Functions of Insulin-like Peptide Genes (CsILP1 and CsILP2) in Female Reproduction of the Predatory Ladybird Coccinella septempunctata (Coleoptera: Coccinellidae) DOI Creative Commons
Shanshan Feng, Da Wang,

Qiuju Qin

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(12), P. 981 - 981

Published: Dec. 11, 2024

Insulin-like peptides (ILPs) are important peptide hormones in insects, particularly involved regulating physiological processes such as growth, development, and reproduction. However, the specific roles of ILPs reproduction natural enemy insects remain unknown. In this study, two ILP genes, CsILP1 CsILP2, were cloned their functions analyzed female Coccinella septempunctata L. (Coleoptera: Coccinellidae). The open reading frames (ORFs) CsILP2 384 bp 357 bp, respectively. expression increased on 6th day after eclosion, reaching its peak 12th day, while levels showed a significant increase then stabilized. different tissues, was highly expressed ovaries, predominated elytra. Injection dsRNA targeting resulted down-regulation insulin pathway genes. relative ovarian development-related genes Vasa, G2/M, Vg reduced by 82.50%, 89.55%. 96.98% dsCsILP1-treated females, 42.55%, 91.36%, 55.63% dsCsILP2-treated females. Furthermore, substantial decreases 14-day fecundity observed, with reductions 89.99% for dsCsILP1 83.45% dsCsILP2. These results confirm regulatory C.

Language: Английский

Regulation of forkhead box O transcription factor by insulin signaling pathway controls the reproductive diapause of the lady beetle, Coccinella septempunctata DOI
Junjie Chen, Xiaoxiao Liu,

Penghui Guo

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 258, P. 128104 - 128104

Published: Nov. 17, 2023

Language: Английский

Citations

9
Unraveling the Stress Response: How Low Temperature and Nanoparticles Impact the Chinese Mitten Crab (Eriocheir sinensis) DOI
Zihan Zhou,

Tian Jian,

Si Qin

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 494, P. 138775 - 138775

Published: May 29, 2025

Language: Английский

Citations

0
RNAi-mediated glucose transporter 4 (Glut4) silencing inhibits ovarian development and enhances deltamethrin-treated energy depletion in Locusta migratoria DOI Creative Commons
Mingjun Wang, Ningxin Yang,

Wenhui Guo

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 203, P. 106014 - 106014

Published: July 6, 2024

Energy metabolism is essential for insect development, reproduction and detoxification. Insects often reallocate energy resources to manage external stress, balancing the demands of detoxification reproduction. Glucose transport 4 (Glut4), a glucose transporter, involved in lipid metabolism. However, specific molecular mechanism Glut4 reproduction, its role response insecticide-induced oxidative stress remain unclear. In this study, LmGlut4 was identified analyzed Locusta migratoria. Silencing significantly reduced vitellogenin (Vg) biosynthesis fat body Vg absorption by oocytes, ultimately hindering ovarian development oocyte maturation. Knockdown also inhibited key hormones, such as juvenile hormone (JH), 20-hydroxyecdysone (20E) insulin. Furthermore, knockdown led triglyceride (TG) glycogen content ovary, well decreased capacity trehalose adipocytes. Additionally, dsLmGlut4-treated locusts showed heightened sensitivity deltamethrin, leading increased depletion during This study sheds light on biological function ovary provides potential target genes exploring pest management strategies.

Language: Английский

Citations

2
RNA interference against the putative insulin receptor substrate gene IRS1 affects growth and development in the pest natural enemy Pardosa pseudoannulata DOI Open Access

Xuelai Li,

Wei Li, Shichang Zhang

et al.

Pest Management Science, Journal Year: 2023, Volume and Issue: 80(2), P. 648 - 660

Published: Sept. 27, 2023

Insulin signalling pathways play crucial roles in regulating growth and development insects, but their effects on the of Arachnids, such as spiders, have rarely been studied. As a valuable pest natural enemy agricultural fields, molecular mechanisms insulin pathway-mediated wolf spider, Pardosa pseudoannulata, are particular interest.

Language: Английский

Citations

2
RNAi-Mediated FoxO Silencing Inhibits Reproduction in Locusta migratoria DOI Creative Commons
Jiaying Xu, Zhen Yuan, Huazhang Zhao

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(11), P. 891 - 891

Published: Nov. 14, 2024

is a downstream target gene of cellular nutrient and growth factors, oxidative stress responses, insulin signaling pathways. It play crucial role in insect growth, development, reproduction.

Language: Английский

Citations

0
Functions of Insulin-like Peptide Genes (CsILP1 and CsILP2) in Female Reproduction of the Predatory Ladybird Coccinella septempunctata (Coleoptera: Coccinellidae) DOI Creative Commons
Shanshan Feng, Da Wang,

Qiuju Qin

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(12), P. 981 - 981

Published: Dec. 11, 2024

Insulin-like peptides (ILPs) are important peptide hormones in insects, particularly involved regulating physiological processes such as growth, development, and reproduction. However, the specific roles of ILPs reproduction natural enemy insects remain unknown. In this study, two ILP genes, CsILP1 CsILP2, were cloned their functions analyzed female Coccinella septempunctata L. (Coleoptera: Coccinellidae). The open reading frames (ORFs) CsILP2 384 bp 357 bp, respectively. expression increased on 6th day after eclosion, reaching its peak 12th day, while levels showed a significant increase then stabilized. different tissues, was highly expressed ovaries, predominated elytra. Injection dsRNA targeting resulted down-regulation insulin pathway genes. relative ovarian development-related genes Vasa, G2/M, Vg reduced by 82.50%, 89.55%. 96.98% dsCsILP1-treated females, 42.55%, 91.36%, 55.63% dsCsILP2-treated females. Furthermore, substantial decreases 14-day fecundity observed, with reductions 89.99% for dsCsILP1 83.45% dsCsILP2. These results confirm regulatory C.

Language: Английский

Citations

0