Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 19, 2024
CRISPR/Cas12a-based
biosensors
have
garnered
significant
attention
in
the
field
of
point-of-care
testing
(POCT),
yet
majority
CRISPR-based
POCT
methods
employ
fluorescent
systems
as
report
probes.
Herein,
we
a
new
CRISPR/Cas12a-enabled
multicolor
visual
biosensing
strategy
for
rapid
detection
disease
biomarkers.
The
proposed
assay
provided
vivid
color
responses
to
enhance
accuracy
detection.
In
existence
target,
Critical Reviews in Food Science and Nutrition,
Год журнала:
2023,
Номер
64(32), С. 12061 - 12082
Опубликована: Сен. 10, 2023
Food
safety
problems
have
become
one
of
the
most
important
public
health
issues
worldwide.
Therefore,
development
rapid,
effective
and
robust
detection
is
great
importance.
Amongst
a
range
methods,
nucleic
acid
isothermal
amplification
(NAIA)
plays
role
in
food
detection.
However,
widespread
application
remains
limited
due
to
few
shortcomings.
CRISPR/Cas
system
has
emerged
as
powerful
tool
detection,
which
could
be
readily
integrated
with
NAIA
improve
sensitivity,
specificity,
adaptability
versatility
dependability.
currently
there
was
lack
comprehensive
summary
regarding
integration
field
In
this
review,
recent
advances
based
on
CRISPR/Cas-integrated
were
comprehensively
reviewed.
To
begin
with,
summarized.
Then,
types
working
principles
introduced.
The
applications
for
mainly
introduced
objectively
discussed.
Lastly,
current
challenges
future
opportunities
proposed.
summary,
technology
expected
an
approach
leading
safer
more
reliable
industry.
Biological Procedures Online,
Год журнала:
2024,
Номер
26(1)
Опубликована: Июнь 26, 2024
Crimean-Congo
hemorrhagic
fever
virus
(CCHFV)
is
a
tick-borne
virus,
and
zoonosis,
affects
large
regions
of
Asia,
Southwestern
Southeastern
Europe,
Africa.
CCHFV
can
produce
symptoms,
including
no
specific
clinical
mild
to
severe
or
deadly
infections.
Virus
isolation
attempts,
antigen-capture
enzyme-linked
immunosorbent
assay
(ELISA),
reverse
transcription
polymerase
chain
reaction
(RT-PCR)
are
all
possible
diagnostic
tests
for
CCHFV.
Furthermore,
an
efficient,
quick,
cheap
technology,
biosensors,
must
be
designed
developed
detect
The
goal
this
article
offer
overview
modern
laboratory
available
as
well
other
innovative
detection
methods
such
biosensors
CCHFV,
the
benefits
limits
assays.
confirmed
cases
CCHF
managed
with
symptomatic
assistance
general
supportive
care.
This
study
examined
various
treatment
modalities,
their
respective
limitations
developments,
immunotherapy
antivirals.
Recent
biotechnology
advancements
availability
suitable
animal
models
have
accelerated
development
vaccines
by
substantial
margin.
We
range
potential
in
research,
comprising
nucleic
acid,
viral
particles,
inactivated,
multi-epitope
vaccines,
present
obstacles
developments
field.
Thus,
purpose
review
comprehensive
summary
endeavors
dedicated
advancing
diagnostic,
therapeutic,
preventive
strategies
infection
anticipation
forthcoming
hazards.
Microchemical Journal,
Год журнала:
2024,
Номер
199, С. 109993 - 109993
Опубликована: Янв. 24, 2024
Sugarcane
diseases
are
caused
by
various
pathogens,
and
rapid
accurate
identification
of
these
has
many
challenges.
Detection
sugarcane
using
conventional
assays
often
handicapped
their
low-throughput,
high-cost,
low-sensitivity
nature.
Here,
we
report
a
novel
method
for
the
multiplexed
visual
detection
pathogens
CRISPR/Cas12a
coupled
with
labeled
loop-mediated
isothermal
amplification
(Cas-mCfLAMP).
This
labels
product
during
LAMP
amplification,
relieving
high
dependence
on
specific
CrRNAs
enabling
multiple
target
genes
in
single
reaction.
In
addition,
have
developed
field
system
that
allows
without
need
specialized
technicians
or
expensive
equipment.
is
simple,
efficient,
specific,
cost-effective
disease
detection,
holds
potential
to
be
modified
other
plant
diseases.
Foodborne
viruses
are
significant
public
health
threats,
capable
of
causing
life-threatening
infections
and
posing
major
risks
for
future
pandemics.
However,
the
development
vaccines
treatments
remains
limited
due
to
gaps
in
understanding
their
biophysical
properties.
Among
these
viruses,
noroviruses
currently
leading
cause
viral
gastroenteritis
globally
responsible
numerous
foodborne
outbreaks.
In
this
review,
we
explore
use
methods,
with
a
focus
on
atomic
force
microscopy
(AFM),
study
viruses.
We
demonstrate
how
AFM
can
provide
crucial
insights
into
virus-host
interactions,
transmission
dynamics,
environmental
stability.
also
show
that
integration
various
approaches
offers
new
opportunities
advancing
our
ultimately
guiding
effective
prevention
strategies
antiviral
therapies.
Foodborne Pathogens and Disease,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 14, 2025
Human
astrovirus
(HAstV)
and
sapovirus
(SaV)
are
significant
pathogens
associated
with
acute
gastroenteritis
in
humans.
This
study
established
a
triple
reverse
transcription-droplet
digital
polymerase
chain
reaction
(PCR)
(RT-ddPCR)
assay
incorporating
MS2
phage
as
process
control
virus
for
the
simultaneous
quantification
of
HAstV
SaV.
The
was
validated
using
240
bivalve
samples,
comprising
five
shellfish
species:
Ostreidae
(n
=
43),
Ruditapes
philippinarum
84),
Sinonovacula
constricta
27),
Scapharca
subcrenata
23),
Pectinidae
63).
results
indicated
that
developed
RT-ddPCR
had
good
exclusivity,
detection
limits
5.15
copies/reaction
HAstV,
7.71
SaV,
6.13
RNA.
Viral
screening
revealed
1.25%
(3/240)
maximum
load
22,140
copies/2
g,
while
SaV
exhibited
higher
prevalence
13.33%
(32/240)
peak
concentration
68,700
g.
Different
species
varying
rates;
highest
rate
found
Ostreidaes
at
20.93%
(9/43),
followed
by
philippinarums
14.29%
(12/84),
subcrenatas
13.04%
(3/23),
Pectinidaes
11.11%
(7/63),
constrictas
3.70%
(1/27).
only
detected
R.
Pectinidaes,
rates
1.19%
(1/84)
3.17%
(2/63),
respectively.
Additionally,
both
were
single
sample
(0.42%,
1/240).
this
is
reliable,
accurate,
highly
sensitive,
providing
effective
technical
support
quantitative
shellfish.
