Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(45), С. 25330 - 25339
Опубликована: Окт. 23, 2024
It
is
of
particular
importance
to
develop
an
effective
method
that
possesses
several
merits
simultaneously
rapid,
ultrasensitive,
portable,
and
on-site
detection
potential
for
food
safety
detection.
Herein,
we
propose
a
clickase-mediated
immunoassay
based
on
nanopore
bionic
signal
labels
the
ricin.
The
introduction
Cu/Cys
clickase
effectively
addressed
inherent
limitations
natural
enzymes
colorimetric
output,
respectively.
Using
this
method,
can
be
easily
formed
through
DNA
Gram-positive
bacterial
cell
wall
terminal
peptide
fragments
(labeled
by
alkynyl
azide,
respectively)
vancomycin.
Translocation
D-P@vancomycin
generated
highly
specific
oscillation
current
traces.
This
showed
great
superior
analytical
performance
owing
combination
specificity
antibodies,
high
CuAAC
click
reaction
catalytic
efficiency
clickase,
ultrasensitivity
nanopore,
resolution
D-P@vancomycin.
Moreover,
practical
applicability
established
was
also
verified,
achieving
limit
(LOD)
down
200.9
ag/mL
with
wide
linear
relationship
under
optimized
conditions.
In
conclusion,
promising
Analytical Chemistry,
Год журнала:
2025,
Номер
97(2), С. 1357 - 1365
Опубликована: Янв. 7, 2025
The
development
of
a
sensory
signal
amplification
approach
is
very
crucial
for
rapid
and
precise
detection
aflatoxin
B1
(AFB1).
However,
such
approaches
remain
scarce
due
to
the
weak
interactions
between
AFB1
sensing
probes.
Herein,
first
example
dual-excitation
fluorescent
platform
antibody-free
reported,
which
assembled
by
an
ordered
π–π
stack
cationic
perylene
derivative
(PTHA)
tris(2,2′-bipyridine)ruthenium(II)
[Ru(bpy3)2+].
Taking
advantage
stepwise
assembly
multiple
binding
sites
nanoprobe,
its
ability
capturing
significantly
improved
driven
noncovalent
interactions.
Interestingly,
mode
with
superposition
self-calibration
activated
in
supramolecular
coassembly
process.
Under
excitation
365
nm
440
nm,
exhibits
specific
recognition
toward
limit
determined
be
0.12
ng
mL–1.
Notably,
demonstrates
exceptional
sensitivity
enhancements
106-fold,
revealing
that
self-calibrated
reference
improves
accuracy
analytical
method
significantly.
applications
our
not
only
crack
problem
via
strategy
but
also
provide
universal
sensitization
ultrasensitive
analysis
complex
environments.
Chemosensors,
Год журнала:
2025,
Номер
13(4), С. 141 - 141
Опубликована: Апрель 11, 2025
Aflatoxin
B1
(AFB1)
exhibits
high
toxicity
and
has
the
potential
to
induce
cancer,
deformities,
mutations.
It
is
therefore
highly
desirable
that
sensitive
straightforward
methods
for
detecting
AFB1
be
developed.
In
this
study,
due
specific
adsorption
capacity
of
aptamers,
we
applied
a
sensing
strategy
based
on
quantum
dots
(QDs)
carboxyl-functionalized
graphene
oxide
(FGO)
construct
simple
fluorescence
quenching
platform.
FGO
CdTe
QDs
modified
with
aptamers
cause
FRET
effect
produces
yellow-green
quenching.
When
present,
form
complexes
it
leave
platform,
resulting
in
recovery.
used
aptasensor
wide
detection
range
0.05
150
ng/mL
low
limit
(LOD)
8.2
pg/mL.
The
average
recoveries
peanut
pure
milk
samples
ranged
from
94.5%
107.0%.
also
exhibited
advantages
operation,
cost,
good
stability.
reported
here
can
thus
serve
as
candidate
rapid
AFB1.
