Integrated multi-omics analysis of the microbial profile characteristics associated with pulmonary arterial hypertension in congenital heart disease DOI Creative Commons

Jiahui Xie,

Xiaoyu Zhang, Liming Cheng

и другие.

Microbiology Spectrum, Год журнала: 2024, Номер unknown

Опубликована: Окт. 29, 2024

ABSTRACT Dysregulation of immune and inflammatory cells around blood vessels metabolic dysfunction are key mechanisms in the development pulmonary arterial hypertension (PAH). The homeostasis human microbiome plays a crucial role regulating responses progression diseases. For associated with congenital heart disease involving body-lung shunt (PAH-CHD), potential impact on “gut-lung axis” remains underexplored. This study recruited 15 healthy individuals patients due to from Fuwai Yunnan Hospital, Chinese Academy Medical Sciences, Kunming Children's Hospital. We performed differential analyses metabolites microbiota both gut lower respiratory tract for these two groups. goal was investigate metabolome profiles children PAH-CHD analyze interrelationships between profiles. Ultimately, we aim propose value aiding diagnosis. results indicated that characterized by an increased abundance beneficial symbionts, which closely linked changes metabolome. Metabolite functional enrichment analysis revealed energy metabolism reprogramming group, active pathways bile acid secretion carnitine homeostasis. Moreover, expression correlated right function growth development. IMPORTANCE Previous studies have primarily focused relationship PAH. However, microbial perspective gut-lung axis has not been adequately elucidated. Our utilizes integrated multi-omics approach report characteristics lung reference subjects. found influence pathological manifestations through their activity. Additionally, alterations ecological structure. findings suggest modulating composition may positive implications maintaining environment PAH-CHD.

Язык: Английский

Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An exploratory study DOI
Angela Maione,

Marianna Imparato,

Annalisa Buonanno

и другие.

Journal of Functional Foods, Год журнала: 2025, Номер 125, С. 106666 - 106666

Опубликована: Янв. 8, 2025

Язык: Английский

Процитировано

1

Vitamin K2 alleviates dextran sulfate sodium-induced colitis via inflammatory responses, gut barrier integrity, and the gut microbiota in mice DOI

Huakai Wang,

Zhen Liu, Kai Zhan

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 280, С. 136091 - 136091

Опубликована: Сен. 30, 2024

Язык: Английский

Процитировано

3

Candida utilis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice via NF-κB/MAPK Suppression and Gut Microbiota Modulation DOI Open Access

Rongxin Zang,

Zhouliang Liu,

Huihao Wu

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1993 - 1993

Опубликована: Фев. 25, 2025

Candida utilis (CUM) possesses various biological effects, including anti-inflammatory, intestinal microbiota regulatory, and immunomodulatory activities. However, there has been little exploration regarding the effects of CUM on ulcerative colitis (UC). Therefore, this study aimed to investigate beneficial alleviating dextran sulfate sodium (DSS)-induced UC in mice explore potential underlying mechanisms. Here, effect was analyzed using a DSS-induced mouse model (n = 9), results which indicated decrease disease activity index (DAI) mice. Furthermore, alleviated colon shortening, minimized tissue damage, preserved tight junction proteins (Claudin-3, Occludin, ZO-1). reduced level pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), inhibited activation NF-ĸB, MAPK PPARγ signaling pathways, decreased oxidative mediators (MPO, SOD MDA) Additionally, it mitigated dysbiosis by increasing abundance Prevotellaceae Lactobacillus while decreasing Bacteroidaceae Enterobacteriaceae. short-chain fatty acids (SCFAs) content The above provide scientific basis for CUM, as natural supplement, restore balance gut inflammatory promote health.

Язык: Английский

Процитировано

0

Unveiling the Multifaceted Benefits of Simiao Pill in Ulcerative Colitis: Integrative Analysis of Signaling Pathways, Gut Microbiota, and Lipid Metabolism DOI

XingJu Peng,

Qiuping Zou,

Chao Yang

и другие.

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119714 - 119714

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Effect of 1-Kestose on Lipid Metabolism in a High-Fat-Diet Rat Model DOI Open Access

Kento Kuramitsu,

Masayoshi Kubo,

Felicia Cindy

и другие.

Nutrients, Год журнала: 2025, Номер 17(8), С. 1362 - 1362

Опубликована: Апрель 16, 2025

Objectives: Hyperlipidemia is a risk factor for various diseases. Identifying food components that can help reduce the levels of blood lipids, such as cholesterol and triglycerides, global research priority. It has been reported 1-Kestose, fructooligosaccharide, triglyceride in rats; however, underlying mechanisms remain unclear. Therefore, we aimed to elucidate effects 1-kestose supplementation on lipid metabolism gut environment rats. Methods: Twenty male Sprague–Dawley rats (age 8 weeks) were provided 1-kestose-containing water maintained two weeks. After dissection, components, hepatic gene expression, microbiota, bile acid composition cecal contents analyzed. Results: The 1-Kestose intake reduced plasma levels. Additionally, an increase cytochrome P450 family 7 subfamily A member 1 mRNA key synthesis liver, decrease synthesis-related expression observed. In cecum, deconjugated acids, which are involved regulation synthesis, increased. Furthermore, altered microbiota leading abundance specific bacteria, Bifidobacterium, deconjugation conjugated acids. Conclusions: alters potentially influencing host.

Язык: Английский

Процитировано

0

Harnessing Probiotics: Emerging Strategies for Health and Disease Management DOI

Bhargav Parmar,

Riteshkumar Arya, Gayatri Patel

и другие.

Indian Journal of Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Язык: Английский

Процитировано

0

OVA-Induced Food Allergy Leads to Neurobehavioral Changes in Mice and the Potential Role of Gut Microbiota and Metabolites Dysbiosis DOI Open Access
Song Hu, Chunyan Zhou, Yue Zhang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4760 - 4760

Опубликована: Май 16, 2025

The neurobehavioral changes in food allergy mice have not been comprehensively studied, and the mechanism underlying them remains unclear. Our study aims to fully investigate OVA (ovalbumin)-sensitized explore potential via gut microbiota-brain axis. We established mouse (C57BL/6J male) model with OVA, evaluating anaphylactic symptoms levels of Th2 signature cytokine allergy-related antibodies serum. Using behavioral tests, we measured anxiety, depression, social behavior, repetitive attention, spatial memory control mice. In addition, analyzed prefrontal cortex for measuring inflammation-related indicators gathered serum untargeted metabolomics analysis feces 16S rDNA sequencing. exhibited significantly elevated IgE levels. addition anxiety-like, depression-like, behaviors, also displayed less interest damaged attention. TNF-α, IL-1β, IL-6 activation microglia were increased, which might explain neuronal damage. multi-omics technology, amino acid metabolism disruption, particularly carboxylic acids derivatives, was observed mice, remarkably correlated altered abundance microbiota related allergy. Behaviors OVA-induced extensively impaired. disruption associated dysbiosis play a pivotal role impairing neural immune homeostasis damage, could be responsible abnormalities.

Язык: Английский

Процитировано

0

Multi-omics analysis reveals a feedback loop amplifying immune responses in acute graft-versus-host disease due to imbalanced gut microbiota and bile acid metabolism DOI Creative Commons
Lijie Han,

Xianlei Sun,

Jingjing Kong

и другие.

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Авг. 7, 2024

Acute graft-versus-host disease (aGVHD) is primarily driven by allogeneic donor T cells associated with an altered composition of the host gut microbiome and its metabolites. The severity aGVHD after hematopoietic stem cell transplantation (allo-HSCT) not solely determined characteristics; however, underlying mechanisms remain unclear. Using single-cell RNA sequencing, we decoded immune atlas 12 patients who underwent allo-HSCT: six non-aGVHD. We performed a fecal microbiota (16SrRNA sequencing) analysis to investigate bacterial 82 patients: 30 52 Fecal samples from these were analyzed for bile acid metabolism. Through multi-omic analysis, identified feedback loop involving "immune cell-gut microbes-bile metabolites" contributing heightened responses in aGVHD. dysbiosis disruption metabolism contributed exaggerated interleukin-1 mediated response. Our findings suggest that resistin defensins are crucial mitigating against Therefore, comprehensive incorporating cells, microbes, metabolites was developed this study used propose novel, non-immunosuppressive approaches prevent

Язык: Английский

Процитировано

1

Potential of Fiber and Probiotics to Fight Against the Effects of PhIP + DSS-Induced Carcinogenic Process of the Large Intestine DOI Creative Commons
Aida Zapico, Nuria Salazar, Silvia Arboleya

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(45), С. 25161 - 25172

Опубликована: Окт. 29, 2024

We determined the in vivo counteracting effect of fiber and probiotic supplementation on colonic mucosal damage alterations gut microbiota caused by 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP) sodium dextran sulfate (DSS). Male Fischer-344 rats were randomly divided into 4 groups: control (standard diet), PhIP + DSS group diet DSS), (fiber (probiotic DSS). The intake for 3 weeks induced erosion, crypt loss, inflammation, distal colon was more severely damaged. Fiber alleviated reducing loss while increased length. fecal relative abundance Clostridia UCG014 along intervention, contrast to lower abundances these taxa found after administration supplemented with probiotics or fiber. mitigated histological shifting toward a reduction pro-inflammatory taxa.

Язык: Английский

Процитировано

1

Integrated multi-omics analysis of the microbial profile characteristics associated with pulmonary arterial hypertension in congenital heart disease DOI Creative Commons

Jiahui Xie,

Xiaoyu Zhang, Liming Cheng

и другие.

Microbiology Spectrum, Год журнала: 2024, Номер unknown

Опубликована: Окт. 29, 2024

ABSTRACT Dysregulation of immune and inflammatory cells around blood vessels metabolic dysfunction are key mechanisms in the development pulmonary arterial hypertension (PAH). The homeostasis human microbiome plays a crucial role regulating responses progression diseases. For associated with congenital heart disease involving body-lung shunt (PAH-CHD), potential impact on “gut-lung axis” remains underexplored. This study recruited 15 healthy individuals patients due to from Fuwai Yunnan Hospital, Chinese Academy Medical Sciences, Kunming Children's Hospital. We performed differential analyses metabolites microbiota both gut lower respiratory tract for these two groups. goal was investigate metabolome profiles children PAH-CHD analyze interrelationships between profiles. Ultimately, we aim propose value aiding diagnosis. results indicated that characterized by an increased abundance beneficial symbionts, which closely linked changes metabolome. Metabolite functional enrichment analysis revealed energy metabolism reprogramming group, active pathways bile acid secretion carnitine homeostasis. Moreover, expression correlated right function growth development. IMPORTANCE Previous studies have primarily focused relationship PAH. However, microbial perspective gut-lung axis has not been adequately elucidated. Our utilizes integrated multi-omics approach report characteristics lung reference subjects. found influence pathological manifestations through their activity. Additionally, alterations ecological structure. findings suggest modulating composition may positive implications maintaining environment PAH-CHD.

Язык: Английский

Процитировано

0