Fluxomic, Metabolomic, and Transcriptomic Analyses Reveal Metabolic Responses to Phenazine-1-carboxamide Synthesized in Pseudomonas chlororaphis DOI
Ruilian Yao, Peter Y. Lu, Yiling Liu

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(43), С. 23928 - 23936

Опубликована: Окт. 18, 2024

Phenazine-1-carboxamide (PCN) has been exploited as a successful biopesticide due to its broad-spectrum antifungal activity. We engineered PCN-overproducing Pseudomonas chlororaphis strain through overexpressing shikimate pathway genes (aroB, aroQ, aroE, and phzC) deleting negative regulatory (relA, fleQ, pykF). The optimized produced 1.92 g/L PCN with yield of 0.11 g/g glycerol, the highest titer ever reported by using minimal media. To gain deeper insights into underlying network, final parental were examined three distinct omic data sets. 13C-metabolic flux analysis revealed substantial reconfiguration in strain, including activation EDEMP cycle, PP pathway, glyoxylate shunt, pathway. Metabolomic results indicated that central carbon was rerouted Transcriptomic identified global gene expression changes. This study forms basis for further engineering strains achieve outstanding performance.

Язык: Английский

p-AAB/MXene as a novel adsorbent and SALDI matrix for highly efficient enrichment and rapid MS detection of emerging environmental organic pollutants in beverages and PM2.5 DOI
Xu Zhang,

Xiangbo Ji,

Zifang Peng

и другие.

Journal of Chromatography A, Год журнала: 2025, Номер 1745, С. 465759 - 465759

Опубликована: Фев. 8, 2025

Язык: Английский

Процитировано

0

Discovery of novel 3-phenylhydrazone coumarin derivatives as potential antifungal agents against phytopathogenic fungi DOI
Yufei Li, Shanshan Yang, Shun Zhang

и другие.

Food Chemistry, Год журнала: 2025, Номер 486, С. 144655 - 144655

Опубликована: Май 10, 2025

Язык: Английский

Процитировано

0

Discovery of Coumarin-7-N/O-Acethydrazide Derivatives as Novel Antifungal and Herbicidal Agents DOI
Yufei Li, Shanshan Yang, Yifan Zheng

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Июнь 3, 2025

Natural products (NPs) have attracted increasing attention due to their structural diversity, abundance, and low environmental impact, making them valuable for novel fungicide development. In this study, a series of coumarin-7-N/O acethydrazide derivatives were synthesized by incorporating acetic acid moieties into the coumarin scaffold. The in vitro antifungal activities these compounds evaluated against six common plant pathogenic fungi. Compound B13 demonstrated significant effects, with EC50 values 3.24, 1.89, 1.70, 0.36, 4.27, 1.50 μg/mL Botrytis cinerea, Alternaria solani, Fusarium graminearum, Rhizoctonia Colletotrichum orbiculare alternata, respectively. Notably, activity R. solani was comparable commercial boscalid (EC50 = 0.31 μg/mL), while A. alternata significantly higher than that 13.60 2.55 μg/mL, respectively), Furthermore, vivo assays protective efficacy at 200 (86.9%) Validamycin (92.1%). inhibitory effects further confirmed through scanning electron microscopy fluorescence microscopy. influence charge distribution compound on its investigated using Density Functional Theory (DFT). Meanwhile, some exhibited herbicidal four field weeds. These results indicate is promising candidate crop protection, exhibiting high toxicity.

Язык: Английский

Процитировано

0

Novel Aminocoumarin‐Based Schiff Bases: High Antifungal Activity in Agriculture DOI
Xin Zhang, Ming Gao, Yajie Dong

и другие.

Chemistry & Biodiversity, Год журнала: 2024, Номер 21(12)

Опубликована: Авг. 22, 2024

Abstract Structural modification is an effective way to improve the antifungal activity of natural products and has been widely used in development novel fungicides. In this work, a series aminocoumarin‐based Schiff bases were synthesized characterized by 1 H‐NMR, 13 C NMR HR‐MS spectra. The vitro inhibition all compounds was tested against four phytopathogenic fungi ( Alternaria solani , Fusarium oxysporum Botrytis cinerea alternata ) using mycelial growth rate method. results showed that most target exhibited significant activities. particular, 5b 5c 5d 5h 5n 7c 7n 7p more than commercially available fungicides, chlorothalonil azoxystrobin. structure‐activity relationship revealed electron‐withdrawing groups with electronegativity introduced at C‐3 position improving inhibitory halogenated benzaldehydes would be necessary preparation bases. compound (EC 50 =8.73 μg/mL) =26.25 much better positive controls (chlorothalonil azoxystrobin). Therefore, could serve as promising lead for broad‐spectrum which useful applications agriculture.

Язык: Английский

Процитировано

1

Fluxomic, Metabolomic, and Transcriptomic Analyses Reveal Metabolic Responses to Phenazine-1-carboxamide Synthesized in Pseudomonas chlororaphis DOI
Ruilian Yao, Peter Y. Lu, Yiling Liu

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(43), С. 23928 - 23936

Опубликована: Окт. 18, 2024

Phenazine-1-carboxamide (PCN) has been exploited as a successful biopesticide due to its broad-spectrum antifungal activity. We engineered PCN-overproducing Pseudomonas chlororaphis strain through overexpressing shikimate pathway genes (aroB, aroQ, aroE, and phzC) deleting negative regulatory (relA, fleQ, pykF). The optimized produced 1.92 g/L PCN with yield of 0.11 g/g glycerol, the highest titer ever reported by using minimal media. To gain deeper insights into underlying network, final parental were examined three distinct omic data sets. 13C-metabolic flux analysis revealed substantial reconfiguration in strain, including activation EDEMP cycle, PP pathway, glyoxylate shunt, pathway. Metabolomic results indicated that central carbon was rerouted Transcriptomic identified global gene expression changes. This study forms basis for further engineering strains achieve outstanding performance.

Язык: Английский

Процитировано

0