Immobilization of cells expressing d-allulose 3-epimerase and their application in d-allulose bioproduction with the assistance of boric acid

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106027 - 106027

Опубликована: Янв. 1, 2025

Язык: Английский

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Green biotechnological synthesis of rare sugars/alcohols: D-allulose, Allitol, D-tagatose, L-xylulose, l-ribose

Food Research International, Год журнала: 2025, Номер 206, С. 116058 - 116058

Опубликована: Фев. 25, 2025

Язык: Английский

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The use of isomerases and epimerases for the production of the functional sugars mannose, allulose and tagatose from Fructose

World Journal of Microbiology and Biotechnology, Год журнала: 2025, Номер 41(4)

Опубликована: Апрель 1, 2025

Язык: Английский

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Development and construction of a novel Bacillus subtilis autoinducible extracellular expression system based on a LuxI/R device

Microbial Cell Factories, Год журнала: 2025, Номер 24(1)

Опубликована: Апрель 19, 2025

Microbial chassis expression systems are valuable tools in biotechnology and synthetic biology, Bacillus subtilis is an important industrial microbial chassis. Quorum sensing (QS)-based dynamic regulation widely used to automatically activate gene response changes cell density. The main bottleneck currently limiting the use of exogenous QS B. for efficient autoinducible extracellular recombinant proteins their low level expression. A novel system based on LuxI/R-type (lux system) Vibrio fischeri was developed which lux enhanced by engineering module promoters. By promoter SPluxI core region (- 10 - 35 elements) critical (UP spacer elements), RPluxIR6 box copy number original LuxI/R device (S0-R0), high-expression Sc-R2 construct obtained. After shake flask 3-L fermenter fermentation, amylase activity obtained with 2.7- 3.1-fold greater, respectively, than that well-characterized Pveg. achieved 2.6-fold greater S0-R0 when either levansucrase or invertase as a reporter protein. Overall, this study showed good generalizability application potential industrial-scale fermentation. To our knowledge, first report sequence RPluxIR6. This further expands biology.

Язык: Английский

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Translation regulation in Bacillus subtilis and its applications in heterologous protein expression: A review

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 311, С. 143653 - 143653

Опубликована: Апрель 29, 2025

Язык: Английский

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Mining and identifying a D-mannose isomerase with high fructose isomerization activity and its expression in Bacillus subtilis for D-mannose production

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 311, С. 143724 - 143724

Опубликована: Май 1, 2025

Язык: Английский

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Establishment of the CRISPR-Cpf1 gene editing system in Bacillus licheniformis and multiplexed gene knockout

Synthetic and Systems Biotechnology, Год журнала: 2024, Номер 10(1), С. 39 - 48

Опубликована: Авг. 8, 2024

is a significant industrial microorganism. Traditional gene editing techniques relying on homologous recombination often exhibit low efficiency due to their reliance resistance genes. Additionally, the established CRISPR technology, utilizing Cas9 endonuclease, faces challenges in achieving simultaneous knockout of multiple To address this limitation, CRISPR-Cpf1 system has been developed, enabling multiplexed across various microorganisms. Key efficient capability rigorous screening highly effective expression elements achieve conditional protein Cpf1. In study, we employed mCherry as reporter and harnessed P

Язык: Английский

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Characterization of Runella zeae D-mannose 2-epimerase and its expression in Bacillus subtilis for D-mannose production from D-glucose

Enzyme and Microbial Technology, Год журнала: 2024, Номер 181, С. 110506 - 110506

Опубликована: Сен. 6, 2024

Язык: Английский

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Structure prediction of alcohol dehydrogenase from Gluconobacter frateurii and its application in efficient biotransformation of D-allulose from allitol

Food Bioscience, Год журнала: 2024, Номер unknown, С. 105626 - 105626

Опубликована: Дек. 1, 2024

Язык: Английский

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