Cell Host & Microbe, Год журнала: 2023, Номер 31(10), С. 1732 - 1747.e5
Опубликована: Сен. 22, 2023
Pathogen infection is a dynamic process. Here, we employ single-cell transcriptomics to investigate plant response heterogeneity. By generating an Arabidopsis thaliana leaf atlas encompassing 95,040 cells during by fungal pathogen, Colletotrichum higginsianum, unveil cell-type-specific gene expression, notably enrichment of intracellular immune receptors in vasculature cells. Trajectory inference identifies that had different interactions with the invading fungus. This analysis divulges transcriptional reprogramming abscisic acid signaling specifically occurring guard cells, which consistent stomatal closure dependent on direct contact Furthermore, plasticity genes involved glucosinolate biosynthesis at sites, emphasizing contribution epidermis-expressed MYB122 disease resistance. work underscores spatially dynamic, responses pathogen and provides valuable resource supports in-depth investigations plant-pathogen interactions.
Язык: Английский
Процитировано
42Light regulation of the biosynthesis of phenolics, terpenoids, and alkaloids in plants
Communications Biology, Год журнала: 2023, Номер 6(1)
Опубликована: Окт. 18, 2023
Abstract Biosynthesis of specialized metabolites (SM), including phenolics, terpenoids, and alkaloids, is stimulated by many environmental factors light. In recent years, significant progress has been made in understanding the regulatory mechanisms involved light-stimulated SM biosynthesis at transcriptional, posttranscriptional, posttranslational levels regulation. While several excellent reviews have primarily focused on impacts general factors, light, an individual class SM, here we highlight regulation three major pathways light-responsive gene expression, microRNA regulation, modification proteins. addition, present our future perspectives this topic.
Язык: Английский
Процитировано
23Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways
Plant Communications, Год журнала: 2023, Номер 4(4), С. 100565 - 100565
Опубликована: Фев. 23, 2023
Glucosinolates (GSLs), found mainly in species of the Brassicaceae family, are one most well-studied classes secondary metabolites. Produced by action myrosinase on GSLs, GSL-derived hydrolysis products (GHPs) primarily defend against biotic stress planta. They also significantly affect quality crop products, with a subset GHPs contributing unique food flavors and multiple therapeutic benefits or causing disagreeable odors health risks. Here, we explore potential these bioactive functions, which could be exploited for future sustainable agriculture. We first summarize our accumulated understanding GSL diversity distribution across representative species. then systematically discuss evaluate unutilized genes involved biosynthesis, transport, as candidate engineering targets. Benefiting from available information GHP options multifunctional ideotypes to meet demand diversification production. An integrated roadmap is subsequently proposed guide ideotype development, maximization beneficial effects minimization detrimental combined associated various end uses. Based several use-case examples, advantages limitations biotechnological approaches that may contribute effective deployment provide novel insights optimization engineering.
Язык: Английский
Процитировано
22Bioinformatics and Functional Genomics
Springer eBooks, Год журнала: 2024, Номер unknown, С. 243 - 271
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
12The role of abiotic and biotic factors of aged brine in directing microbial assembly and volatile profiles of Paocai during fermentation
Food Bioscience, Год журнала: 2024, Номер 57, С. 103592 - 103592
Опубликована: Янв. 8, 2024
Язык: Английский
Процитировано
10Differential Effects of Low Light Intensity on Broccoli Microgreens Growth and Phytochemicals
Agronomy, Год журнала: 2021, Номер 11(3), С. 537 - 537
Опубликована: Март 12, 2021
To produce high-quality broccoli microgreens, suitable light intensity for growth and phytochemical contents of microgreens in an artificial plant factory were studied. Broccoli irradiated under different photosynthetic photon flux density (PPFD): 30, 50, 70 90 μmol·m−2·s−1 with red: green: blue = 1:1:1 light-emitting diodes (LEDs). The grown 50 had the highest fresh weight, dry moisture content, while lowest. With increasing intensity, chlorophyll content increased, whereas carotenoid decreased. soluble protein, sugar, free amino acid, flavonoid, vitamin C, glucosinolates except progoitrin higher μmol·m−2·s−1. Overall, was optimal enhancement more feasible improving phytochemicals factory.
Язык: Английский
Процитировано
51Integrated view of plant metabolic defense with particular focus on chewing herbivores
Journal of Integrative Plant Biology, Год журнала: 2021, Номер 64(2), С. 449 - 475
Опубликована: Дек. 16, 2021
Success of plants largely depends on their ability to defend against herbivores. Since emergence the first voracious consumers, maintained adapting structures and chemistry escape from extinction. The constant pressure was further accelerated by adaptation herbivores plant defenses, which all together sparked rise a chemical empire comprised thousands specialized metabolites currently found in plants. Metabolic diversity kingdom is truly amazing, although many have already been identified, large number potentially useful chemicals remain unexplored bio-resources. Similarly, biosynthetic routes for involve enzymes, some still wait identification biochemical characterization. Moreover, regulatory mechanisms that control gene expression enzyme activities metabolism are scarcely known. Finally, understanding how defense exert toxicity and/or repellency remains limited typical examples, such as proteinase inhibitors, cyanogenic compounds nicotine. In this review, we attempt summarizing current status quo metabolic predominantly based survey ubiquitous examples interactions with chewing
Язык: Английский
Процитировано
41Interactions of fungi with non-isothiocyanate products of the plant glucosinolate pathway: A review on product formation, antifungal activity, mode of action and biotransformation
Phytochemistry, Год журнала: 2022, Номер 200, С. 113245 - 113245
Опубликована: Май 24, 2022
The glucosinolate pathway, which is present in the order Brassicales, one of most researched defensive natural product biosynthesis pathways. Its core molecules, glucosinolates are broken down upon pathogen challenge or tissue damage to yield an array products that may help plants defend against stressor. Though widely known decomposition antimicrobial isothiocyanates, there a wide range other volatile and non-volatile arise from this biosynthetic pathway. This review summarizes our current knowledge on interaction these much less examined, non-isothiocyanate with fungi. It deals compounds including (1) their precursors; (2) glucosinolate-derived nitriles (e.g. derivatives 1H-indole-3-acetonitrile), thiocyanates, epithionitriles oxazolidine-2-thiones; (3) putative isothiocyanate downstream such as raphanusamic acid, 1H-indole-3-methanol (= indole-3-carbinol) its oligomers, 1H-indol-3-ylmethanamine ascorbigen; (4) 1H-indole-3-acetonitrile 1H-indole-3-carbaldehyde (indole-3-carboxaldehyde), 1H-indole-3-carboxylic acid derivatives; (5) indole phytoalexins brassinin, cyclobrassinin brassilexin. Herein, literature following aspects provided: direct antifungal activity proposed mechanisms action, increased after fungal challenge, well data biotransformation/detoxification by fungi, but not limited myrosinase activity.
Язык: Английский
Процитировано
29Specialized metabolite modifications in Brassicaceae seeds and plants: diversity, functions and related enzymes
Natural Product Reports, Год журнала: 2024, Номер 41(5), С. 834 - 859
Опубликована: Янв. 1, 2024
Diversity, functions and related enzymes of Specialized Metabolite (SM) modifications from plants seeds Brassicaceae are summarized in this review. A particular focus is made on SM plasticity modification seeds.
Язык: Английский
Процитировано
7Genome-wide association mapping within a local Arabidopsis thaliana population more fully reveals the genetic architecture for defensive metabolite diversity
Philosophical Transactions of the Royal Society B Biological Sciences, Год журнала: 2022, Номер 377(1855)
Опубликована: Май 30, 2022
A paradoxical finding from genome-wide association studies (GWAS) in plants is that variation metabolite profiles typically maps to a small number of loci, despite the complexity underlying biosynthetic pathways. This discrepancy may partially arise limitations presented by geographically diverse mapping panels. Properties metabolic pathways impede GWAS diluting additive effect causal variant, such as allelic and genetic heterogeneity epistasis, would be expected increase severity with geographical range panel. We hypothesized population single locality reveal an expanded set associated loci. tested this French Arabidopsis thaliana (less than 1 km transect) profiling conducting for glucosinolates, suite defensive metabolites have been studied depth through functional approaches. For two distinct classes we discovered more associations at loci previous continental-scale Candidate genes novel were supported concordance between their observed effects TOU-A biochemical characterization. Local populations complement panels complete architecture traits. article part theme issue 'Genetic basis adaptation speciation: causative mutations'.
Язык: Английский
Процитировано
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