Structure prediction of alcohol dehydrogenase from Gluconobacter frateurii and its application in efficient biotransformation of D-allulose from allitol DOI
Xin Wen, Huibin Lin,

Xi-xian Xu

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105626 - 105626

Published: Dec. 1, 2024

Language: Английский

Immobilization of cells expressing d-allulose 3-epimerase and their application in d-allulose bioproduction with the assistance of boric acid DOI
Xin Wen, Huibin Lin, Guangwen Liu

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106027 - 106027

Published: Jan. 1, 2025

Language: Английский

Citations

1
Green biotechnological synthesis of rare sugars/alcohols: D-allulose, Allitol, D-tagatose, L-xylulose, l-ribose DOI
Xin Wen,

Mesfin Angaw Tesfay,

Yuhang Ning

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 206, P. 116058 - 116058

Published: Feb. 25, 2025

Language: Английский

Citations

0
The use of isomerases and epimerases for the production of the functional sugars mannose, allulose and tagatose from Fructose DOI

Junya Yang,

Yingying Zhu, Wei Xu

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(4)

Published: April 1, 2025

Language: Английский

Citations

0
Development and construction of a novel Bacillus subtilis autoinducible extracellular expression system based on a LuxI/R device DOI Creative Commons

Bin Wang,

Keyi Wang, Xin Zhao

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: April 19, 2025

Microbial chassis expression systems are valuable tools in biotechnology and synthetic biology, Bacillus subtilis is an important industrial microbial chassis. Quorum sensing (QS)-based dynamic regulation widely used to automatically activate gene response changes cell density. The main bottleneck currently limiting the use of exogenous QS B. for efficient autoinducible extracellular recombinant proteins their low level expression. A novel system based on LuxI/R-type (lux system) Vibrio fischeri was developed which lux enhanced by engineering module promoters. By promoter SPluxI core region (- 10 - 35 elements) critical (UP spacer elements), RPluxIR6 box copy number original LuxI/R device (S0-R0), high-expression Sc-R2 construct obtained. After shake flask 3-L fermenter fermentation, amylase activity obtained with 2.7- 3.1-fold greater, respectively, than that well-characterized Pveg. achieved 2.6-fold greater S0-R0 when either levansucrase or invertase as a reporter protein. Overall, this study showed good generalizability application potential industrial-scale fermentation. To our knowledge, first report sequence RPluxIR6. This further expands biology.

Language: Английский

Citations

0
Mining and identifying a D-mannose isomerase with high fructose isomerization activity and its expression in Bacillus subtilis for D-mannose production DOI
Qiang Wei, Yi Lv, Mingqiang Chen

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143724 - 143724

Published: May 1, 2025

Language: Английский

Citations

0
Translation regulation in Bacillus subtilis and its applications in heterologous protein expression: A review DOI
Tian Gan,

Yidi Liu,

Ying Qiao

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143653 - 143653

Published: April 29, 2025

Language: Английский

Citations

0
Establishment of the CRISPR-Cpf1 gene editing system in Bacillus licheniformis and multiplexed gene knockout DOI Creative Commons

Suxin Liu,

Fengxu Xiao,

Youran Li

et al.

Synthetic and Systems Biotechnology, Journal Year: 2024, Volume and Issue: 10(1), P. 39 - 48

Published: Aug. 8, 2024

is a significant industrial microorganism. Traditional gene editing techniques relying on homologous recombination often exhibit low efficiency due to their reliance resistance genes. Additionally, the established CRISPR technology, utilizing Cas9 endonuclease, faces challenges in achieving simultaneous knockout of multiple To address this limitation, CRISPR-Cpf1 system has been developed, enabling multiplexed across various microorganisms. Key efficient capability rigorous screening highly effective expression elements achieve conditional protein Cpf1. In study, we employed mCherry as reporter and harnessed P

Language: Английский

Citations

2
Characterization of Runella zeae D-mannose 2-epimerase and its expression in Bacillus subtilis for D-mannose production from D-glucose DOI
Yuhan Wei, Wei Xu, Wenli Zhang

et al.

Enzyme and Microbial Technology, Journal Year: 2024, Volume and Issue: 181, P. 110506 - 110506

Published: Sept. 6, 2024

Language: Английский

Citations

1
Structure prediction of alcohol dehydrogenase from Gluconobacter frateurii and its application in efficient biotransformation of D-allulose from allitol DOI
Xin Wen, Huibin Lin,

Xi-xian Xu

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105626 - 105626

Published: Dec. 1, 2024

Language: Английский

Citations

0