Expanding the application of tyrosine: engineering microbes for the production of tyrosine and its derivatives DOI Creative Commons
Jian Shen,

Pengfu Liu,

Bin Zhang

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Апрель 24, 2025

Aromatic compounds are widely used in the fields of medicine, chemical industry, and food, with a considerable market size. Tyrosine, an aromatic amino acid, boasts not only wide range applications but also serves as valuable precursor for synthesizing diverse array high-value compounds. Amid growing concerns over environmental resource challenges, adoption green, clean, sustainable biotechnology producing is gaining increasing recognition viable alternative to traditional synthesis plant extraction methods. This article provides overview current status tyrosine biomanufacturing explores methods generating derivatives, including resveratrol, levodopa, p-coumaric caffeic zosteric tyrosol, hydroxytyrosol, tanshinol, naringenin, eriodictyol, salidroside, using primary raw material. Furthermore, this review examines challenges outlines future directions microbial fermentation production its derivatives.

Язык: Английский

Engineering of Glycosyltransferase for Efficient Biosynthesis of Salidroside DOI
Fucheng Zhu,

Zixu Yan,

Kexue Zhao

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

Salidroside has been widely used in the cosmetic and medicinal industries. Previously, potential glycosyltransferase UGTBS was obtained for salidroside synthesis. However, catalytic efficiency undesirable. In this study, a semirational design applied to engineer UGTBS. The quadruple mutant M4 (I62N/S129T/F168W/Y316S) showed significantly enhanced A conversion rate of 94.7% using M4, which 2.2-fold higher than that wild-type. regioselectivity toward tyrosol hydroxyl also improved, resulting 97.9% total product, 1.2-fold wild type. Kinetic constants molecular simulations indicated increased affinity altered conformation binding pocket accounted Furthermore, fed-batch cascade reaction strategy used, over 183 mM obtained. engineered demonstrated precise catalysis formation.

Язык: Английский

Процитировано

0
Expanding the application of tyrosine: engineering microbes for the production of tyrosine and its derivatives DOI Creative Commons
Jian Shen,

Pengfu Liu,

Bin Zhang

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Апрель 24, 2025

Aromatic compounds are widely used in the fields of medicine, chemical industry, and food, with a considerable market size. Tyrosine, an aromatic amino acid, boasts not only wide range applications but also serves as valuable precursor for synthesizing diverse array high-value compounds. Amid growing concerns over environmental resource challenges, adoption green, clean, sustainable biotechnology producing is gaining increasing recognition viable alternative to traditional synthesis plant extraction methods. This article provides overview current status tyrosine biomanufacturing explores methods generating derivatives, including resveratrol, levodopa, p-coumaric caffeic zosteric tyrosol, hydroxytyrosol, tanshinol, naringenin, eriodictyol, salidroside, using primary raw material. Furthermore, this review examines challenges outlines future directions microbial fermentation production its derivatives.

Язык: Английский

Процитировано

0