yellow fever (YFV) are mosquito-borne RNA viruses causing major public health threats in parts of the world. While DENV ZIKV have been detected urine samples, data on presence stability flaviviruses water environment limited. Methods In this study, we determined infectivity flavivirus different environments by utilizing RT-qPCR plaque assay to explore feasibility environmental detection surveillance flaviviruses. Results Viral could be for up 49-days, at 4 °C, 25 °C 37 temperatures, infectious particles 7 days. our findings showed that flaviviral has higher better rates lower capacity was comparatively short Conclusions Our results indicate retains limited general highlight various epidemiologic settings.

Language: Английский

Citations

0