Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 736, С. 150873 - 150873
Опубликована: Окт. 23, 2024
Язык: Английский
Biomaterials Research, Год журнала: 2023, Номер 27(1)
Опубликована: Фев. 9, 2023
Hyaluronic acid (HA) is widely distributed in human connective tissue, and its unique biological physicochemical properties ability to facilitate structure repair make it a promising candidate for three-dimensional (3D) bioprinting the field of tissue regeneration biomedical engineering. Moreover, HA an ideal raw material bioinks engineering because histocompatibility, non-immunogenicity, biodegradability, anti-inflammatory properties, anti-angiogenic modifiability. Tissue multidisciplinary focusing on vitro reconstructions mammalian tissues, such as cartilage engineering, neural skin other areas that require further clinical applications. In this review, we first describe modification methods, cross-linking strategies derivatives then critically discuss strengths, shortcomings, feasibility each method. Subsequently, reviewed practical applications outcomes bioink 3D bioprinting. Finally, challenges opportunities development provide research references insights.
Язык: Английский
Процитировано
32
Precision medicine and engineering., Год журнала: 2024, Номер 1(1), С. 100001 - 100001
Опубликована: Июль 28, 2024
Stimulus-responsive hydrogels can undergo controllable shape deformation and exhibit sol-gel transition behavior under stimulus signals such as pH, reactive oxygen species (ROS), heat, light. Polysaccharides have become ideal candidates for constructing stimulus-responsive due to their biocompatibility biodegradability. The diversity of molecular weights functional groups polysaccharides allows them self-assemble or cooperate with other materials obtain through physical chemical crosslinking. Bioactive ingredients nanomaterials be conveniently encapsulated in the hydrogel matrix meet various requirements. polysaccharide-based are widely used biomedical field, especially fields drug delivery, tissue engineering, biosensors, imaging. In this review, design strategies recent research advances stimuli-responsive summarized. Future challenges also presented, review will guide study polysaccharide hydrogels.
Язык: Английский
Процитировано
11
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 270, С. 132334 - 132334
Опубликована: Май 12, 2024
Язык: Английский
Процитировано
8
Molecules, Год журнала: 2023, Номер 28(2), С. 809 - 809
Опубликована: Янв. 13, 2023
Confusing low-molecular-weight hyaluronic acid (LMWHA) from degradation and enzymatic hydrolysis (named LMWHA–A LMWHA–E, respectively) will lead to health hazards commercial risks. The purpose of this work is analyze the structural differences between then achieve a fast accurate classification based on near-infrared (NIR) spectroscopy machine learning. First, we combined nuclear magnetic resonance (NMR), Fourier transform infrared (FTIR) spectroscopy, two-dimensional correlated NIR (2DCOS), aquaphotomics LMWHA–E. Second, compared dimensionality reduction methods including principal component analysis (PCA), kernel PCA (KPCA), t-distributed stochastic neighbor embedding (t-SNE). Finally, in effect traditional learning partial least squares–discriminant (PLS-DA), support vector (SVC), random forest (RF) as well deep one-dimensional convolutional neural network (1D-CNN) long short-term memory (LSTM) were compared. results showed that genetic algorithm (GA)–SVC RF best performers learning, but their highest accuracy test dataset was 90%, while 1D-CNN LSTM models training 100%. study show with better for Our research provides new methodological reference rapid biological macromolecules.
Язык: Английский
Процитировано
13
Archives of Microbiology, Год журнала: 2025, Номер 207(3)
Опубликована: Фев. 20, 2025
Язык: Английский
Процитировано
0
World Journal of Microbiology and Biotechnology, Год журнала: 2025, Номер 41(3)
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер 307, С. 142155 - 142155
Опубликована: Март 15, 2025
Язык: Английский
Процитировано
0
Biomedicines, Год журнала: 2025, Номер 13(3), С. 731 - 731
Опубликована: Март 17, 2025
Additive manufacturing, also known as 3D printing, is becoming more and popular because of its wide range materials flexibility in design. Layer by layer, complex structures can be generated the revolutionary computer-aided process bioprinting. It particularly crucial for youngsters elderly patients a useful tool tailored pharmaceutical therapy. A lot research has been carried out recently on use polysaccharides matrices tissue engineering medication delivery. Still, there great need to create affordable, sustainable bioink with high-quality mechanical, viscoelastic, thermal properties well biocompatibility biodegradability. The primary biological substances (biopolymers) chosen formulation are proteins polysaccharides, among several resources utilized creation such structures. These naturally occurring biomaterials give macromolecular structure mechanical qualities (biomimicry), generally compatible tissues cells (biocompatibility), harmonious digesting processes (biodegradability). However, difficulty cell-laden printing technique (bioprinting) rheological characteristics these natural-based bioinks. Polysaccharides widely used they abundant reasonably priced natural polymers. Additionally, serve excipients formulations pharmaceuticals, nutraceuticals, cosmetics. remarkable benefits polysaccharides—biocompatibility, biodegradability, safety, non-immunogenicity, absence secondary pollution—make them ideal substrates. purpose this publication examine recent developments challenges related stimuli-responsive site-specific administration engineering.
Язык: Английский
Процитировано
0
Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Май 8, 2025
Oligosaccharides have the potential to act as prebiotics and can be obtained by enzymatic hydrolysis of polysaccharides. Sphingans are exopolysaccharides, including gellan, welan, diutan, which synthesized genus Sphingomonas. Sphingan lyase (SpnR) has attracted a great deal interest due its ability produce oligosaccharides through degradation sphingans. However, there few reports on catalytic mechanism hydrolysate analysis SpnR. Here, SpnR from Sphingomonas sp. HT-1 was heterologously expressed in Escherichia coli. The optimal temperature pH purified were 35 °C 7.2, respectively. good alkali resistance relatively mild optimum temperature. Na+ Fe3+ increased activities, contrast Zn2+ EDTA. could degrade sphingans, most suitable substrate welan. Analysis cleavage pattern revealed that acts specifically β-1,4-glycosidic bond obtain minimum trisaccharide sphingan (SpnOS). gut microbiota vitro showed SpnOS caused substantial modification intestinal microbiota, characterized an augmented proliferation beneficial bacterial populations, notably Bacteroides Lactobacillus.
Язык: Английский
Процитировано
0
Engineering Microbiology, Год журнала: 2023, Номер 3(4), С. 100086 - 100086
Опубликована: Апрель 6, 2023
Leech hyaluronidase (LHyal) is a hyperactive hyaluronic acid (HA) hydrolase that belongs to the hyaluronoglucuronidase family. Traditionally, LHyal extracted from heads of leeches, but recent development
Язык: Английский
Процитировано
8