Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(32), С. 17746 - 17761
Опубликована: Июль 30, 2024
In
order
to
solve
the
food
safety
problem
better,
it
is
very
important
develop
a
rapid
and
sensitive
technology
for
detecting
contamination
residues.
Organic
photoelectrochemical
transistor
(OPECT)
biosensor
rely
on
photovoltage
generated
by
semiconductor
upon
excitation
light
regulate
conductivity
of
polymer
channels
realize
analysis
under
zero
gate
bias.
This
integrates
excellent
characteristics
(PEC)
bioanalysis
high
sensitivity
inherent
amplification
ability
organic
electrochemical
(OECT).
Based
this,
OPECT
detection
has
been
proven
be
superior
traditional
methods.
this
review,
we
summarize
research
status
in
disease
markers
residue
analysis,
basic
principle,
classification,
biosensing
mechanism
are
briefly
introduced,
recent
applications
discussed
according
signal
strategy.
We
mainly
introduced
methods
applied
different
fields,
including
hazard
residues
such
as
prostate-specific
antigen,
heart-type
fatty
acid
binding
protein,
T-2
toxin
milk
samples,
fat
mass
objectivity
related
ciprofloxacin
milk.
The
provides
considerable
development
potential
construction
platforms
many
agriculture
food,
hopes
provide
some
reference
future
with
higher
selectivity,
faster
speed
sensitivity.
DNA
aptamers
can
bind
small
molecule
ligands
with
high
affinity
and
specificity
to
produce
a
unique
supramolecular
structure.
Methods
obtain
structural
information
about
the
binding
interaction
coupled
sensitive
diagnostics
is
gold
standard
for
aptasensor
design.
However,
most
sensing
strategies
afford
ligand
detection
without
insight,
while
NMR-
or
crystallography-based
methods
lack
sensitivity
required
diagnostics.
FRET-based
both,
especially
internal
fluorescent
nucleobase
probes
that
are
spatially
fixed
within
helix,
but
dual
aptamer
labeling
compromise
toward
its
target.
Herein,
we
showcase
surrogate-ligand
strategy
affords
target-site
mapping
combined
target
addresses
these
challenges.
A
molecular
rotor
(FMR)
thiophene
chalcone
(Th6HI)
surrogate
was
incorporated
into
tetracycline
(TC)
42-mer
OTC2
serve
as
an
acceptor
TC
donor.
Time-resolved
fluorescence
anisotropy
experiments
predict
compact
prefolded
hardly
impacted
by
binding.
Consequently,
direct
excitation
of
FMR
Th6HI
at
530
nm
little
response
binding,
probe
rigidity
not
strongly
altered.
In
contrast,
indirect
through
donor
378
site-dependent
sensitized
(Fsen)
enhanced
compared
native
platform,
which
utilizes
intrinsic
fluorescence.
Furthermore,
FRET
provides
build
new
model
TC-OTC2
complex
akin
three-helical
structure
hammerhead
ribozyme.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 16, 2025
Multiplexed
and
portable
detection
of
small
molecular
food
contaminants
in
complex
matrices
remains
a
challenge
analytical
chemistry.
Herein,
inspired
by
the
multiplexed
detection,
nucleic-acid-targeting
capability,
programmability
Thermus
thermophilus
argonaute
(TtAgo),
fingerprint
recognition
nanopore
technology,
we
proposed
TtAgo-assisted
encoding
strategy
to
detect
three
mycotoxins
simultaneously
(AFB1,
OTA,
ZEN).
In
this
method,
effective
signal
conversion
turns
into
DNA
reporters
that
were
observed
via
technology
.
general,
guide
DNAs
(gDNAs)
released
presence
mycotoxins,
followed
specific
cleavage
mediated
TtAgo
release
reporters.
The
translocation
produces
highly
distinguishable
information
due
favorable
resolution
nanopore.
Owing
high
nanopore,
method
displayed
low
LODs
(AFB1
67.18
fM,
OTA
13.32
ZEN
11.69
fM)
with
wide
linear
ranges.
Furthermore,
specificity
practical
application
also
verified
showed
satisfactory
results.
conclusion,
offers
novel
avenue
for
molecules.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Ноя. 27, 2024
Cell
ion
channels,
cell
proliferation
and
metastasis,
many
other
life
activities
are
inseparable
from
the
regulation
of
trace
or
even
single
copper
(Cu+
and/or
Cu2+).
In
this
work,
an
electrochemical
sensor
for
sensitive
quantitative
detection
0.4−4
amol
L−1
ions
is
developed
by
adopting:
(1)
catalyzing
click-chemistry
reaction
to
capture
numerous
signal
units;
(2)
special
adsorption
assembly
method
units
ensure
generation
efficiency;
(3)
fast
scan
voltammetry
at
400
V
s−1
enhance
intensity.
And
then,
single-atom
realized
constructing
a
multi-layer
deep
convolutional
neural
network
model
FSVNet
extract
hidden
features
information
voltammograms
0.2
ions.
Here,
we
show
multiple
amplification
strategy
based
on
functionalized
nanomaterials
voltammetry,
together
with
learning
method,
which
realizes
Life
inextricably
linked
authors
report
learning-assisted
click
chemistry
voltammetry.
Chemistry - A European Journal,
Год журнала:
2023,
Номер
29(32)
Опубликована: Апрель 4, 2023
Esterases
enzymes
regulate
the
body's
homeostasis
by
catalyzing
hydrolysis
of
various
esters.
These
are
also
involved
in
protein
metabolism,
detoxification,
and
signal
transmission.
Most
importantly,
esterase
plays
a
significant
role
cell
viability
cytotoxicity
assays.
Hence,
developing
an
efficient
chemical
probe
is
essential
for
monitoring
activity.
Several
fluorescent
probes
have
been
reported
targeting
cytosol
lysosomes.
However,
ability
to
create
constrained
due
lack
understanding
esterase's
active
site
hydrolyzing
substrate.
In
addition,
turn-on
may
limit
monitoring.
Herein,
we
developed
unique
probe,
PM-OAc,
monitor
mitochondrial
enzyme
activity
ratiometrically.
This
exhibited
bathochromic
wavelength
shift
with
alkaline
pH
(pH∼8.0)
intramolecular
charge
transfer
(ICT)
process.
The
phenomenon
well
supported
TD-DFT
calculation.
Moreover,
substrate
(PM-OAc)
binding
at
its
catalytic
mechanism
hydrolyze
ester
bond
elucidated
molecular
dynamics
(MD)
simulation
QM/MM
(Quantum
mechanics/molecular
mechanics)
calculations,
respectively.
Fluorescent
image-based
analysis
cellular
environment
reveals
that
our
can
distinguish
between
live
dead
cells
based
on
Journal of Food and Drug Analysis,
Год журнала:
2022,
Номер
30(4), С. 549 - 561
Опубликована: Ноя. 23, 2022
The
co-occurrence
and
accumulation
of
mycotoxin
in
food
feed
constitutes
a
major
issue
to
safety,
security,
public
health.
Accurate
sensitive
mycotoxins
analysis
can
avoid
toxin
contamination
as
well
reduce
wastage
caused
by
false
positive
results.
This
mini
review
focuses
on
the
recent
advance
detection
methods
for
multiple
mycotoxins,
which
mainly
depends
immunoassay
technologies.
Advance
technologies
integrated
enable
simultaneous
enhance
outcomes’
quality.
It
highlights
toxicogenomic
novel
approach
hazard
assessment
utilizing
computational
map
molecular
events
biological
processes.
Indeed,
is
powerful
tool
understand
health
effects
from
exposure
it
offers
insight
mechanisms
exposures
cause
diseases.
