Biodegradation of chemical contamination by lactic acid bacteria: A biological tool for food safety

Food Chemistry, Год журнала: 2024, Номер 460, С. 140732 - 140732

Опубликована: Авг. 2, 2024

Язык: Английский

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Designing ASSMD Strategy for Exploring and Engineering Extreme Thermophilic Ancestral Nitrilase for Nitriles Biocatalysis

ACS Catalysis, Год журнала: 2024, Номер 14(18), С. 13825 - 13838

Опубликована: Сен. 3, 2024

Enzyme thermostability is vital for prolonged reactions and reusability. Extremozymes, known their high thermal stability, have gained biocatalysis prominence. Extremozymes evolve under extreme conditions, possibly becoming extinct during the evolutionary process of adapting to current environment. Fortunately, ancestral enzyme sequence reconstruction could deduce existing enzymes through computer algorithms. Here, we designed an sequence-structure-molecule dynamics (ASSMD) strategy unveil molecular insights into in landscape. Furthermore, this approach was applied explore extremophilic nitrilase. In a dynamic flexibility trough, obtained ASR135, nitrilase capable tolerating 90 °C. Combining evolution analysis laboratory evolution, achieved further ASR135 event mutant ASR135-M4 (S97E/S101A/N124H/H155Y), which exhibited hydrolytic activity at 100 Mechanistic revealed that addition salt bridge, hydrogen bond, π-alkyl interaction tetrahedral cage strengthening hydrophobic core inside protein. These modifications resulted more robust network between four secondary structures. general, ASSMD holds potential discovering high-performance nitrilases, particularly extremozymes. Additionally, sheds light on enzyme-directed mechanisms.

Язык: Английский

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A novel glycosyltransferase from Bacillus subtilis achieves zearalenone detoxification by diglycosylation modification

Food & Function, Год журнала: 2024, Номер 15(11), С. 6042 - 6053

Опубликована: Янв. 1, 2024

A strain of Bacillus subtilis YQ-1 was screened from corn soil, which can convert ZEN into C 19 H 26 O 8 and diglucoside, a new glycosyltransferase mined that catalyze the glycosylation achieve effective detoxification ZEN.

Язык: Английский

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Mycotoxin Biodegradation by Bacillus Bacteria—A Review

Toxins, Год журнала: 2024, Номер 16(11), С. 478 - 478

Опубликована: Ноя. 4, 2024

Mycotoxins are toxic secondary metabolites produced by various types of fungi that known to contaminate food products; their presence in the chain poses significant risks human and animal health leads enormous economic losses feed industry worldwide. Ensuring safety quality detoxifying mycotoxin is therefore paramount importance. Several procedures control fungal toxins have been extensively investigated, such as preventive measures, physical chemical methods, biological strategies. In recent years, microbial degradation mycotoxins has attracted much attention due its reliability, efficiency, cost-effectiveness. Notably, bacterial species from Bacillus genus emerged promising candidates for decontamination owing diverse metabolic capabilities resilience harsh environmental conditions. This review manuscript aims provide a summary studies on biodegradation bacteria, thereby illustrating potential applications development mycotoxin-degrading products.

Язык: Английский

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Biodegradation Characteristics and Mechanism of Aflatoxin B₁ by Bacillus amyloliquefaciens from Enzymatic and Multiomics Perspectives

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(28), С. 15841 - 15853

Опубликована: Июль 3, 2024

Aflatoxin B

Язык: Английский

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A novel zearalenone lactonase can effectively mitigate zearalenone-induced reproductive toxicity in gilts

Toxicon, Год журнала: 2025, Номер unknown, С. 108257 - 108257

Опубликована: Янв. 1, 2025

Язык: Английский

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Characterization, mechanism, and application of aldolase: a patulin-degrading enzyme from Kluyveromyces Marxianus YG-4

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106345 - 106345

Опубликована: Март 1, 2025

Язык: Английский

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Genome mining of an endophytic natural yeast isolate Rhodotorula sp. Y090 and production of the antioxidant ergothioneine

Journal of Biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Comparative investigation of Mycotoxin detoxification mechanisms by lactic acid bacteria (Lactobacillus species) and non-lactic acid bacteria

Journal of Food Measurement & Characterization, Год журнала: 2025, Номер unknown

Опубликована: Апрель 26, 2025

Язык: Английский

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Innovative probiotic fermentation approach for zearalenone detoxification in dried distiller’s grains

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Июнь 2, 2025

Zearalenone (ZEN) contamination in dried distiller’s grains and solubles (DDGS) poses serious health risks economic losses animal farming. This study aimed to evaluate the effectiveness of probiotic fermentation using Lactobacillus plantarum CN1 detoxifying ZEN optimizing conditions for maximum efficiency. L. CN1, identified with 99% genetic homology, was used DDGS fermentation. The detoxification mechanism analyzed through adsorption assays, post-heat treatment effects, scanning electron microscopy (SEM). removal assessed over 72 h under various conditions, including bacterial concentration, temperature, pH optimization. results showed that achieved a rate 69% within h, an optimized efficiency 75.6% at 4 × 10 9 CFU/mL. Over 60% adsorbed by cell wall, while supernatant intracellular fluid remained below 5%. Scanning (SEM) analysis highlighted structural changes cells, particularly elongation thinning, more pronounced damage observed following heat treatment. These modifications may explain varying efficiencies observed. Heat treatment, autoclaving, significantly enhanced 82.9%, whereas acid alkali treatments reduced it. Fermentation also improved nutritional quality DDGS, increasing crude protein 7.16%, reducing fiber 0.65%, lowering 4.3. findings demonstrate offers promising, cost-effective strategy mitigating enhancing quality. Future studies should explore large-scale applications potential multi-mycotoxin further improve feed safety.

Язык: Английский

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