Engineering Escherichia coli for Efficient De Novo Synthesis of Salidroside DOI
Weizhu Zeng, Huijing Wang, Jianbin Chen

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 12, 2024

Salidroside is a high-value plant-derived glycoside with diverse biological activities, but the main industrial salidroside production method, extraction from

Язык: Английский

Rational enzyme design by reducing the number of hotspots and library size DOI

Zongmin Qin,

Bo Yuan, Ge Qu

и другие.

Chemical Communications, Год журнала: 2024, Номер 60(76), С. 10451 - 10463

Опубликована: Янв. 1, 2024

Biocatalysts that are eco-friendly, sustainable, and highly specific have great potential for applications in the production of fine chemicals, food, detergents, biofuels, pharmaceuticals, more.

Язык: Английский

Процитировано

6
Biosynthesis of gastrodin via multi-module UDPG supply and site-directed mutagensis of glycosyltransferase in Escherichia coli DOI
Zhao Zhang, Xinying Wang,

Xin Sha

и другие.

Molecular Catalysis, Год журнала: 2025, Номер 578, С. 114953 - 114953

Опубликована: Март 7, 2025

Язык: Английский

Процитировано

0
An efficient C-glycoside production platform enabled by rationally tuning the chemoselectivity of glycosyltransferases DOI Creative Commons
Min Li, Yang Zhou,

Zexing Wen

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Окт. 15, 2024

Despite the broad potential applications of C-glycosides, facile synthetic methods remain scarce. Transforming glycosyltransferases with promiscuous or natural O-specific chemoselectivity to C-glycosyltransferases is challenging. Here, we employ rational directed evolution glycosyltransferase MiCGT generate MiCGT-QDP and MiCGT-ATD mutants which either enhance C-glycosylation switch O-glycosylation, respectively. Structural analysis computational simulations reveal that substrate binding mode govern C-/O-glycosylation selectivity. Notably, mechanism pinpoint crucial residues dictating mode, enabling design four enzymes superior non-inherent chemoselectivity, despite limited sequence homology. Moreover, our best undergo testing 34 substrates, demonstrating superb chemoselectivities, regioselectivities, activities. Remarkably, three C-glycosides an O-glycoside are produced on a gram scale, practical utility. This work establishes highly selective platform for diverse glycosides, offers strategy creating various types glycosylation platforms access pharmaceutically medicinally interesting products.

Язык: Английский

Процитировано

1
Engineering Escherichia coli for Efficient De Novo Synthesis of Salidroside DOI
Weizhu Zeng, Huijing Wang, Jianbin Chen

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 12, 2024

Salidroside is a high-value plant-derived glycoside with diverse biological activities, but the main industrial salidroside production method, extraction from

Язык: Английский

Процитировано

1