Biological Effects of Micro-/Nano-Plastics in Macrophages
Nanomaterials,
Год журнала:
2025,
Номер
15(5), С. 394 - 394
Опубликована: Март 4, 2025
The
environmental
impact
of
plastics
is
worsened
by
their
inadequate
end-of-life
disposal,
leading
to
the
ubiquitous
presence
micro-
(MPs)
and
nanosized
(NPs)
plastic
particles.
MPs
NPs
are
thus
widely
present
in
water
air
inevitably
enter
food
chain,
with
inhalation
ingestion
as
main
exposure
routes
for
humans.
Many
recent
studies
have
demonstrated
that
gain
access
several
body
compartments,
where
they
taken
up
cells,
increase
production
reactive
oxygen
species,
lead
inflammatory
changes.
In
most
tissues,
resident
macrophages
engage
first
approach
foreign
materials,
this
interaction
largely
affects
subsequent
fate
material
possible
pathological
outcomes.
On
other
hand,
organizers
controllers
both
responses
tissue
repair.
Here,
we
aim
summarize
available
information
on
NPs.
Particular
attention
will
be
devoted
consequences
macrophage
viability
functions,
well
implications
pathology.
Язык: Английский
Predicting Protein Corona Formation on Polylactic Acid Microplastics Pre- and Post-Photoaging: The Importance of Optimal Imputation Methods
Environmental Science & Technology Letters,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 4, 2025
Micro-nanoplastics
(MNPs)
enter
biological
systems,
forming
a
protein
corona
(PC)
by
adsorbing
proteins
from
bodily
fluids,
influencing
their
effects.
Mass
spectrometry-based
proteomics
characterizes
PC
composition,
and
recent
advances
have
leveraged
amino
acid
sequence-derived
features
to
predict
formation
using
supervised
random
forest
(RF)
classifier.
However,
mass
spectrometry
often
generates
substantial
missing
values
(MVs),
which
may
hinder
the
model's
predictive
performance
understanding
of
protein–particle
interactions.
This
study
assessed
impact
20
imputation
methods
on
RF
classifier
in
predicting
human
plasma
polylactic
(PLA)
photoaged
PLA
microplastics
(MPs),
considering
rising
ecological
health
concerns.
The
results
showed
that
five
left-censored
(Zero,
Half-min,
Min,
QRILC,
GSimp)
achieved
best
performance,
with
high
accuracy
(0.80–0.82),
AUC
(0.78–0.84),
precision
(0.78–0.80),
recall
(0.97–0.98).
Protein
spatial
features,
including
secondary
sheet
structure
(negative)
absolute
solvent-accessible
area
(positive),
were
identified
as
key
factors
adsorption
onto
MPs.
Additionally,
UV
aging
increased
importance
ranking
frac_aa_S
fraction_exposed_exposed_S,
highlighting
altered
protein–MPs
interactions,
likely
through
hydrogen
bonding
electrostatic
forces.
demonstrates
potential
enhancing
for
formation.
Язык: Английский
A Review of Eco-Corona Formation on Micro/Nanoplastics and Its Effects on Stability, Bioavailability, and Toxicity
Water,
Год журнала:
2025,
Номер
17(8), С. 1124 - 1124
Опубликована: Апрель 10, 2025
Micro/nanoplastics
(M/NPs)
have
become
prevalent
in
aquatic
environments
due
to
their
widespread
applications.
Likewise,
ubiquitous
ecological
macromolecules
can
adsorb
onto
M/NPs
form
an
“eco-corona”,
which
significantly
alters
environmental
behaviors
including
aggregation
dynamics,
adsorption/desorption,
and
bioavailability.
Therefore,
it
is
necessary
analyze
the
role
of
eco-corona
assessing
risks
M/NPs.
This
review
systematically
summarizes
formation
mechanisms
evaluates
its
regulatory
effects
on
stability
ecotoxicity
Compared
with
other
(e.g.,
natural
organic
matter
extracellular
polymeric
substances),
humic
acid
(HA)
tightly
binds
through
electrostatic
hydrophobic
interactions,
affecting
hetero-aggregation
behavior
colloidal
stability.
In
terms
bioavailability,
various
functional
groups
HA
surface
regulate
charge
hydrophobicity
M/NPs,
thereby
bioaccumulation
“Trojan
horse”
effect.
Notably,
corona
alleviates
M/NPs-induced
growth
inhibition
oxidative
stress.
Genotoxicity
assessment
further
showed
that
expression
genes
related
stress
response
detoxification
pathways.
Future
studies
should
focus
synergistic
between
co-existing
pollutants
complex
elucidate
long-term
associated
formation.
Язык: Английский
Essential protocols for decoding the composition and the functional effects of the nanoparticle protein corona
Frontiers in Nanotechnology,
Год журнала:
2024,
Номер
6
Опубликована: Окт. 23, 2024
Identifying
the
function
and
composition
of
protein
corona
(i.e.,
set
host
proteins
interacting
with
nanoparticles)
is
considered
a
crucial
step
in
development
nanoparticles
for
medical
pharmacological
applications.
Evidence
suggests
that
can
alter
NP
stability,
biocompatibility,
pharmacokinetics
features.
Therefore,
this
review,
we
provide
an
updated
conceptual,
methodological,
experimental
guideline
study
corona.
We
surveyed
recent
literature
(2009–2024)
focusing
on
vitro
vivo
studies.
show
several
methods,
including
shot-gun
proteomics,
identification
after
in-gel
digestion,
TMT
must
be
carefully
applied
integrated
to
shed
light
complex
phenomenon.
Hence,
discuss
detail
relative
protocols,
highlighting
importance
conditions,
ranging
from
administration
route
basic,
but
determinant,
parameters
like
kind
biological
fluids,
incubation
times
concentrations.
Additionally,
propose
series
protocols
involve
studying
using
purified
serum
or
plasma
proteins,
as
well
sera
depleted
specific
complement
investigate
role
their
deposition
nanoparticle
surface.
also
explore
how
inducing
uptake
by
phagocytic
cells
examined;
finally,
methodological
approaches
effects
different
coatings
Available
data
indicated
it
possible
characterize
punctually
differential
adsorption
onto
This
allows
designing
chemical
features
actively
guide
formation,
thus
improving
nanotheranostic
development.
Язык: Английский