Regulation of forkhead box O transcription factor by insulin signaling pathway controls the reproductive diapause of the lady beetle, Coccinella septempunctata
International Journal of Biological Macromolecules,
Год журнала:
2023,
Номер
258, С. 128104 - 128104
Опубликована: Ноя. 17, 2023
Язык: Английский
Unraveling the Stress Response: How Low Temperature and Nanoparticles Impact the Chinese Mitten Crab (Eriocheir sinensis)
Journal of Hazardous Materials,
Год журнала:
2025,
Номер
494, С. 138775 - 138775
Опубликована: Май 29, 2025
Язык: Английский
RNAi-mediated glucose transporter 4 (Glut4) silencing inhibits ovarian development and enhances deltamethrin-treated energy depletion in Locusta migratoria
Pesticide Biochemistry and Physiology,
Год журнала:
2024,
Номер
203, С. 106014 - 106014
Опубликована: Июль 6, 2024
Energy
metabolism
is
essential
for
insect
development,
reproduction
and
detoxification.
Insects
often
reallocate
energy
resources
to
manage
external
stress,
balancing
the
demands
of
detoxification
reproduction.
Glucose
transport
4
(Glut4),
a
glucose
transporter,
involved
in
lipid
metabolism.
However,
specific
molecular
mechanism
Glut4
reproduction,
its
role
response
insecticide-induced
oxidative
stress
remain
unclear.
In
this
study,
LmGlut4
was
identified
analyzed
Locusta
migratoria.
Silencing
significantly
reduced
vitellogenin
(Vg)
biosynthesis
fat
body
Vg
absorption
by
oocytes,
ultimately
hindering
ovarian
development
oocyte
maturation.
Knockdown
also
inhibited
key
hormones,
such
as
juvenile
hormone
(JH),
20-hydroxyecdysone
(20E)
insulin.
Furthermore,
knockdown
led
triglyceride
(TG)
glycogen
content
ovary,
well
decreased
capacity
trehalose
adipocytes.
Additionally,
dsLmGlut4-treated
locusts
showed
heightened
sensitivity
deltamethrin,
leading
increased
depletion
during
This
study
sheds
light
on
biological
function
ovary
provides
potential
target
genes
exploring
pest
management
strategies.
Язык: Английский
RNA interference against the putative insulin receptor substrate gene IRS1 affects growth and development in the pest natural enemy Pardosa pseudoannulata
Pest Management Science,
Год журнала:
2023,
Номер
80(2), С. 648 - 660
Опубликована: Сен. 27, 2023
Insulin
signalling
pathways
play
crucial
roles
in
regulating
growth
and
development
insects,
but
their
effects
on
the
of
Arachnids,
such
as
spiders,
have
rarely
been
studied.
As
a
valuable
pest
natural
enemy
agricultural
fields,
molecular
mechanisms
insulin
pathway-mediated
wolf
spider,
Pardosa
pseudoannulata,
are
particular
interest.
Язык: Английский
RNAi-Mediated FoxO Silencing Inhibits Reproduction in Locusta migratoria
Insects,
Год журнала:
2024,
Номер
15(11), С. 891 - 891
Опубликована: Ноя. 14, 2024
is
a
downstream
target
gene
of
cellular
nutrient
and
growth
factors,
oxidative
stress
responses,
insulin
signaling
pathways.
It
play
crucial
role
in
insect
growth,
development,
reproduction.
Язык: Английский
Functions of Insulin-like Peptide Genes (CsILP1 and CsILP2) in Female Reproduction of the Predatory Ladybird Coccinella septempunctata (Coleoptera: Coccinellidae)
Insects,
Год журнала:
2024,
Номер
15(12), С. 981 - 981
Опубликована: Дек. 11, 2024
Insulin-like
peptides
(ILPs)
are
important
peptide
hormones
in
insects,
particularly
involved
regulating
physiological
processes
such
as
growth,
development,
and
reproduction.
However,
the
specific
roles
of
ILPs
reproduction
natural
enemy
insects
remain
unknown.
In
this
study,
two
ILP
genes,
CsILP1
CsILP2,
were
cloned
their
functions
analyzed
female
Coccinella
septempunctata
L.
(Coleoptera:
Coccinellidae).
The
open
reading
frames
(ORFs)
CsILP2
384
bp
357
bp,
respectively.
expression
increased
on
6th
day
after
eclosion,
reaching
its
peak
12th
day,
while
levels
showed
a
significant
increase
then
stabilized.
different
tissues,
was
highly
expressed
ovaries,
predominated
elytra.
Injection
dsRNA
targeting
resulted
down-regulation
insulin
pathway
genes.
relative
ovarian
development-related
genes
Vasa,
G2/M,
Vg
reduced
by
82.50%,
89.55%.
96.98%
dsCsILP1-treated
females,
42.55%,
91.36%,
55.63%
dsCsILP2-treated
females.
Furthermore,
substantial
decreases
14-day
fecundity
observed,
with
reductions
89.99%
for
dsCsILP1
83.45%
dsCsILP2.
These
results
confirm
regulatory
C.
Язык: Английский